By moving chloride into epithelial cells, the Na-K-Cl cotransporter SLC12A2 aids transcellular movement of chloride across both secretory and absorptive epithelia.By moving chloride into epithelial ce
- Known as:
- By moving chloride epithelial cells, Na-K-Cl cotransporter SLC12A2 aids transcellular movement chloride secretory absorptive epithelia.By moving chloride epithelial ce
- Catalog number:
- 29-906
- Product Quantity:
- 0.1 mg
- Category:
- -
- Supplier:
- Prosci
- Gene target:
- moving chloride into epithelial cells the Na-K- cotransporter SLC12A2 aids transcellular movement across both secretory and absorptive epithelia.
Related genes to: By moving chloride into epithelial cells, the Na-K-Cl cotransporter SLC12A2 aids transcellular movement of chloride across both secretory and absorptive epithelia.By moving chloride into epithelial ce
- Gene:
- SLC12A2 NIH gene
- Name:
- solute carrier family 12 member 2
- Previous symbol:
- -
- Synonyms:
- NKCC1, BSC, BSC2, PPP1R141
- Chromosome:
- 5q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-02-16
- Date modifiied:
- 2016-02-17
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- Hepatocellular carcinoma (HCC) remains a major challenge threatening global health. It has a high incidence with poor prognosis, and its response to current conventional therapies is not satisfactory, so we need to explore new effective therapies to address this problem. Targeted therapy represents a promising approach, but the therapeutic efficacy of targeting ion transporters and nuclear transport proteins, such as Na+/K+/2Cl- cotransporter 1 (NKCC1) and Karyopherin β1 (KPNβ1), remains poorly investigated. This study aims to assess the therapeutic efficacy of targeting NKCC1 and KPNβ1 individually and in combination. We also explored the roles of these transporters in tumor cell proliferation and HCC progression. To achieve this aim, an HCC rat model was used, induced by diethylnitrosamine and phenobarbital. The evaluation included histopathological examination and assessment of liver function biomarkers (alanine aminotransferase and aspartate aminotransferase), cell cycle regulators (Cyclin D1, p21, E2F1), proliferation (Ki-67), apoptosis (Caspase-3), and expression of NKCC1 and KPNβ1. This study revealed that NKCC1 and KPNβ1 were upregulated in the HCC group. This increase was associated with increased proliferation and suppressed apoptosis. Targeting these proteins, particularly in combination, significantly improved liver function. It also induced cell cycle arrest, reduced proliferative markers, and enhanced apoptosis. These findings indicate that NKCC1 and KPNβ1 could serve as possible therapeutic targets in HCC treatment. - Source: PubMed
Abass Shimaa AElhasanein Doaa HEldomany Ramadan AZakaria Sherin - Tilapia species differ markedly in salinity tolerance, providing a useful system for investigating the molecular basis of osmotic adaptation. The solute carrier family 12 () encodes cation-chloride cotransporters that play central roles in ion homeostasis and osmoregulation. Here, we compared the SLC12 gene family among Nile tilapia (), Mozambique tilapia (), and blue tilapia () using comparative genomic, phylogenetic, protein-structural, and chromosomal analyses. Separately, we re-analyzed a published long-term salinity RNA-seq dataset from GIFT Nile tilapia maintained for 3.5 months at 0, 17, or 27 ppt, focusing on expression in the gill, intestine, and skin. All three tilapia species contained 17 loci with identical subfamily copy numbers and broadly conserved chromosomal distributions. In the Nile tilapia transcriptomic dataset, paralogous , and copies displayed distinct tissue- and salinity-dependent expression patterns. also showed salinity-responsive expression in Nile tilapia, prompting further comparative sequence and structural analysis. The three SLC12A8 orthologs were broadly similar in sequence and predicted structure, although several species-specific residue differences were observed. These findings reveal overall conservation of the family among tilapias, identify salinity-responsive expression differences among paralogous members in Nile tilapia and interspecific sequence variation in , and provide candidate features for further functional investigation. - Source: PubMed
Publication date: 2026/09/12
Pan Jin-MinLi Liu-YangDong Meng-FanChen Qian-HuiXia Jun-Hong - Hereditary hearing loss is a genetically heterogeneous disorder posing challenges for molecular diagnosis. This study aimed to characterize the mutation spectrum, including rare and previously unreported variants, using a tiered strategy in a Han Chinese cohort from Southwest China. - Source: PubMed
Zhang YuYuan ShimeiWan YafangLi TianXu LanlanYang WeiNiu Changchun - The Hilsa Shad is an anadromous fish of major ecological and economic importance in Bangladesh. It migrates between marine and freshwater environments and faces substantial osmotic challenges. There is limited research on osmoregulatory gene expression during migration across diverse habitats. This study provides the first genome-wide identification of the Slc12a2 gene in Hilsa Shad and confirms the existence of two Slc12a2 gene paralogs. To evaluate their potential role in salinity adaptation, we further examined their expression across contrasting habitats. The Bay of Bengal where the fish originates, the genome-wide expression of Slc12a2 has not yet been investigated. The Surma River has been identified as a significant freshwater habitat for Hilsa Shad. However, the molecular information including gene expression data has not yet been reported for this population. In this study, RT-qPCR was used to analyze gill, kidney, liver, and muscle tissues of Hilsa Shad. The results showed that both Slc12a2 paralogs were significantly down-regulated during upstream migration from the Bay to Bengal to the Surma River. These findings demonstrate the essential role of Slc12a2 in maintaining osmotic balance during habitat transition. This genome-wide approach also gives an overall picture of how multiple Slc12a2 gene copies respond to environmental salinity changes and regulate homeostasis. - Source: PubMed
Publication date: 2026/06/03
Aunkor Md Toasin HossainAlam Md JahangirZidan Musfiq AhamedKhan Mohammad Mehedi HasanKabir Muhammad AnamulMiah Md Faruque - Cajal-Retzius neurons (CRN) represent an early-born transient neuronal population in the mammalian neocortex. They are best known for secreting reelin, which is essential for neuronal migration and layer formation in the developing cortex. However, their functional integration into developing cortical circuits and their contribution to early network dynamics have been poorly understood. In this study, we investigated the structural and functional integration of CRN into developing neocortical networks with immunohistochemistry, three-dimensional reconstruction of GABAergic inputs, extracellular electrophysiology and calcium imaging. In addition, multiplexed FISH was used to determine the expression levels of the chloride transporters NKCC1 and KCC2 in CRN at different postnatal stages. Finally, we employed optogenetic stimulation to assess the functional and developmental consequences of CRN activation. We found that CRN receive dense dendritic GABAergic synaptic inputs at early postnatal stages. Functional analyses revealed that a substantial fraction of CRN is spontaneously active, and that their activity is synchronized with both CRN and non-CRN neurons. At the molecular level, they display a persistent NKCC1-dominant expression profile. This profile can contribute to the excitatory GABA responses, but also affect their cell death, as chronic blockade of NKCC1 was associated with reduced CRN loss in vitro. Acute optogenetic activation of CRN at early stages increased network excitability in organotypic neocortical cultures and induced stimulus-evoked field responses, while chronic activation at later time points accelerated CRN loss. Overall, these findings demonstrate that, during development, dense GABAergic input connectivity, coupled with the putative excitatory action of GABA, can engage CRN in early network activity. Furthermore, not only do CRN actively participate in early immature circuits, but their activity also amplifies spontaneous network dynamics and contributes to their own transience, highlighting a potential novel functional role of CRN during neocortical development. - Source: PubMed
Publication date: 2026/09/09
De Rosa FedericoKilb WernerLuhmann Heiko JSinning Anne