AKT2 Antibody
- Known as:
- AKT2 Antibody
- Catalog number:
- 48-432
- Product Quantity:
- 0.05 mg
- Category:
- -
- Supplier:
- Prosci
- Gene target:
- AKT2 Antibody
Related genes to: AKT2 Antibody
- Gene:
- AKT2 NIH gene
- Name:
- AKT serine/threonine kinase 2
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 19q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-05
- Date modifiied:
- 2016-10-05
Related products to: AKT2 Antibody
Related articles to: AKT2 Antibody
- The oil extracted from the mesocarp of oil palm (Elaeis guineensis, Jacq.) drupes is the world's leading source of vegetable oil. Oil palm cultivation requires large amounts of mineral nutrients supplied through fertilization, representing a major production cost. Among essential nutrients, potassium (K⁺) plays a critical role in plant growth and fruit development. However, the mechanisms underlying K⁺ transport and its relationship with yield remain poorly understood. Here, we identified and characterized EgKT2-1, a Shaker-like potassium channel belonging to the AKT2-like subfamily. Quantitative PCR analyses showed that EgKT2-1 is expressed in leaves, meristem, and during early fruit development, with higher transcript abundance during early fruit development coinciding with increased K⁺ accumulation. In situ hybridization localized EgKT2-1 transcripts to the root cortex and phloem tissues, leaf palisade parenchyma, and mesocarp cells during early fruit development. These expression patterns are consistent with a potential contribution of EgKT2-1 to K⁺ transport and allocation between source and sink tissues. Although sequence similarity places EgKT2-1 within the weakly rectifying AKT2-like subfamily, electrophysiological characterization in Xenopus laevis oocytes revealed inwardly rectifying channel activity, suggesting a potential role in K⁺ uptake. EgKT2-1 expression was higher in an oil palm progeny C3 which exhibited greater K⁺ allocation to aerial organs and greater oil yield. Together, these findings identify EgKT2-1 as an inwardly rectifying K⁺ channel potentially involved in K⁺ transport and redistribution during oil palm development and suggest a correlation between EgKT2-1 expression, K⁺ allocation, and oil yield variation. - Source: PubMed
Publication date: 2026/09/30
Monder HousseinEspeout SandraZimmermann Sabine DBillotte NorbertVerdeil Jean-LucBocs StéphanieOllivier JeanImpens ReinoutJacob FlorenceGaillard IsabelleCuéllar Teresa - Akt is a central serine/threonine kinase in the PI3K/Akt/mTOR pathway that regulates cell survival, growth, and metabolic homeostasis. Although Akt1, Akt2, and Akt3 share high sequence homology, they perform distinct physiological and pathological functions, motivating the development of small-molecule isoform-selective chemical probes. Here, we first applied a molecular hybridization strategy to combine structural features that lead to Akt3-selectivity with the covalent pharmacophore of established covalent-allosteric Akt inhibitors, enabling selective and covalent targeting of Akt3. We then expanded the chemical space of covalent-allosteric Akt inhibitors to investigate how subtle structural differences influence selectivity across all three isoforms. Guided by structure-activity relationships and high-resolution co-crystal structures, systematic modifications of substituent patterns, linker geometry, and scaffold architecture revealed distinct isoform-selectivity profiles. Biochemical target inhibition, cellular target engagement, and covalent-binding studies further characterized these compounds and their selectivity. Collectively, these findings expand the covalent-allosteric Akt inhibitor toolbox, provide insight into the structural determinants governing Akt isoform selectivity, and establish molecular hybridization as a strategy for selective covalent targeting of Akt3. - Source: PubMed
Publication date: 2026/09/29
Pervanidis Kosmas AlexandrosD'Angelo Giovanni DaniloAthanasiadis IoannisJantzen HannahScrima AndreaSchäfer KatharinaQuambusch LenaMüller Matthias PRauh Daniel - Oliv. cv. Chaxiong (Chaxiong) has traditionally been used as a medicinal tea for headache relief in southern China, but its bioactive basis and mechanisms remain unclear. This study integrated ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS), untargeted metabolomics, network pharmacology, molecular docking, reverse-transcription quantitative PCR (RT-qPCR), and Western blotting in a nitroglycerin-induced migraine-like rat model. Chaxiong extract alleviated migraine-like behavior, reduced calcitonin gene-related peptide (CGRP), nitric oxide (NO), and tumor necrosis factor-α (TNF-α), increased 5-hydroxytryptamine (5-HT), and ameliorated brain histopathological alterations. A total of 48 constituents and 13 differential serum metabolites were annotated or putatively annotated, respectively. Glycerophospholipid metabolism was the principal metabolic pathway affected by treatment, whereas network pharmacology prioritized phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling. Pathway integration identified PIK3CA, PIK3CB, AKT1, AKT2, and INS as candidate bridging targets. Exploratory docking suggested potential interactions between five representative constituents and PIK3CA/AKT1. RT-qPCR showed reduced and mRNA expression, while Western blotting showed decreased p-PI3K p85α (Tyr607)/PI3K p85α and p-AKT (Ser473)/AKT1 ratios following treatment. These findings suggest that Chaxiong-associated alleviation of migraine-like responses was accompanied by altered glycerophospholipid metabolism and reduced PI3K/AKT pathway activation. - Source: PubMed
Publication date: 2026/09/14
Zhu YuDong YanjingJiang ShiyiQin QianHe YuWu DanyangZhang ShouwenWei Juan - : An analysis of microRNA (miRNA) expression signatures in lung adenocarcinoma (LUAD) harboring mutations revealed that multiple miRNAs, including , were suppressed in cancer tissues. This study focused on and aimed to identify therapeutic target genes regulated by in LUAD harboring mutations and evaluate their potential for combination therapy with EGFR tyrosine kinase inhibitors. : The antitumor function of was analyzed by the ectopic expression of into LUAD cells with mutation. The target genes of were identified through an analysis of the TargetScan, Genecodis 4 and TCGA databases. The Chou-Talalay method was used to determine the potential synergistic effects of selected drug combinations. : The expression of attenuated the malignant transformation of LUAD cells through targeting the "EGFR tyrosine kinase inhibitor resistance pathway". We focused on among the genes involved in this pathway. Capivasertib was recently approved as the first inhibitor of oncogenic signaling. Therefore, we investigated the effect of combination therapy comprising osimertinib and capivasertib on LUAD cell viability. Combination therapy synergistically reduced cell viability in both PC9 and H1975 cells, with combination index values of 0.61 and 0.14, respectively, at Fa = 0.5. : Our miRNA-based analysis is an excellent strategy for identifying therapeutic targets that enhance the effects of EGFR inhibitors on LUAD. - Source: PubMed
Publication date: 2026/08/27
Tokunaga TakuyaIshihara YukaTakeda Aya HaradaTomioka YuyaNagata AyakoKato MayukoSuetsugu TakayukiMizuno KeikoUeda KazuhiroSeki Naohiko - : Unexplained infertility (UI) is defined by the absence of detectable abnormality on standard evaluation, which raises the possibility that the causative defect lies at the level of intracellular signaling rather than at the level of gamete number or hormone concentration. The PI3K/Akt pathway governs primordial follicle activation, granulosa cell survival, and the acquisition of oocyte competence, but AKT2 transcript abundance in the somatic follicular compartment has not been characterized clinically in UI. We compared cumulus/granulosa cell AKT2 transcript levels and follicular fluid Akt and phospho-Akt (Ser473) protein levels between women with UI and women undergoing intracytoplasmic sperm injection (ICSI) for isolated male factor infertility. : This was a prospective case-control study of 128 women (64 UI, 64 male factor) aged 23-35 years, all stimulated with a GnRH antagonist protocol and recruited December 2020-May 2021. AKT2 expression in cumulus/granulosa cells recovered at denudation was quantified using RT-qPCR (ΔΔCt, GAPDH reference). Total Akt and phospho-Akt (Ser473) were measured in follicular fluid using ELISA and normalized to the number of oocytes retrieved. Oocyte maturation, fertilization, day 3 embryo quality, and day 5 blastocyst quality were assessed using Alpha/ESHRE Istanbul consensus criteria. Correlation analyses were exploratory and reported with Benjamini-Hochberg false discovery rate (FDR) adjustment. : Baseline characteristics, including age, body mass index, antral follicle count, AMH, FSH, LH, oocytes retrieved, and duration of infertility, were comparable between groups. Granulosa cell AKT2 ΔCt was higher in the UI group (3.46 ± 1.47 versus 2.68 ± 1.18; = 0.001), corresponding to a 1.72-fold lower AKT2 transcript level (ΔΔCt 0.78; Cohen's d = 0.59); the difference persisted after adjustment for age, body mass index, AMH, basal estradiol levels, and previous IVF attempts (adjusted ΔCt difference 0.74, 95% CI 0.25-1.24; = 0.004). Follicular fluid total Akt (11.55 ± 4.08 versus 11.75 ± 3.60 relative units; = 0.78) and phospho-Akt (21.05 ± 7.14 versus 19.81 ± 7.24; = 0.34) did not differ, nor did values normalized per oocyte. Oocyte maturation rate (0.72 ± 0.25 versus 0.76 ± 0.18), fertilization rate (44.6 ± 29.5% versus 42.2 ± 25.6%), day 3 good-quality embryo rate (25.0 ± 25.8% versus 26.6 ± 23.8%), and good-quality blastocyst rate (30.2 ± 25.8% versus 20.5 ± 18.5%) were all comparable. Of 24 exploratory correlations, only two survived FDR adjustment. Both correlations were in the male factor comparison group and both involved the small blastocyst subgroup: total Akt per oocyte (ρ = 0.738) and phospho-Akt per oocyte (ρ = 0.728) versus good-quality blastocyst rate ( = 17; FDR = 0.011). AKT2 ΔCt did not correlate with any embryological endpoint in either group. : Cumulus and granulosa cell AKT2 mRNA was reduced approximately 1.7-fold in women with unexplained infertility, despite indistinguishable ovarian reserve, ovarian response, and embryological performance and despite unchanged follicular fluid Akt protein. Because the comparison group consisted of women from couples undergoing ICSI for male factor infertility rather than women of proven fertility, the difference cannot be attributed specifically to unexplained infertility, and the findings describe transcript abundance rather than pathway activity. Confirmation against a fertile comparison group, with protein-level assessment in the same cells and clinical pregnancy endpoints, is required. - Source: PubMed
Publication date: 2026/09/18
Uzun ÖzgürDevranoğlu BelginÖzkaya EnisDemirci HuriTunalı GüldenSezer ZehraSağ Fatma BeyzaGüzel Meydanlı Emine Elif