Ask about this productRelated genes to: USP4 antibody
- Gene:
- USP4 NIH gene
- Name:
- ubiquitin specific peptidase 4
- Previous symbol:
- UNP
- Synonyms:
- Unph
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 1998-05-29
- Date modifiied:
- 2016-10-05
Related products to: USP4 antibody
Related articles to: USP4 antibody
- Renal interstitial fibrosis (RIF), driven by persistent TGF-β signaling, is a hallmark of progressive kidney disease. Because deubiquitinases (DUBs) like USP4 stabilize TGF-β type I receptor (TβRI) to prolong signaling, targeting USP4 represents a promising antifibrotic strategy. - Source: PubMed
Publication date: 2026/04/03
Fan JianhuiLiu YuqiZhan ShihongLing HuayuYan JiaminXie LeyiWang WeiguangXiao XiaoLi Ao - Colorectal cancer is a major cause of cancer-related mortality, and its clinical management is still limited by recurrence, metastasis, and therapy resistance. Although ferroptosis is increasingly recognized as a therapeutically relevant vulnerability in colorectal cancer, the upstream regulators that link malignant signaling to ferroptosis-related homeostasis remain poorly defined. TRIM21 is a multifunctional E3 ubiquitin ligase with emerging roles in cancer biology, but its involvement in USP4/TGF-β-associated regulation and ferroptosis-related phenotypes in colorectal cancer is not well understood. Public transcriptomic datasets were analyzed to evaluate the clinical significance of TRIM21 expression across different clinicopathological categories. Gain- and loss-of-function approaches were applied in HCT116 cells to evaluate proliferation, migration, and invasion using CCK-8, wound-healing, and Transwell assays. We examined the relationship between TRIM21 and USP4 by co-immunoprecipitation and a rescue design involving TRIM21 silencing with USP4 re-expression. Ferroptosis-associated markers (SLC7A11 and GPX4) were evaluated using immunoblotting, and ferroptosis-related biochemical indices (Fe, SOD activity, and MDA content) were quantified. Ferrostatin-1 was used to pharmacologically investigate ferroptosis under TRIM21/USP4 perturbations. TRIM21 was clinically associated with advanced clinicopathological features and was elevated in colorectal cancer cell models. Functional studies revealed that TRIM21 promotes proliferative and invasive/migratory phenotypes, accompanied by coordinated changes in USP4 and TGFB1 transcript levels. USP4 re-expression in TRIM21-silenced cells partially restored malignant traits and reshaped ferroptosis-associated molecular and biochemical readouts. Pharmacological inhibition of ferroptosis modulated TRIM21/USP4-linked phenotypes and corresponding ferroptosis-related indices. These findings identify TRIM21 as a clinically relevant regulator that links USP4/TGF-β-associated signaling to ferroptosis-related homeostasis, thereby promoting malignant behaviors in colorectal cancer and providing a targetable vulnerability for therapeutic development. - Source: PubMed
Publication date: 2026/03/19
Wang QianYao LinWang BiboLiu XiufengChu Xiaoyuan - Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality. This study examines whether the deubiquitinase USP4 promotes NSCLC progression by stabilizing PAF1 and opposing the E3 ligase CNOT4. - Source: PubMed
Publication date: 2026/03/19
Chen ShaomuWang YuxuanHan SongZhang Biao - Local progression and metastasis remain the foremost impediments to long‑term survival among patients with colorectal cancer (CRC). long non‑coding RNAs (lncRNAs) have a pivotal role in the advancement of colorectal malignancies. The aim of the present study was to elucidate the functional role and underlying molecular mechanisms of the lncRNA AL445238.2 in CRC progression. In the present study, overexpression/knockdown lentiviral vectors, protein half‑life assays and co‑immunoprecipitation assays were used to explore the regulatory relationship among AL445238.2, ubiquitin‑specific protease 4 (USP4) and BCL2, combined with Transwell assays, sphere formation assays and subcutaneous xenograft models to demonstrate their effects on colon cancer proliferation and stemness both and . The experimental findings revealed that AL445238.2 was highly expressed in CRC cells. AL445238 overexpression significantly enhanced the proliferation of DLD1 and SW480 cells, reduced extracellular lactate dehydrogenase release, diminished apoptotic activity and increased cellular stemness, as evidenced by increased CD133 expression and augmented tumor sphere formation, together with enhanced mitochondrial activity. RNA pulldown and immunofluorescence assays further demonstrated a direct interaction between AL445238.2 and USP4, with the two synergistically modulating the expression of the anti‑apoptotic protein Bcl2 and the pro‑apoptotic protein BAX to suppress apoptosis. Moreover, in assays, USP4 independently promoted cell proliferation, sustained stemness and enhanced mitochondrial function, thereby increasing tumor growth. Collectively, the findings of the present study revealed that AL445238.2, through its interaction with USP4, orchestrated the regulation of cell proliferation, apoptosis, stemness maintenance and migration in CRC cells, offering novel insights into the role of lncRNAs in cancer progression and highlighting potential therapeutic targets. - Source: PubMed
Publication date: 2026/03/06
Lin HualongFeng JieniChen PeiruiHu JialinZhu LinjiaYuan Shaofei - In this research, we extended our examination of the US pharmacopeia apparatus 4 (USP-4) application for the development of an in vitro acellular dissolution protocol for man-made vitreous fibres (MMVF). The aim of this study was to achieve robust differentiation in solubility of glass and stone wool fibres using a USP-4 closed-loop configuration. For this, synthetic lung fluids representing extra-cellular conditions of the lungs (pH 7.4) and the environment in the phagolysosome (pH 4.5) were evaluated and tested in closed-loop dissolution experiments with multiple fibres across several labs. Our results showed that it was possible to differentiate between high- and low-solubility fibres using all candidate fluids. However, we found that it was crucial to limit the pH variability and to adapt the levels of organics in the fluid, as they impacted the dissolution of the fibres. Our findings indicated good inter-lab variability of an in vitro acellular biosolubility testing with the USP-4. For further development of the USP-4 closed loop configuration protocol, we propose focusing on phosphate-based buffers for pH 7.4 and acetate buffers or PSF + 10 mg/L citrate for pH 4.5. - Source: PubMed
Publication date: 2026/02/19
Okhrimenko Denis VHiéronimus LéaHoffman John WKessler HeatherDan Edson Jacquemin MaximeHérault QuentinDrnovšek NatašaKovačič UrškaAixart JordiMascaraque NereaRickabaugh KeithWiggins BrandonMadl Amy KSolvang Mette