AKT1 (phospho_Thr450) Antibody
- Known as:
- AKT1 (phospho_Thr450) Antibody
- Catalog number:
- E011502-2
- Product Quantity:
- 100ug
- Category:
- Antibodies
- Supplier:
- EnoGene
- Gene target:
- AKT1 (phospho_Thr450) Antibody
Related genes to: AKT1 (phospho_Thr450) Antibody
- Gene:
- AKT1 NIH gene
- Name:
- AKT serine/threonine kinase 1
- Previous symbol:
- -
- Synonyms:
- RAC, PKB, PRKBA, AKT
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: AKT1 (phospho_Thr450) Antibody
Related articles to: AKT1 (phospho_Thr450) Antibody
- Persistent macrophage-mediated inflammation under hyperglycemic conditions is an important feature of the inflammatory microenvironment associated with diabetic wounds. α-Mangostin (α-MG), a natural xanthone derived from Garcinia mangostana, has been reported to exert anti-inflammatory activity in various experimental models. However, its effects on high-glucose-induced macrophage inflammation and oxidative stress remain insufficiently characterized. - Source: PubMed
Publication date: 2026/07/20
Cao PengWang YunweiXi MingfanLiu KaituoHui ShuoyiZhao PengLyu Guozhong - The fruits of Amomum maximum Roxb. (Zingiberaceae), traditionally used for gastrointestinal disorders, have been less phytochemically investigated compared to its rhizomes. In this study, four undescribed compounds (1-3,12) together with 15 known compounds were isolated from a 75% ethanol extract of A. maximum fruits, including eleven diarylheptanoids and eight labdane diterpenes. Their structures were unequivocally elucidated by spectroscopic methods, including NMR spectroscopy, computational NMR methods, and ECD. Compounds 1, 3-6, 10, and 11 significantly inhibited the release of NO, IL-6, and TNF-α in LPS/IFN-γ-stimulated RAW 264.7 macrophages. Furthermore, the integration of network pharmacology, molecular docking, and molecular dynamics simulations identified compounds 1, 3-6, 10, and 11 as promising candidates, demonstrating favorable binding affinity and stability with the active target AKT1. This study demonstrates that diarylheptanoids are the major anti-inflammatory components in A. maximum fruits, which not only validates the ethnopharmacological use of this herb, but also provides new anti-inflammatory agents derived from natural products. - Source: PubMed
Publication date: 2026/07/20
Zhao Jin-NaYu Shu-FeiDing Yan-JunLi BoChen LuWu Zhi-HangWang Jin-HuiWang Li-Bo - Acute lung injury (ALI) is a life‑threatening respiratory disease characterized by excessive inflammation, oxidative stress, and autophagic dysregulation. Traditional Chinese medicine has emerged as a promising strategy for managing respiratory inflammatory conditions. Ficus tikoua Bur. (FT), a well‑known ethnomedicinal prescription used for pneumonia in Guizhou, China, warrants investigation into its effects and mechanisms in ALI. This study integrated UHPLC‑Q‑TOF‑MS/MS chemical profiling, network pharmacology, molecular docking, and lipopolysaccharide‑challenged mouse model to characterize FT's bioactive constituents and therapeutic mechanisms. FT extract (FTE) significantly attenuated lung injury, reduced inflammatory cytokines (TNF‑α and IL‑6), and decreased oxidative stress markers (MPO and MDA). Network pharmacology and molecular docking identified stable interactions between core FT compounds and five key targets (mTOR, AKT1, PIK3CA, PIK3CB, and PIK3CD). Experimental validation further revealed that FTE inhibited PI3K/AKT overactivation, as evidenced by reduced p‑PI3K and AKT expression, while concurrently restoring autophagic homeostasis through downregulation of Beclin‑1 and LC3‑II/I and upregulation of p62. These findings demonstrate that FTE alleviates ALI through coordinated regulation of the PI3K/AKT pathway and autophagic homeostasis. Collectively, this study provides a mechanistic foundation for FT as a multi‑component therapeutic candidate against ALI and offers new perspectives for natural product‑based drug discovery. - Source: PubMed
Publication date: 2026/07/14
Li MengyuXu YitangYang MingyuJiang XingLiu YingxingLei LuxiLu XiangWan XiaoxiaYang Ye - Osteoporosis is a major public health concern characterized by reduced bone mass and increased fracture risk, and Cyathulae Radix has been used in China for its prevention, yet its active constituents and mechanisms remain unclear. This study integrated network pharmacology with in vitro experiments to identify key bioactive components and underlying pathways. Using the TCMSP database, we screened the major compounds of Cyathulae Radix and, through intersection with osteoporosis-related targets from OMIM and GeneCards, obtained 29 overlapping genes. Subsequent protein-protein interaction network analysis via STRING revealed core targets including AKT1, TNF, IL-6, and MMP2, among which quercetin emerged as a principal active component. To validate its effects, we employed H₂O₂ to induce oxidative damage in MC3T3 E1 pre-osteoblasts, and then treated cells with quercetin based on CCK8 and ROS assay results. The CCK8 assay showed that quercetin restored cell viability to near-normal levels. Quercetin treatment significantly improved cell proliferation and restored osteogenic differentiation, as shown by elevated alkaline phosphatase staining intensity, increased mineralized nodules in alizarin red staining, and markedly upregulated mRNA levels of RUNX2, OPN, and BMP-2. Furthermore, quercetin attenuated oxidative stress by increasing SOD and GSH activities, decreasing MDA production, and activating the NRF2/HO-1/GPX4 antioxidant cascade, as confirmed by western blotting. Collectively, these results demonstrate that quercetin, a key constituent of Cyathulae Radix, exerts anti osteoporotic effects through both promoting osteogenesis and enhancing antioxidant defense, offering a mechanistic rationale for its clinical use against osteoporosis. - Source: PubMed
Publication date: 2026/07/20
Li FeilongJiang YingcunZhou YangSong ChaoLiu ZongchaoFu Zhijiang - Colorectal cancer (CRC) is a prevalent gastrointestinal carcinoma. Huanglian decoction (HD) has been applied to treat gastrointestinal disorders for millennia. - Source: PubMed
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