Gab1 (Phospho_Tyr659) Antibody
- Known as:
- Gab1 (Phospho_Tyr659) Antibody
- Catalog number:
- E012082-2
- Product Quantity:
- 100ug
- Category:
- Antibodies
- Supplier:
- EnoGene
- Gene target:
- Gab1 (Phospho_Tyr659) Antibody
Related genes to: Gab1 (Phospho_Tyr659) Antibody
- Gene:
- GAB1 NIH gene
- Name:
- GRB2 associated binding protein 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 4q31.21
- Locus Type:
- gene with protein product
- Date approved:
- 1996-12-18
- Date modifiied:
- 2016-10-05
Related products to: Gab1 (Phospho_Tyr659) Antibody
Related articles to: Gab1 (Phospho_Tyr659) Antibody
- Obesity is a major risk factor for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and its progressive form, Metabolic Dysfunction-Associated Steatohepatitis (MASH). Bacterial cyclodipeptides (CDPs) have previously been shown to modulate PI3K/Akt/mTOR signaling and transcriptional programs involved in lipid and cholesterol metabolism, highlighting their potential to regulate metabolic homeostasis. Given the central role of the PI3K/Akt/FoxO1 pathway in regulating aquaporins, inflammatory cytokines, and metabolic homeostasis during obesity and MASLD, the therapeutic potential of CDPs was evaluated in a rat model of MASLD. Treatment of obese animals with CDPs decreased body weight and abdominal and thoracic circumferences. The obesogenic diet induced liver damage after the 34th week, which was alleviated by ten weeks of CDP treatment administered three times a week. This reduced the number and size of lipid droplets and inflammatory infiltrates. Additionally, CDPs improved parameters associated with atherogenic dyslipidemia and metabolic dysfunction, including triglycerides and cholesterol, as well as hepatic injury markers. These changes were accompanied by restoration of mitochondrial membrane potential and a significant reduction in mitochondrial ROS generation. CDP treatment in obese animals restored protein expression levels of aquaporins AQP1, AQP5, AQP8, and AQP9 and downregulated the expression of the inflammatory markers NF-κB and IL-1β. Interestingly, the downstream cellular mediator GAB1 from the PI3K/Akt/FoxO1 pathway was also downregulated. These findings demonstrate that CDP treatment restored the expression of glycerol/water transporting aquaporins altered during obesity and MASLD. Overall, CDPs ameliorated MASLD in obese animals through coordinated modulation of lipid metabolism, mitochondrial function, aquaporin expression, and PI3K/Akt/FoxO1 signaling associated with inflammation, supporting their potential as therapeutic candidates for metabolic liver disease. - Source: PubMed
Publication date: 2026/08/12
Figueroa-Guzmán CitlaliGaona-García Roxana YCampos-Morales Marlene EMartínez-Alcantar LorenaCampos-García Jesús - BMP2 serves as a pivotal regulator of skeletal development, homeostasis, and repair. Previously, we demonstrated that Gαi1/3 (G protein subunit alpha i1/3) is not only involved in G protein-coupled receptor (GPCR) signal transduction but also acts as a key mediator of tyrosine kinase receptor signaling. However, its role in bone formation and BMP2 signaling remains elusive. In this study, we report that the expression of Gαi1/3 is significantly downregulated in the bone tissue of patients with senile osteoporosis. Gαi1/3 double knockout mice exhibited delayed skeletal development and reduced bone mass. Mechanistically, Gαi1/3 is required for BMP2 signal transduction, as its deletion impaired BMP2 downstream signaling. In BMSCs (bone marrow-derived mesenchymal stem cells), Gαi1/3 knockout suppressed BMP2-induced osteogenesis, while overexpression enhanced it. In the femoral fracture model, knockdown of Gαi1/3 in BMSCs reduced the ability of BMP2 to promote fracture healing. Furthermore, in neonatal mice with conditional knockout of Gαi1/3 in BMSCs, skeletal development was delayed. Gab1 is indispensable for Gαi1/3-mediated BMP2 signaling, as Gαi1/3 binds to both the BMP2 receptor and Gab1 to mediate BMP2 biological function. Collectively, our findings reveal the critical role of the Gαi1/3-Gab1 axis in BMP2 signaling and osteogenesis, providing potential therapeutic targets for osteoporosis and bone repair. - Source: PubMed
Publication date: 2026/08/10
Shan HuajianBai ChaowenZhu YifeiXu ChenyangLi SonngtaoSun QizeShe ChangBai JinyuCao CongYang YitianZhou Xiaozhong - The biological function of intrinsically disordered proteins is frequently coupled to short linear motifs, which serve as protein binding sites. This goes often hand in hand with local transient structural element formation. The intrinsically disordered C-terminal region of the multi-site docking protein Grb2-associated binder 1 (Gab1) contains several well-characterized phosphotyrosine pairs, whereas the Tyr162 and Tyr183 epitopes have remained practically unstudied. Here, we combine computational prediction, circular dichroism and high-resolution NMR spectroscopy (chemical shift and relaxation analyses) to structurally characterize a Gab1 fragment containing residues 142-203. The here determined NMR structure shows an α-helix for residues Pro180 to Ile187, with Tyr183 positioned centrally, while all other parts of the fragment, including the region around Tyr162, are disordered. This helical conformation is maintained upon changes in pH, variation of peptide lengths and phosphorylation status. The inherent helix around Tyr183 distinguishes the local structural environment of Tyr162 and Tyr183 within the intrinsically disordered Gab1 tail and provides a structural framework for future studies on interaction partners and potential functional roles of this structural motif. - Source: PubMed
Dietrich AnneLewitzky MarcGruber TobiasBalbach JochenFeller Stephan MWeininger Ulrich - In non-small cell lung cancer (NSCLC) with anaplastic lymphoma kinase (ALK) rearrangement, bypass signaling activation commonly leads to resistance against alectinib. Identifying key molecular targets that integrate signals from resistance-driving kinases is crucial for overcoming this resistance. However, these targets have not yet been identified. - Source: PubMed
Publication date: 2026/07/23
Chen HengyiLin CaiyuHu ChenLi WenHe Yong - Medulloblastoma is a heterogeneous solid tumor, and its molecular characteristics are the most important prognostic factors for this neoplasm. Unfortunately, the molecular classification of MB-G3 and MB-G-4 medulloblastoma is very complex because of molecular similarity. Therefore, in this work, through unsupervised machine learning-based gene expression profiling, we identified a low molecular profile associated with four molecular groups of medulloblastoma. We performed medulloblastoma expression microarray data mining via the Partek Genomics Suite and Transcriptome Analysis Console (TAC), and we included a total of 25 fresh medulloblastoma tumors that were obtained and hybridized into HG U133 Plus 2.0 Array microarrays. To identify the molecular groups of the 25 patients, we compared them against classified patients, which were obtained from free repositories, and through data mining based on gene expression, compared the expression profiles of our patients. To do so, we performed an analysis via the least squares method via PCA. The molecular groups MB-WNT and MB-SHH were confirmed via immunohistochemistry via β-catenin, YAP1 and GAB1 antibodies in tissue fixed in formalin and embedded in paraffin, and another tissue section was placed on a Visium Spatial slide to perform spatial RNA-seq via Illumina NextSeq 2000 platform sequencers. The data obtained were analyzed with R. We identified the expression profiles associated with the four molecular groups and formed a reference set. Through unsupervised analysis via the least squares method, we assigned the molecular profiles of 25 patients with medulloblastoma, via the integration of bulk and spatial tumor molecular gene expression profiling analysis and with immunohistochemical findings, this strategy was fast and accurate. We observed correlations in three of the trials carried out and, in part, in one study, a patient who presented two tumor strains and two molecular signatures (SHH and G4), which led us to believe that this patient presented mixed phenotypic characteristics. Multigene expression profile analysis of medulloblastoma represents a significant advance in precision medicine; integrating different layers of transcriptomic information allows us to demonstrate underlying molecular changes in the four molecular groups that are essential for personalized therapy. - Source: PubMed
Publication date: 2026/06/24
Juárez-Méndez SergioVázquez-Jiménez AarónRamírez-Chiquito Josselen CarinaVillegas-Ruíz VanessaNiembro-Zuñiga Ana MariaFarfán-Morales José EduardoMarhx-Bracho AlfonsoKrötzsch EdgarRodríguez-Morales MiguelSegura-Solís EmmaPerezpeña-Diazconti MarioRidaura-Sanz CeciliaRivera-Luna RobertoEguía-Aguilar PilarResendis-Antonio OsbaldoMelendez-Zajgla Jorge