RGS11 Antibody
- Known as:
- RGS11 Antibody
- Catalog number:
- XW-7521
- Product Quantity:
- 0.05 mg
- Category:
- -
- Supplier:
- Prosci
- Gene target:
- RGS11 Antibody
Related genes to: RGS11 Antibody
- Gene:
- RGS11 NIH gene
- Name:
- regulator of G protein signaling 11
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 16p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1998-12-15
- Date modifiied:
- 2017-04-13
Related products to: RGS11 Antibody
Related articles to: RGS11 Antibody
- Melanopsin, a G-protein coupled receptor (GPCR) and photopigment, mediates nonimage-forming and image-forming visual behaviors. There are six subtypes of intrinsically photosensitive retinal ganglion cells (ipRGCs) in the mouse retina; although melanopsin signals through Gα in all subtypes, evidence suggests that M1 and M4 ipRGCs may utilize distinct phototransduction pathways. We sought to uncover a mechanism that might enable a single GPCR to initiate different signaling cascades in these ipRGC subtypes. In HEK293 cells heterologously expressing mouse melanopsin, we show that melanopsin couples promiscuously to all four G-protein families and generates cAMP via a Gα-Gβγ-dependent mechanism. In the mouse retina, activation of virally expressed DREADD-Gs demonstrated that cAMP signaling drives action potentials in both M1 and M4 ipRGCs, revealing that M1 cells possess the adenylate cyclase and cAMP-sensitive channels required to respond to cAMP. Using a meta-analysis of single-cell transcriptomics together with RNAscope in the retina, we discovered that ipRGC subtypes differ and selectively enrich certain regulators of G-protein signaling (RGS). Rgs16 is enriched in a subset of M1 ipRGCs, whereas M4 ipRGCs preferentially express Rgs5 and Rgs11. The G-protein selectivity of these RGS proteins under heterologous expression revealed that RGS5 is selective for Gα, and a combination of RGS proteins found in M4 ipRGCs proteins severely attenuates Gα signaling compared with M1-like RGS. Our heterologous expression data together with experiments in native ipRGCs suggest a model where the subtype-specific repertoire of RGS proteins in M4 ipRGCs might attenuate Gα signaling, permitting enhanced Gα-Gβγ-driven cAMP. This study provides a potential mechanistic explanation of how a single GPCR can initiate distinct phototransduction cascades in separate cell types. - Source: PubMed
Publication date: 2025/11/07
Harkless Lee HDaly K MOstermeyer Grayson PHattar SamerBrown R LaneRobinson Phyllis R - Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy associated with early metastasis, drug resistance, and poor outcomes. We previously demonstrated a putative tumor-suppressive role for concentrative nucleoside transporter 1 (CNT1) in PDAC. In this study, we demonstrate the regulator of G protein signaling (RGS) 11 as a key target of CNT1, with potent tumor-suppressive properties in PDAC. Compared with normal human pancreas, RGS11 expression is diminished in human PDAC tissues which correspond with the reduced patient survival times. In addition, quasimesenchymal pancreatic tumor cell lines with accelerated growth, metastatic propensity, and innate resistance to nucleoside analogs showed relatively lower RGS11 expression than their epithelial counterparts. Interestingly, RGS11 levels reversibly modulated the epithelial-mesenchymal transition of human PDAC cell lines influencing the chemotherapeutic sensitivities of anti-PDAC drugs. Additionally, stable lentiviral-mediated RGS11 expression reduced the cellular proliferation and colony establishment, increased the apoptotic index, and decreased the migratory and invasive abilities in quasimesenchymal tumor cell lines, whereas RGS11 depletion in epithelial tumor cell lines showed opposite effects. Global transcriptomic analysis revealed RGS11 replenishment in PDAC cells to suppress CD44-directed stemness features with significant reprogramming of the PDAC oncogenic landscape. Furthermore, RGS11 reduced the primary tumor burden and metastatic occurrence in a mouse model of PDAC. Together, these findings uncover RGS11 as a key target of CNT1 that exhibits therapeutic potential for intervention of aggressive PDAC. - Source: PubMed
Kaur TejinderNayak DebasisJoshi ArnavLi JunanHite AmyGovindarajan Rajgopal - Atrial fibrillation (AF) is a common diagnosed heart disease that needs novel managements. This study aimed to seek potential biomarkers and underlying regulatory pathways associated with AF. - Source: PubMed
Publication date: 2025/03/13
Huang HaoXiong YanZeng Jie - To describe a novel α-thalassemiadeletion identified from a newborn by third-generation sequencing (TGS). - Source: PubMed
Publication date: 2024/10/09
Guo JingLi TengLiang LiangWei WeiLi YanGuo WeilinLi Youqiong - The ability of the visual system to relay meaningful information over a wide range of lighting conditions is critical to functional vision, and relies on mechanisms of adaptation within the retina that adjust sensitivity and gain as ambient light changes. Photoreceptor synapses represent the first stage of image processing in the visual system, thus activity-driven changes at this site are a potentially powerful, yet under-studied means of adaptation. To gain insight into these mechanisms, the abundance and distribution of key synaptic proteins involved in photoreceptor to ON-bipolar cell transmission were compared between light-adapted mice and mice subjected to prolonged dark exposure (72 hours), by immunofluorescence confocal microscopy and immunoblotting. We also tested the effects on protein abundance and distribution of 0.5-4 hours of light exposure following prolonged darkness. Proteins examined included the synaptic ribbon protein, ribeye, and components of the ON-bipolar cell signal transduction pathway (mGluR6, TRPM1, RGS11, GPR179, Goα). The results indicate a reduction in immunoreactivity for ribeye, TRPM1, mGluR6, and RGS11 following prolonged dark exposure compared to the light-adapted state, but a rapid restoration of the light-adapted pattern upon light exposure. Electron microscopy revealed similar ultrastructure of light-adapted and dark-adapted photoreceptor terminals, with the exception of electron dense vesicles in dark-adapted but not light-adapted ON-bipolar cell dendrites. To assess synaptic transmission from photoreceptors to ON-bipolar cells, we recorded electroretinograms after different dark exposure times (2, 16, 24, 48, 72 hours) and measured the b-wave to a-wave ratios. Consistent with the reduction in synaptic proteins, the b/a ratios were smaller following prolonged dark exposure (48-72 hours) compared to 16 hours dark exposure (13-21%, depending on flash intensity). Overall, the results provide evidence of light/dark-dependent plasticity in photoreceptor synapses at the biochemical, morphological, and physiological levels. - Source: PubMed
Publication date: 2023/10/03
Haley Tammie LHecht Ryan MRen GaoyingCarroll James RAicher Sue ADuvoisin Robert MMorgans Catherine W