SLC12A2 / NKCC1 antibody Ab host: Goat
- Known as:
- SLC12A2 / NKCC1 (anti-) Antibody production species: Goat
- Catalog number:
- 183446
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Acris antibodies
- Gene target:
- SLC12A2 / NKCC1 antibody host: Goat
Related genes to: SLC12A2 / NKCC1 antibody Ab host: Goat
- Gene:
- PPP1R18 NIH gene
- Name:
- protein phosphatase 1 regulatory subunit 18
- Previous symbol:
- KIAA1949
- Synonyms:
- phostensin
- Chromosome:
- 6p21.33
- Locus Type:
- gene with protein product
- Date approved:
- 2004-03-02
- Date modifiied:
- 2016-10-05
- Gene:
- SLC12A2 NIH gene
- Name:
- solute carrier family 12 member 2
- Previous symbol:
- -
- Synonyms:
- NKCC1, BSC, BSC2, PPP1R141
- Chromosome:
- 5q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-02-16
- Date modifiied:
- 2016-02-17
Related products to: SLC12A2 / NKCC1 antibody Ab host: Goat
Related articles to: SLC12A2 / NKCC1 antibody Ab host: Goat
- Solute carrier (SLC) transporters comprise a family of >450 membrane-bound proteins that facilitate the transport of a wide array of substrates across biological membranes. They play a fundamental role in controlling the transport of molecules, such as ions and metabolites, across the cell membranes. Despite the availability of marketed drugs targeting well-known SLC transporters, such as neurotransmitter (i.e. SERT, NET, DAT) and glucose (GLUTs) transporters, most of the SLCs are still under-investigated as therapeutic targets. A major limiting factor in this area is the lack of suitable assays and tools that enable High Throughput Screening (HTS) of large compound collections, aiming to identify novel therapeutics. In the context of the Innovative Medicines Initiative consortium RESOLUTE, we developed cell-based assays for several SLCs employing a variety of scientific approaches and technologies suitable for running fully automated HTS. Here, we describe the functional assays developed for five SLCs: SLC59A1 (MFSD2A), SLC59A2 (MFSD2B), SLC6A8 (CRTR), SLC9B2 (NHA2) and SLC12A2 (NKCC1). All these transporters play relevant roles in different pathological conditions, but they still lack drugs able to specifically activate or inhibit them. To address this gap, we have developed and optimized in a miniaturized format cellular assays that enable streamlined and high-throughput investigation of compound libraries. These assays offer a valuable platform for the identification of new therapeutic modulators targeting these underexplored SLCs in a fast and efficient manner. - Source: PubMed
Publication date: 2026/08/26
Sassone FrancescaKoch JosefinaBatoulis HelenaTremolada SaraRicci FernandaMaresca GiovannaEhrmann AlexanderScarabottolo Lia - Venous thromboembolism (VTE) includes deep vein thrombosis (DVT) and pulmonary embolism (PE), which often originate from DVT and can be fatal. - Source: PubMed
Publication date: 2026/07/27
Lozano-Esparza SusanaShakt Gabrielle EBrody Jennifer AMartinez-Perez AngelConomos Matthew PGermain MarineClapham Katharine RTeder-Laving Marisvan Hylckama Vlieg AstridKauko AnniThibord FlorianBezerra Ohanna C LNadkarni GirishMunsch GaëlleNøst Therese HaugdahlGoode Ellen LJi YuekaiChasman Daniel IReiner Alexander PTurman ConstanceWiggins Kerri LSitlani Colleen MSouto Juan CarlosLutsey Pamela LBellomo TiffanyBiobank EstonianProgram Million VeteranLi-Gao RuifangWinstén Aleksi KristianFinnGen Chen Ming-HueiDo RonGourhant LénaickHveem KristianArmasu Sebastian MSaut NoémiePankratz NathanGiulianini FrancoHaessler JeffreySong MingyangOlaso RobertBoerwinkle EricDochtermann DanielSoria Jose ManuelRich Stephen SSmadja DavidKoyama SatoshiRosendaal Frits RNiiranen TeemuGagnon FranceMy T Vy HaZakai NeilLemarie CatherineSkogholt Anne HeidiDeleuze Jean-FrançoisPankow James SRidker Paul MLiu YuxiRice Ken MPyarajan SaijuCushman MaryEmmerich JospehPsaty Bruce MNatarajan PradeepJohnson Andrew DRodger Marc ASuchon PierreCouturaud FrancisMorange Pierre-EmmanuelTang WeihongKooperberg CharlesKabrhel ChristopherSabater-Lleal MariaTrégouët David-AlexandreWolberg Alisa SDamrauer Scott MSmith Nicholas L - Prenatal ethanol (alcohol) exposure (PreEE) results in abnormal tangential migration of medial ganglionic eminence (MGE)-derived GABAergic interneurons (GINs) in the embryonic mouse medial prefrontal cortex (mPFC); later in life, this is associated with impaired behavioral flexibility commonly seen in fetal alcohol spectrum disorders (FASD). The PreEE-induced abnormal tangential migration involves, in part, ethanol potentiating GABA receptor-mediated depolarization, the latter due to elevated intracellular chloride sustained primarily by the Na-K-Cl cotransporter NKCC1 in embryonic GINs. We previously reported that coincident administration of the NKCC1 antagonist bumetanide with PreEE prevents the PreEE-induced aberrant migration of GINs in the mPFC. However, such simultaneous dispensing rarely occurs in real life. In this light, the present study assessed whether bumetanide administered after PreEE mitigates the PreEE-induced aberrant tangential migration. - Source: PubMed
Koc BetulSkorput Alexander G JYeh Pamela W LYeh Hermes H - During early postnatal development, γ-aminobutyric acid (GABA) signaling undergoes a functional switch from excitation to inhibition, driven by age-dependent shifts in intracellular chloride concentrations ([Cl⁻]). In the retina, starburst amacrine cells (SACs) are pivotal for establishing direction-selective circuitry. However, the molecular mechanisms and the precise timing of the reversal potential of GABA-induced currents (E) shift in mouse SACs remain to be fully elucidated. We investigated the maturation of GABA responsiveness and chloride homeostasis in mouse SACs during the neonatal period. Ca imaging in dissociated retinal neurons revealed that the application of GABA markedly increased intracellular Ca concentrations in SACs on postnatal day (P) 0-P2, whereas these excitatory responses were largely absent by P7-P9. Gramicidin-perforated patch recordings showed that E underwent a significant hyperpolarizing shift with development, accompanied by a marked decrease in [Cl⁻]. Immunohistochemical analyses demonstrated a developmental decline in Na-K-2Cl⁻ co-transporter (NKCC1) expression and the concomitant up-regulation of K-Cl⁻ co-transporter (KCC2) in SACs. Consistent with these expression patterns, the pharmacological inhibition of NKCC1 or KCC2 selectively changed E in a stage-dependent manner. Collectively, these results demonstrate a developmentally regulated GABAergic switch in SACs orchestrated by coordinated changes in chloride transporter expression, providing insights into the physiological maturation of the retinal circuitry underlying direction selectivity. - Source: PubMed
Publication date: 2026/07/03
Ishii ToshiyukiYin ChengzhuWatanabe KazuakiKaneda MakotoKato Daisuke - The mammalian tracheal epithelium is composed of different cell types distributed along the proximal-distal axis. Nevertheless, variations in expression and function of ion channels and transporters participating in fluid absorption and secretion have never been studied separately in proximal and distal mouse trachea. This work aims to characterize basal and stimulated absorption and secretion of fluid obtained from proximal and distal trachea from the same animal. - Source: PubMed
Apablaza TábataVillanueva SandraOlave-Ruiz AraceliGuequen AnitaCatalán Marcelo AFlores Carlos A