MGLL Antibody
- Known as:
- MGLL Antibody
- Catalog number:
- XW-7936
- Product Quantity:
- 0.05 mg
- Category:
- -
- Supplier:
- Prosci
- Gene target:
- MGLL Antibody
Related genes to: MGLL Antibody
- Gene:
- MGLL NIH gene
- Name:
- monoglyceride lipase
- Previous symbol:
- -
- Synonyms:
- HU-K5, MGL
- Chromosome:
- 3q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-13
- Date modifiied:
- 2016-10-05
Related products to: MGLL Antibody
Related articles to: MGLL Antibody
- Thyroid hormones are central regulators of metabolic homeostasis and developmental programming. The active hormone triiodothyronine (T3) modulates transcription through nuclear receptors that recruit epigenetic cofactors to remodel chromatin and regulate metabolic gene networks. Although thyroid hormone signaling is known to influence lipid metabolism, whether it coordinates lipid droplet turnover with autophagy-related pathways during early embryonic development remains largely unknown. Here, transcriptomic profiling revealed distinct metabolic signatures between and embryos, with marked differences in fatty acid metabolism. Supplementation with 50 nM T3 enhanced blastocyst formation, particularly when applied from the 4-cell to blastocyst stages, coinciding with elevated thyroid hormone receptor expression. T3 induced robust lipid droplet remodeling, characterized by reduced droplet size, together with increased lipid-mitochondria colocalization and activation of lysosomal and mitochondrial pathways, consistent with enhanced lipid catabolism and organelle coupling. Mechanistically, inhibition of the histone acetyltransferase KAT2B/PCAF abolished T3-mediated developmental gains, reduced H3K9ac and H3K27ac, and resulted in nonselective autophagic stress rather than lipophagy. By contrast, T3 required KAT2B to stimulate cytosolic lipolysis, channel fatty acids into mitochondria, and enhance mitochondrial membrane potential. T3 also upregulated prostaglandin biosynthesis genes and improved outgrowth performance. These findings identify a thyroid hormone-KAT2B epigenetic axis that coordinates lipid droplet remodeling through lipolytic and lipophagic pathways, linking endocrine signaling to organelle crosstalk and mitochondrial activation during early embryogenesis. ART: assisted reproductive technology; ATG5: autophagy related 5; BSA: bovine serum albumin; BSCL2/SEIPIN: BSCL2 lipid droplet biogenesis associated, seipin; CARM1: coactivator associated arginine methyltransferase 1; COCs: cumulus-oocyte complexes; CPT1A: carnitine palmitoyltransferase 1A; CPT2: carnitine palmitoyltransferase 2; CREBBP/CBP: CREB binding protein; DEGs: differentially expressed genes; DGAT1: diacylglycerol O-acyltransferase 1; EP300: E1A binding protein p300; ER: endoplasmic reticulum; H3K9ac: histone H3 acetyl-Lys9; H3K27ac: histone H3 acetyl-Lys27; HCS: high-content screening; HDACs: histone deacetylases; IVC: in vitro culture; IVF: in vitro-fertilized embryos; IVM: in vitro maturation; IVO: in vivo embryos; KAT2A/GCN5: K(lysine) acetyltransferase 2A; KAT2B/PCAF: K(lysine) acetyltransferase 2B; KAT2Bi (Ki): KAT2B inhibition; KD: knockdown; LC3: microtubule associated protein 1 light chain 3; LDs: lipid droplets; LIPE/HSL: lipase E, hormone sensitive type; MGLL: monoglyceride lipase; MMP: mitochondrial membrane potential; mtDNA: mitochondrial DNA; MT-ND1: mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1; PA: parthenogenetically activated; PG: prostaglandin; PLA2G4A: phospholipase A2 group IVA; PLIN2: perilipin 2; PLIN3: perilipin 3; PLIN5: perilipin 5; PNPLA2/ATGL: patatin like phospholipase domain containing 2; PPARD/PPARδ: peroxisome proliferator activated receptor delta; PPARs: peroxisome proliferator activated receptors; PRMT1: protein arginine methyltransferase 1; PTGS1: prostaglandin-endoperoxide synthase 1; PTGS2: prostaglandin-endoperoxide synthase 2; PVA: polyvinyl alcohol; RT: room temperature; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; RXR: retinoid X receptor; SIRT1: sirtuin 1; SLC25A20/CACT: solute carrier family 25 member 20; SLC27A4/FATP4: solute carrier family 27 member 4; SUV39H1: SUV39H1 histone lysine methyltransferase; T3: triiodothyronine; T4: thyroxine; TAGs: triacylglycerols; TEM: transmission electron microscopy; THR: thyroid hormone receptor; THRA: thyroid hormone receptor alpha; THRA-i: thyroid hormone receptor antagonist; THRB: thyroid hormone receptor beta; THs: thyroid hormones; ZGA: zygotic genome activation. - Source: PubMed
Publication date: 2026/09/24
Lee Song-HeeZhan Cheng-LinCui Xiang-Shun - Meat quality is an important economic trait in beef cattle and is influenced by breed-related metabolic characteristics. Yanbian cattle (YB) are valued for desirable meat quality, whereas Yanhuang cattle (YH), developed using Limousin cattle as the paternal line and Yanbian cattle as the maternal line, exhibit improved growth performance and carcass yield. However, the molecular basis underlying metabolic variation between these two genetically related cattle populations remains unclear. In this study, longissimus thoracis muscle samples from six animals per breed were analyzed using LC-MS/MS-based metabolomics and GC×GC-TOF/MS-based volatile compound profiling, and a subset of three samples per breed from the same cohort was selected for transcriptomic sequencing. A total of 1697 metabolites were detected. Based on the screening criteria of VIP > 1 and < 0.05, 202 candidate metabolites were identified, among which 11 remained statistically significant after false discovery rate (FDR) correction. Volatile compound profiling detected 1333 and 1516 compounds in YB and YH, respectively, of which 809 were shared. Based on the same screening criteria, 39 candidate volatile compounds were identified, although none remained significant after FDR correction. Transcriptomic analysis identified 360 differentially expressed genes using |logFC| > 1 and adjusted < 0.05, with enrichment mainly observed in pathways associated with carbohydrate metabolism, lipid turnover, and energy utilization. Integrative analyses indicated that amino acid-related metabolites, including phenylpyruvate, 2-aminobenzoic acid, and asparagine, were more closely associated with candidate volatile compounds than metabolites involved in central carbon metabolism. Several genes involved in lipid metabolism, energy metabolism, and muscle structure, including , , , , , , , and , were associated with distinct metabolic modules. These findings provide an exploratory view of breed-related metabolic variation and identify candidate molecular features for future validation. - Source: PubMed
Publication date: 2026/08/10
Lyu YangZhu ZhiweiZhao BaoxinRen ZezhuZhou MengYu JingDing HeLiu HongyuFang YiZhao JingLyu Wenfa - Lamivudine and abacavir are key components of first-line paediatric antiretroviral therapy (ART). Rifampicin is part of drug-sensitive TB treatment in children. Rifampicin induces UDP-glucuronosyltransferases and renal transporters and inhibits gastrointestinal transporters involved in the pharmacokinetics of abacavir and lamivudine, but pharmacokinetic data on this potential interaction in infants are scarce. - Source: PubMed
Beca Laize Sílvia Dos Anjos BotasMumbiro VivianBwakura MutsaMujuru Hilda AMudzingwa ShepherdTagarro AlfredoDomínguez-Rodríguez SaraMusiime VictorNalwanga DamalieBuck W ChrisSacarlal JahitChabala ChishalaChansa Bwendo NdunaRojo PabloBurger David MMoraleda CintaJacobs Tom G - Osteoarthritis (OA) is a chronic, progressive joint disorder with higher prevalence and pain severity in women than men. The endocannabinoid system (ECS) modulates pain and shows sexual dimorphism, yet sex-specific alterations in central ECS signalling in OA pain remain under-investigated. - Source: PubMed
Publication date: 2026/07/19
Ferdousi Mehnaz IInfantino RosmaraRedmond Maria CKrishnan Santhosh DAdjei Canny KMcDermott BarryLiddy AlisonQuinlan LeoO'Halloran MartinFinn David P - The aim of this study was to investigate the effects of cannabidiol (CBD) on memory deficits induced by ovariectomy and to directly compare its effects with those of hormone therapy, in order to better understand potential shared mechanisms related to menopause-associated cognitive decline, with a particular focus on the endocannabinoid system. Three-month-old female Wistar rats were randomly assigned to four experimental groups: SHAM-Veh (Vehicle), OVX-Veh, OVX-E2 (estradiol), and OVX-CBD. Animals underwent either bilateral ovariectomy or sham surgery. Following a three-week recovery period, rats received daily subcutaneous injections of CBD (10 mg/kg), estradiol (10 μg/kg), or vehicle for 21 consecutive days. Behavioral assessments included object recognition and fear-motivated memory tests. Twenty-four hours after the final treatment, animals were euthanized for neurochemical and molecular analyses. Levels of the endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were measured using high-performance liquid chromatography. Gene expression of cannabinoid receptors CB1 and CB2, as well as enzymes involved in the synthesis and degradation of endocannabinoids (NAPE-PLD, DAGL-A, FAAH, and MGLL), was evaluated in the hippocampus by RT-qPCR. The results demonstrated that CBD treatment produced memory improvements in the object recognition task comparable to those observed with estradiol. Ovariectomy-induced impairments in fear-motivated memory were completely reversed by both CBD and estradiol treatments. Additionally, CBD reduced the expression of FAAH and MGLL, resulting in increased hippocampal levels of AEA and 2-AG, effects similar to those observed with hormone therapy. Estradiol also increased NAPE-PLD expression, contributing to elevated AEA levels. Overall, the findings suggest that CBD exerts a protective effect on memory comparable to standard estrogen therapy, supporting its therapeutic potential for menopause-related cognitive impairments. - Source: PubMed
Caruso Fernanda BorsattoSevero Maria Paula ArakakiColucci Anna Clara MachadoVuaden Beatriz Cristina WeidleKowalski LayzaSchonhofen PatríciaFranke Felipe Schroederde Mello Fernanda Bastosda Silva Rodrigues FernandaGuedes Renata Padilhade Lima Maria Noêmia MartinsHallak Jaime E CZuardi Antônio WaldoCrippa José Alexandre SSchröder Nadja