Ask about this productRelated genes to: NPFF1 antibody
- Gene:
- NPFFR1 NIH gene
- Name:
- neuropeptide FF receptor 1
- Previous symbol:
- GPR147
- Synonyms:
- OT7T022, NPFF1R1
- Chromosome:
- 10q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-05-27
- Date modifiied:
- 2016-10-05
Related products to: NPFF1 antibody
Related articles to: NPFF1 antibody
- Dabry's sturgeon (Acipenser dabryanus) is one of the precious large fish species unique to the upper reaches of the Yangtze River in China. Investigating the molecular mechanisms that regulate the hypothalamic-pituitary-gonadal axis is crucial for the conservation of Dabry's sturgeon. In the present study, transcriptome sequencing and analysis were conducted on hypothalamus, pituitary, and gonad tissues of male and female Dabry's sturgeon. Raw sequence data were compiled and duplicate entries were eliminated, yielding 603,597 unigenes with an average length of 843 base pairs and an N50 value of 1056 bp. In the hypothalamus, the representative up-regulated pathways and genes include fatty acid biosynthesis (fasn, acsl) and fatty acid metabolism (acd), while down-regulated pathways and genes include neuroactive ligand-receptor interactions (lhcgr, npffr1, gabrd). In the pituitary, the representative up-regulated pathways and genes include neuroactive ligand-receptor interactions (drd2, trhr), while down-regulated pathways and genes are unsaturated fatty acid biosynthesis (elovl6). In the gonad, the representative up-regulated pathways and genes include unsaturated fatty acid biosynthesis (fads2), tryptophan metabolism (cyp1a1 and cyp1b1), while down-regulated pathways and genes are ovarian steroid production (fshr, igf1, and cyp11a). These representative pathways and genes play an important role in the gonadal development of Dabry's sturgeon. These potential determinants provide a glimpse into of the molecular mechanisms of hypothalamic-pituitary-gonadal axis regulation in Dabry's sturgeon. CLINICAL TRIAL NUMBER: All fish handling and experimental procedures in this study have been approved by the Chinese Sturgeon Research Institute of China Three Gorges Corporation, and the Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes (Chinese Sturgeon Research Institute of China Three Gorges Corporation). - Source: PubMed
Publication date: 2025/11/08
Shi XuetaoLi YangHuang HongtaoXiao KanYang Jing - Neuropeptide FF receptors 1 and 2 (NPFFR1 and NPFFR2) are RF-amide peptide receptors that couple to G proteins and regulate pain, opioid tolerance, and metabolism. Despite their physiological significance, their ligand selectivity and activation mechanisms remain unclear. Using cryoelectron microscopy, we resolved four NPFFR1 and NPFFR2 structures bound to NPFF or NPVF, revealing conserved C-terminal RF-amide interactions within the orthosteric pocket and N-terminal variations driving subtype specificity. Structural and mutagenesis analyses identified ECL2 and the receptor N terminus as key determinants of NPVF-NPFFR1 and NPFF-NPFFR2 selectivity. Additionally, the structures elucidate the activation mechanism and uncover distinct G-coupling features between NPFFR subtypes. These findings provide molecular insights into peptide recognition and receptor activation within the RF-amide family, offering a structural framework for designing selective NPFFR modulators to treat pain, addiction, and metabolic disorders with enhanced specificity and reduced off-target effects. - Source: PubMed
Publication date: 2025/08/19
Li XinzhuZhang HengHu WenWu KaiLi ShuaiJin SanshanYin YulingYuan QingningXu H EricPan BenxunJiang Yi - Type 2 diabetes (T2D) represents a complex and multifactorial disorder, and efforts to discover its treatment are necessary. Browning of white adipose tissue (WAT) as a therapeutic target for diabetes seems to be induced by exercise through neuropeptide FF (NPFF) signaling in the hypothalamus and adipose tissue. This study aimed to explore the role of endurance training on the browning of WAT by assessing the expression of the gene and protein of NPFF and its receptors in the hypothalamus and adipose tissue. - Source: PubMed
Mousavi Syiedeh MaryamGorji Fatemeh ZahraFallahmohammadi ZiyaNasiri KhadijehAkbari Abolfazl - Neuropeptide FF Receptor 2 (NPFFR2), a G-protein-coupled receptor, plays a role in pain modulation and diet-induced thermogenesis. While NPFFR2 is strongly activated by neuropeptides FF (NPFFs), it shows low activity in response to RF-amide-related peptides (RFRPs), despite the peptides belonging to a shared family. In contrast, NPFFR1, which shares high sequence similarity with NPFFR2, is activated by RFRPs and regulates reproductive hormone balance. The molecular basis for these receptor-specific interactions with their RF-amide peptides remains unclear. Here, we present cryo-electron microscopy structures of NPFFR2 in its active state bound to the agonist RF-amide peptide hNPSF, and in its ligand-free state. Structural analysis reveals that the C-terminal RF-amide moiety engages conserved residues in the transmembrane domain, while the N-terminal segment interacts in a receptor subtype-specific manner. Key selectivity-determining residues in NPFFR2 are also identified. A homology model of NPFFR1 bound to RFRP, supported by mutagenesis studies, further validates this selectivity mechanism. Additionally, structural comparison between the inactive and active states of NPFFR2 suggests a TM3-mediated activation mechanism. These findings provide insights into RF-amide peptide recognition by NPFF receptors. - Source: PubMed
Publication date: 2025/03/24
Kim JeesooHong SooyoungLee HajinLee Hyun SikPark ChaeheeKim JinukIm WonpilChoi Hee-Jung - Neuropeptide FF (NPFF) is an endogenous octapeptide that was originally isolated from the bovine brain. It belongs to the RFamide family of peptides that has a wide range of physiological functions and pathophysiological effects. NPFF and its receptors, NPFFR1 and NPFFR2, abundantly expressed in rodent and human brains, participate in cardiovascular regulation. However, the expressions of NPFF and its receptors are not restricted within the central nervous system but are also found in peripheral organs, including the kidneys. Both NPFFR1 and NPFFR2 mainly couple to Gαi/o, which inhibits cyclic adenosine monophosphate (cAMP) production. NPFF also weakly binds to other RFamide receptors and the Mas receptor. Relevant published articles were searched in PubMed, Google Scholar, Web of Science, and Scopus. Herein, we review evidence for the role of NPFF in the regulation of blood pressure, in the central nervous system, particularly within the hypothalamic paraventricular nucleus and the brainstem, and the kidneys. NPFF is a potential target in the treatment of hypertension. - Source: PubMed
Publication date: 2024/12/11
Lee HewangFeranil Jun BJose Pedro A