BLUE PROTEIN STAIN
- Known as:
- BLUE PROTEIN STAIN
- Catalog number:
- CSL-BLUE
- Category:
- -
- Supplier:
- Cleaver
- Gene target:
- BLUE PROTEIN STAIN
Related genes to: BLUE PROTEIN STAIN
- Gene:
- TYRP1 NIH gene
- Name:
- tyrosinase related protein 1
- Previous symbol:
- TYRP, CAS2
- Synonyms:
- GP75, CATB, TRP, b-PROTEIN, OCA3
- Chromosome:
- 9p23
- Locus Type:
- gene with protein product
- Date approved:
- 1991-09-04
- Date modifiied:
- 2016-04-19
Related products to: BLUE PROTEIN STAIN
Related articles to: BLUE PROTEIN STAIN
- Junken meat sheep display distinct coat-color phenotypes, including a white body with a black head and an entirely black coat at birth, but the transcriptional basis of variation across skin regions and phenotypic backgrounds remains unclear. We performed bulk RNA sequencing (RNA-seq) on nine skin samples from six 1-month-old lambs: black-haired head skin (BT), white-haired dorsal trunk skin (WT), and black-haired dorsal trunk skin from lambs born entirely black (B), with three biological replicates per group. The paired BT-WT comparison characterized within-individual regional differences, whereas B-WT represented a composite phenotypic-background comparison. Cross-comparison integration identified 2056 region-associated genes, 8027 phenotypic-background-associated genes, and 5615 shared genes. Region-associated genes were enriched in mitochondrial and lipid-metabolic processes, phenotypic-background-associated genes in cell communication and adhesion, and shared genes in epidermal keratinization, cytoskeletal organization, and extracellular matrix-integrin-related processes. Network analysis identified a pigmentation-effector subnetwork containing , , , , and . Signature scoring suggested that some contractile signals may reflect tissue-composition differences. Reverse transcription quantitative PCR broadly supported the RNA-seq expression trends. These findings provide a multilayered transcriptional framework for subsequent functional validation of coat-color variation in Junken meat sheep. - Source: PubMed
Publication date: 2026/08/26
Xi BinpengZhao SanchuanYu QianZhang WenzheChen YanWang ZhipengYang HuaLiu Jianbin - The short lifespan of primary normal choroidal melanocytes (NCMs) in vitro represents a major barrier to mechanistic, functional, and translational studies of choroid biology and uveal melanoma (UM). This study aimed to establish and characterize immortalized human NCM lines that retain melanocytic function, maintain a non-cancerous profile, and are amenable to gene editing. - Source: PubMed
Fuentes-Rodriguez AurélieMitchell AndrewGélinas VincentCoutant KellyDroit ArnaudLandreville Solange - Thiosemicarbazones have been widely used due to their various biological activities and interesting NLO behavior. In this study, the thione and thiol tautomers of [(E)-1-[4-[(2,4-dihydroxyphenyl) diazenyl] phenyl] ethylideneamino] thiourea have been studied at the DFT level of theory using BVP86/6-311G(d,p), coupled with molecular docking against tyrosinase, BRAF, and MEK1. The thione tautomer displayed a smaller HOMO-LUMO gap (1.780 eV), greater electrophilicity (9.52 eV), and enhanced NLO characteristics (static β = 134.525 × 10 esu, dynamic β = 3192.119 × 10 esu), along with enhanced binding affinity towards TYRP1 (-8.1 kcal/mol), and inhibitory activity against BRAF (Kᵢ = 0.214 μM), and MEK1 (Kᵢ = 0.497 μM). Based on these results, it is evident that the thione tautomer is a suitable candidate for development as a dual-inhibitor of the tyrosinase/kinase pathways, which can be used for melanoma treatment and also for optoelectronic applications. This work provides the link between theoretical predictions and experimental drug design and forms the basis for future syntheses of thiosemicarbazone-based drugs. - Source: PubMed
Publication date: 2026/08/25
Benhalima NadiaYahia Cherif FatimaKhelloul NawelSindi Emad RashadMoussa Sana BenAlzahrani Abdullah Yahya AbdullahKhelifaoui AhmedUddin Mohammad BorhanAbdelkrim GuendouziZaki Magdi E A - Guangxi Bama miniature pigs are a highly inbred population developed from Bama Xiang pigs and exhibit a stable "two-end black" skin phenotype, making them a useful model for studying regional skin pigmentation. However, the developmental timing and molecular basis of this color difference remain unclear. In this study, black and white skin samples were collected from the posterior head/neck region at six developmental stages: embryonic days 65, 90, and 105, birth, and postnatal days 30 and 180. Histological examination and strand-specific RNA sequencing showed that developmental stage was the major factor shaping skin gene expression, whereas black-white skin differences varied across stages. Transcriptomic differences were already detectable at embryonic day 65, although visible regional differences were not obvious. Pigmentation-associated genes, including , , , and , showed black-skin-biased expression during embryonic development, especially at embryonic day 105. Histological analysis also revealed progressive skin maturation and qualitative differences in darkly stained structures between black and white skin. These findings suggest that molecular differences between black and white skin emerge during embryonic development and persist after birth, providing resources and candidate genes for studying regional pigmentation in Bama miniature pigs. - Source: PubMed
Publication date: 2026/08/17
Feng LingliXia QinXu WenwenDeng ZhongrongAbla AlidaLi JiafuLiang JinningZhang YanpingGuo XiaopingLiang Jing - Abnormal skin pigmentation is a common clinical dermatological issue, and its occurrence and development are closely associated with the inflammatory microenvironment. However, the interactive regulatory mechanisms between inflammatory factors and melanogenesis remain incompletely understood. In this study, through the analysis of transcriptomic data from melasma, psoriasis, acne, atopic dermatitis, and ultraviolet-irradiated skin tissues, we found that CXCL1 may negatively regulate melanogenesis. To validate this finding, we exogenously treated human primary melanocytes, MNT1 cells, and ex vivo human foreskin tissues with CXCL1. The results showed that CXCL1 reduced melanin content, tyrosinase activity, and the expression of key melanogenesis-related genes, including MITF, TYR, TYRP1, and DCT. Mechanistic studies revealed that CXCL1 exerts these inhibitory effects through its canonical receptor CXCR2. Notably, CXCL1 treatment significantly decreased both the expression level and nuclear translocation of β-catenin, a key effector molecule of the WNT signaling pathway, and this effect was effectively reversed by the specific CXCR2 inhibitor SB225002, suggesting that the CXCL1-CXCR2 axis negatively regulates melanogenesis by suppressing the WNT/β-catenin signaling pathway. In summary, this study elucidates a preliminary mechanism by which the CXCL1-CXCR2 axis negatively regulates melanogenesis through inhibition of the WNT/β-catenin signaling pathway, linking inflammatory cytokine networks to the regulatory machinery of melanogenesis. It provides novel perspectives for deciphering the pathogenesis of pigmentary skin disorders and developing therapeutic strategies with combined anti-inflammatory and depigmenting effects. - Source: PubMed
Publication date: 2026/07/31
Zhang YushanZhang FanYu XiaoyuanZhao XiaojiaoChen JingZhu JianjianKang Liyang