AKT1 (phospho_Thr450) Antibody
- Known as:
- AKT1 (phospho_Thr450) Antibody
- Catalog number:
- E011502-2
- Product Quantity:
- 100ug
- Category:
- Antibodies
- Supplier:
- EnoGene
- Gene target:
- AKT1 (phospho_Thr450) Antibody
Related genes to: AKT1 (phospho_Thr450) Antibody
- Gene:
- AKT1 NIH gene
- Name:
- AKT serine/threonine kinase 1
- Previous symbol:
- -
- Synonyms:
- RAC, PKB, PRKBA, AKT
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: AKT1 (phospho_Thr450) Antibody
Related articles to: AKT1 (phospho_Thr450) Antibody
- Hepatic glycogen accumulation is a hallmark of glucose intolerance in carnivorous fish, yet the molecular mechanisms governing the partitioning of surplus carbohydrates remain poorly understood. This study integrated physiology and functional assays to elucidate how insulin-dependent mTOR/SREBP1 signaling governs hepatic glucose partitioning and glycogen accumulation. In the present study, we used largemouth bass (poor glucose utilization) and Nile tilapia (efficient glucose utilization) fed diets containing graded carbohydrate levels for 8 weeks. The results revealed that high-carbohydrate (HC) diets suppressed the PI3K/AKT1/mTOR axis in largemouth bass, reducing nuclear SREBP1 and causing massive hepatic glycogen accumulation. Conversely, tilapia efficiently activated this axis to promote lipid synthesis in response to excessive carbohydrates. Meanwhile, srebp1 knockdown in primary hepatocytes of largemouth bass decreased lipogenic gene expression and triglyceride content while increasing glycogen level. Mechanistic validations in largemouth bass demonstrated that insulin treatment restored AKT1/mTOR pathway activity and SREBP1 nuclear translocation, alleviating glycogen overload while promoting lipogenesis. Knockdown of akt1 or s6k1 prevented SREBP1 activation, whereas tsc2 knockdown rescued mTOR phosphorylation. Furthermore, mTOR inhibition by rapamycin abolished insulin-induced SREBP1 transactivation of lipogenic targets, mimicking the HC-induced glycogen-overload phenotype. In summary, this study identifies the insulin-responsive mTOR/SREBP1 signaling axis as the critical pathway governing lipogenesis in largemouth bass. Functional impairment of the insulin-responsive mTOR/SREBP1 signaling axis is associated with a metabolic shift favoring glycogen storage over lipogenesis, providing mechanistic insights relevant to glucose intolerance across vertebrates. - Source: PubMed
Tao JiajieChen ShiwenLiu NingGong YeZhang SenHe JiaxiongHuang XuxiongChen NaisongLi Songlin - Postpartum depression (PPD) is a prevalent psychological condition among birthing women. While several psycho-socio-economic and neurobiological factors influence its development, its relationship with smoking behavior and nicotine addiction remains largely inconclusive. - Source: PubMed
Publication date: 2026/08/17
Abedin FarheenaraDas MadhuparnaBishayi AratrikaSaha ParnaHusna AsmaulHaque ShafiulRana Sandeep SinghSudesh RaviAhmad Faraz - Guava ( L.) leaves are valuable functional ingredients; however, evidence linking environmental origin to metabolite-resolved bioactivity remains limited. This study investigated leaves from five Bolivian sites across two ecological regions. While the total phenolic content remained stable, the total flavonoid content and -glucosidase inhibition varied significantly across origins. Widely targeted LC-MS/MS annotated 1230 phenolic features and 753 differential metabolites, revealing an environmental-driven metabolic trade-off: high-irradiance lowlands accumulate lipophilic aglycones, whereas rainforests sequester hydrophilic flavonoid glycosides. Chemometrics revealed origin-dependent chemotypes: Amazonian extracts are glycoside-rich with strong enzymatic inhibition, whereas Lowland extracts are aglycone-enriched for radical scavenging. Network pharmacology and molecular docking prioritized norwogonin, predicting high TNF affinity (-10.1 kcal/mol) and AGE-RAGE axis targeting (e.g., AGER, AKT1, MAPK14). These predictions require experimental validation. Resolving the chemical heterogeneity of Bolivian guava, this work proposes a dual-chemotype classification, providing a robust framework for the strategic sourcing and standardization of functional food resources. - Source: PubMed
Publication date: 2026/08/03
Tao Xin-YuWu Zhou-WeiLiu Xiao-CuiQuan Chen-XiCabero Marco AntonioQiu Ming-Hua - Triple-negative breast cancer (TNBC) is an aggressive subtype that lacks ER, PR, and HER2, which results in limited treatment options and poor outcomes. Lichens are symbiotic organisms known for producing unique secondary metabolites and have a history of use in folk medicine. This study investigated the effects of lichen-derived compounds on MDA-MB-231 cells using integrated in vitro and in silico approaches to explore their anticancer potential and potential molecular targets. Six lichen compounds were isolated, characterized, and tested for antioxidant properties using DPPH and ABTS assays. In vitro cytotoxicity was evaluated on MDA-MB-231 using the MTT and FACS assays. ADMET was evaluated using SwissADME and ADMETLab3; GeneCards was used to retrieve the TNBC-associated genes. The STRING database is used to construct the protein-protein interaction network. Molecular docking analysis was done using CB Dock 2. Structural fluctuation was evaluated in CABS-flex 3.0 web server, and an open-source web-based platform, ShinyGO, was used for functional enrichment analysis. Out of six studied lichens, the extract of Everniastrum cirrhatum showed comparatively stronger antioxidant activity in terms of DPPH and ABTS radical scavenging potential. A decrease of up to 80% in cell viability at 320 µg/mL was observed when treated with Everniastrum cirrhatum, Heterodermia boryi, and Usnea longissima extracts. Six lichen compounds were isolated and characterized. ADMET analysis of isolated compounds suggests that they have acceptable drug-like characteristics with minimal major organ toxicity. The analysis of cytotoxicity using MTT and flow cytometry demonstrated a clear dose-dependent reduction in cell viability by atranorin (ATR), barbatic acid (BAR), and usnic acid (USN). Top 14 TNBC-associated hub genes were identified, and molecular docking analysis suggested that ATR and BAR have favorable binding affinities toward AKT1 and mTOR. RMSF analysis predicted reduced structural fluctuations in selected regions of AKT1 and mTOR following ligand binding, suggesting potential stabilization while preserving overall protein dynamics. KEGG pathway enrichment indicated that these hub genes are enriched in cancer-related pathways, including endometrial cancer and central carbon metabolism in cancer, and are associated with the PI3K/AKT/mTOR and RAS/MAPK signaling pathways. Lichen-derived compounds atranorin, barbatic acid, and usnic acid demonstrated anticancer activity in vitro, while integrated computational analyses suggested potential multitarget interactions that warrant further experimental validation. - Source: PubMed
Publication date: 2026/08/15
Mapari Sachin VGaikwad Subhash BSutar Ruchira RKhare RoshniPatil Ravindra MBehera Bhaskar C - Jianzhong Peiyuan Decoction, based on Shenling Baizhu Powder, is commonly used for stable COPD in Guizhou. However, its chemical composition and pharmacodynamics are not well understood. This study aims to explore its pharmacodynamic basis and multi-target pathways in COPD treatment and to confirm the intervention effect of its key active ingredient, luteolin, through in vitro cell experiments. - Source: PubMed
Publication date: 2026/08/10
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