human frizzled homolog 8,FZD8 ELISA Kit
- Known as:
- H. sapiens frizzled homolog 8,FZD8 Enzyme-linked immunosorbent assay test Kit
- Catalog number:
- 201-12-0643
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sunredbio SunBT Sun red bio
- Gene target:
- human frizzled homolog 8 FZD8 ELISA Kit
Related genes to: human frizzled homolog 8,FZD8 ELISA Kit
- Gene:
- FZD8 NIH gene
- Name:
- frizzled class receptor 8
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 10p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 1998-09-17
- Date modifiied:
- 2016-10-05
Related products to: human frizzled homolog 8,FZD8 ELISA Kit
Related articles to: human frizzled homolog 8,FZD8 ELISA Kit
- Two hundred Holstein dairy cows (milk yield of 34.5 ± 0.6 kg/d, 3.1 ± 1.2 lactations) were enrolled to investigate the role of WNTs signaling network in early pregnancy loss. The ISG15 mRNA abundance of uterine luminal cells obtained via brushing on day 16 after service was used to determine initial pregnancy status, with confirmation via ultrasound on days 32 and 60. The mRNA abundance of cells obtained from uterine brushing for WNT2, WNT5A, WNT7A, WNT11, frizzled receptors (FZD), dickkopf proteins (DKK1), Kremen and LDL receptor related protein (LRP 5/6), lymphoid enhancer-binding factor 1 (LEF1) and transcription factor 7 (TCF) was determined at day16 after insemination. Relative mRNA abundance of DKK1 (1.98-fold), WNT5A (2.67-fold), WNT7A (1.83-fold) and WNT11 (2.16-fold) genes in pregnant cows were significantly greater than in cows with early embryonic mortality (P < 0.05). In contrast, relative mRNA abundance of WNT2 (3.35-fold) and FZD6 (5.50-fold) genes were significantly greater in pregnant-embryo loss cows compared to pregnant cows on day 16 (P < 0.05). The relative mRNA abundance of LRP5, LRP6, LEF1, FZD3, FZD5, FZD8 and TCF genes were not affected by pregnancy status although their variances were lower; however, there was a negative correlation among ISG15 and these genes. In conclusion, differences in mRNA abundance of WNT signaling genes in uterine brushing samples are associated with pregnancy status in lactating dairy cows and may contribute to minimizing early bovine pregnancy losses. - Source: PubMed
Publication date: 2026/06/04
Dirandeh EAnsari-Pirsaraei ZThatcher W W - Recognition of Wnt proteins by Frizzled (Fzd) receptors and the low-density lipoprotein receptor-related protein 5/6 (LRP5/6) co-receptor is essential for canonical Wnt signaling. It remains enigmatic how Wnt simultaneously interacts with Fzd and LRP5/6 and activates intracellular Wnt/β-catenin signaling. Here, we report cryo-electron microscopy (cryo-EM) structures of Wnt3a/Fzd8/LRP6 extracellular complexes captured in a 2:4:2 stoichiometry, consisting of a Wnt3a-Wnt3a homodimer, whereby each Wnt3a monomer binds to two Fzd8 receptors and one LRP6 co-receptor. This implies that Wnt3a induces Fzd cystine-rich domain (Fzd-CRD) tetramerization, which in turn could promote recruitment of oligomeric Disheveled (Dvl) to Fzd on the cytoplasmic side. Indeed, mutations of key Wnt3a-Wnt3a interface residues abolish Fzd-LRP clustering and downstream signaling, supporting a critical role of Wnt3a-Wnt3a dimerization in Wnt signalosome assembly and signaling. Our structures also show how the Wnt3a N-helical domain recognizes the LRP6 extracellular domain (LRP6-ECD) E3 β-propeller, while the Wnt3a N-C hairpin interacts with the valley between LRP6-E3 and -E4 propellers, underpinning the development of targeted Wnt therapeutics. - Source: PubMed
Publication date: 2026/05/27
Yue DanSun GangyuCao YunlongLi HongyueYang YufengPan ZirunXue LuluZhang LuWang ZhizhiXu Wenqing - Hsa-miR-99a has been linked to the advancement of several malignancies, including colorectal cancer (CRC). This investigation seeks to elucidate its function and regulatory network in CRC. - Source: PubMed
Publication date: 2026/03/17
Yang XuejingZhang TingtingSun HuFeng HuijingSong Dong - Wnt ligands stimulate β-catenin-dependent (canonical) or β-catenin-independent (noncanonical) signaling, depending on which co-receptors are recruited to the Wnt receptor FZD. Both pathways are initiated by receptor oligomerization into signalosomes and involve a largely overlapping set of downstream effectors. To resolve the assembly of Wnt signalosomes with high spatiotemporal resolution for extended times, we developed single-molecule tracking and localization microscopy based on labeling with reversibly binding nanobodies (rbTALM). We engineered nanobody-tag pairs with finely tuned binding affinities to ensure single-molecule tracking with high fidelity while also permitting continuous exchange of photobleached labels. Multicolor rbTALM imaging enabled simultaneous tracking and super-resolution imaging of three different tagged Wnt co-receptors in the same cell for more than 1 hour at video rate. Time-lapse correlation analyses uncovered cooperative association of canonical (LRP6) and noncanonical (ROR2) Wnt co-receptors with FZD8 into a common, hybrid Wnt signalosome. These findings demonstrate the potential for rbTALM imaging for exploring nanoscale dynamics across millisecond to hour timescales and for deciphering the molecular dynamics that underlie signaling complex formation. - Source: PubMed
Publication date: 2026/03/03
Philippi MichaelDohle JuliaWatrinet IsabelleHoltmannspötter MichaelMiao YiLi JinyeBirkholz OliverRothbauer UlrichGarcia K ChristopherKurre RainerPiehler JacobYou Changjiang - FZD8, a GPCR, is involved in various physiological processes, such as cell differentiation, bone growth and stem cell regulation, by binding to various Wnt ligands. Though FZD receptors have low mutation rates in cancer, aberrant Wnt or FZD expression leads to deregulated Wnt/FZD signaling pathways that may result in tumorigenesis. The structural and functional effects of most of the coding and non-coding SNPs of human FZD8 gene are yet to explore. In the present study, we analyzed 450, 109, and 390 SNPs in the CDS, 3'UTR, and 5'UTR of the FZD8 gene, respectively, using advanced state-of-the-art bioinformatics tools to explore their structural and functional consequences in tumorigenesis. We identified 10 highly deleterious nsSNPs among which 6 nsSNPs were located within the Wnt1 binding CRD region of FZD8. Additionally, these nsSNPs were also predicted to affect post-translational modifications in FZD8. The highly deleterious variant P120Q, causes significant structural change in secondary structure of FZD8 mRNA. Structure based stability prediction revealed 4 destabilizing variants among 6 highly deleterious variants. Wnt1 binds most strongly with FZD8-CRD among other FZD-CRDs. MD simulation analyses revealed that the binding energies of the mutated complexes were less stabilizing compared to the wild-type Wnt1-FZD8-CRD complex, with the P74L and A119E complexes predicted to be the most destabilizing. SNPs, rs1408188233 (34.T > C) in 3'UTR and rs1588706985 in 5'UTR may lead to AGO2 mediated and E2F6 mediated silencing of the FZD8 gene, respectively, whereas nsSNPs-rs1322411573 and rs1410965895 in the CDS may abolish miRNA-mediated gene silencing. Differential expression of FZD8 was observed across various normal, tumor, and metastatic tissues, and its deregulation was associated with reduced survival outcomes in patients with different types of cancer. Additionally, several biomarkers involving FZD8 mutations have been identified in patients with gastric cancer, multiple myeloma, and uterine cancer. Furthermore, these computationally prioritized high risk SNPs of FZD8 can be investigated in population based genetic studies and may serve as potential targets for future drug development against FZD8-associated diseases. - Source: PubMed
Publication date: 2026/02/24
Mondal AmaleshPaul DebaratiMondal TithiGoswami Achintya Mohan