Ask about this productRelated genes to: BRS3 antibody
- Gene:
- BRS3 NIH gene
- Name:
- bombesin receptor subtype 3
- Previous symbol:
- -
- Synonyms:
- BB3, BB3R
- Chromosome:
- Xq26.3
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-08
- Date modifiied:
- 2016-06-07
Related products to: BRS3 antibody
Related articles to: BRS3 antibody
- : High recurrence rates and intensive lifelong surveillance make bladder cancer among the costliest malignancies to treat. Although Bacillus Calmette-Guérin (BCG) immunotherapy is the standard treatment for high-risk non-muscle-invasive bladder cancer (NMIBC), up to 50% of patients fail to respond, and predictive biomarkers are lacking. Molecular profiling has established three BCG response subtypes (BRS1-3), with BRS3 characterized by an immunosuppressive, BCG-resistant phenotype; however, these features have not been validated at single-cell spatial resolution. : We applied imaging mass cytometry (IMC) to 82 BCG-treated high-risk NMIBC samples and performed (i) single-cell IMC with unsupervised clustering to identify phenotypic cell clusters and quantify cluster abundances and (ii) a convolutional neural network-based gated attention multiple instance learning model trained on IMC images (IMC-GA-MIL) to predict BCG response. Cluster abundances were summarized using II (immune composition within the immune compartment), TT (tumor phenotypic composition), and IT (immune/stromal abundance relative to tumor cells) indices. : Single-cell IMC identified 18 distinct phenotypic cell clusters. In BCG responders, immune cells localized within the tumor compartment were enriched and independently protective (HR 0.67, 95% CI 0.49-0.92). BCG nonresponse was associated with a higher abundance of fibroblast-dominant clusters relative to tumor cells (IT index). Plasma cell-dominant clusters were the strongest predictors of progression (II index HR 2.28, 95% CI 1.37-3.79; IT index HR 1.25, 95% CI 1.06-1.48). The IMC-GA-MIL model predicted BCG response with 90% accuracy (9/10) and identified myeloid- and T-cell-associated marker patterns involving CD14, CD11b, CD68, CD8, and FOXP3 as the most informative contributors. : Spatial single-cell profiling and IMC-GA-MIL identify spatial immune and stromal features associated with BCG failure. However, findings from both analyses should be considered exploratory and will require validation in larger, independent cohorts. - Source: PubMed
Publication date: 2026/03/13
Lillesand MelindaAustdal MarieMroz JakubSkaland IvarGudlaugsson EinarJong Florus C deZuiverloon Tahlita C MEngan KjerstiJanssen Emiel A M - The paraventricular hypothalamus (PVH) controls behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for centrally projecting PVH neuron populations remain largely undefined, and a complete census of PVH cell types has not been established. Therefore, we performed extensive single-cell/nucleus RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic atlas resolves 26 Sim1 and 29 GABAergic neuron populations from the PVH and surrounding areas. Additionally, projection-based profiling identified neurons that project to the parabrachial region (PB) and spinal cord, helping to determine PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and silencing them causes obesity. Together, this atlas contributes high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis. - Source: PubMed
Publication date: 2026/01/23
Li YuxiButler Trevor CNardone StefanoJacobs Christopher LDouglass Amelia MMadara Joseph CMcDonough Miriam CTao JenkangLowenstein Elijah DWang LuhongPant DeeptiWalker Samuel JWang AnnetteSrinivasan HariniYang ZongfangCampbell John NTsai Linus TLowell Bradford BResch Jon M - Fever or inflammation state may enhance the Brugada syndrome (BrS) phenotype in some but not all patients. However, the underlying mechanism in human cardiomyocytes has not yet been clarified. - Source: PubMed
Publication date: 2025/12/01
Li YingruiRose LenaPrädel TimoKleinsorge MandyFan XuehuiMeng ZenghuiYan ChenLiu RuiLei XinhaoZhao BinyiYang GuoqiangLiao ZhenxingDinkel HendrikBusley Alexandra ViktoriaZhong RujiaZhang FengXu QiangMaywald LasseAweimer AssemHuang MengyingMoscu-Gregor AlexanderHamdani NazhaSchneider LucaZemedi YeweynwuhaZhazykbayeva SaltanatHohn AlyssaYang ZhenQiao LinMügge AndreasCyganek LukasZhou XiaoboAkin IbrahimEl-Battrawy Ibrahim - Bombesin receptor subtype 3 (BRS3) has long been an enigmatic orphan G protein-coupled receptor, but has now emerged as a key orchestrator of energy homeostasis, primarily through its actions within the hypothalamus. This review charts the journey of its characterization, synthesizing multiscale evidence from anatomical mapping to cutting-edge spatial transcriptomics to reveal a network of functionally specialized neural circuits. The discussion highlights how distinct BRS3-expressing neuronal populations within the hypothalamus and related preoptic areas govern discrete metabolic functions: those in the preoptic area drive potent thermogenic and cardiovascular responses, while others in the paraventricular hypothalamus specifically regulate food intake. This functional segregation, however, creates a critical translational dilemma. The therapeutic potential of central BRS3 modulation is tempered by significant on-target cardiovascular risks, directly linked to these circuits. In contrast, peripheral targeting offers a promising, albeit more modest, avenue with a more favorable safety profile. To resolve this, this review proposes a circuit-based rationale for a stratified therapeutic strategy: prioritizing peripherally-restricted agonists for broad metabolic disorders and reserving central interventions for highly specific indications where the benefits demonstrably outweigh the risks. Ultimately, this review argues that a precise understanding of BRS3's circuit-specific neurochemistry is paramount not only to unlock its full therapeutic potential but also to proactively navigate its inherent risks, thereby guiding the development of safer, more effective treatments for obesity and diabetes. - Source: PubMed
Publication date: 2025/11/25
Zhang ZixuHu ShengruGeng ShuyanXu TianxiangSun ChuanyaoWei WenqiShao WangqiDuan YikeXie WeiMu Mingdao - The paraventricular hypothalamus (PVH) controls many behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for centrally-projecting PVH neuron populations remain largely undefined, and a complete census of PVH cell types has not been established. Therefore, we performed extensive single-cell/nucleus RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding areas, revealing multiple subtypes not described previously and distinct transcriptional programs between neuroendocrine and centrally-projecting neurons. Additionally, projection-based profiling determined neuronal subtypes that project to the parabrachial region (PB) and spinal cord, helping to identify PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, activation of PB-projecting PVH neurons expressing bombesin-like receptor 3 (Brs3) reduces food intake and silencing them causes obesity. Together, this atlas contributes high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis. - Source: PubMed
Publication date: 2025/10/21
Resch JonLi YuxiButler TrevorNardone StefanoJacobs ChristopherDouglass AmeliaMadara JosephMcDonough MiriamTao JenkangLowenstein ElijahWang LuhongPant DeeptiWalker SamuelWang AnnetteSrinivasan HariniYang ZongfangCampbell JohnTsai Linus TLowell Bradford