PPBT_BOVIN ALPL ELISA tesk kit
- Known as:
- PPBT_BOVIN ALPL Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen17322
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- PPBT_BOVIN ALPL ELISA tesk kit
Related genes to: PPBT_BOVIN ALPL ELISA tesk kit
- Gene:
- ALPL NIH gene
- Name:
- alkaline phosphatase, biomineralization associated
- Previous symbol:
- HOPS
- Synonyms:
- TNSALP, TNALP, TNAP
- Chromosome:
- 1p36.12
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2018-08-08
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- Critical sized craniomaxillofacial bone defects do not heal naturally and often exhibit chronic inflammatory responses that restrict regeneration. It is increasingly apparent that biomaterials must facilitate dynamic crosstalk between immune cells, such as macrophages, and osteoprogenitors to resolve inflammation and accelerate regeneration. Here, we evaluate interactions between macrophages in a neutral (M0) or pro-inflammatory (M1) state with mesenchymal stem cells (MSCs) in a basal or licensed state within a mineralized collagen scaffold. We reveal that MSC-macrophage crosstalk influences significant changes in osteoprogenitor cell differentiation and immune cell polarization. Notably, crosstalk between MSCs and macrophages drives an early-stage inflammatory response, which enhances the immunomodulatory activity of MSCs via secretion of IL-6, an effect that is heightened for already licensed MSCs. The presence of macrophages in the co-cultures upregulated osteogenic (ALPL, BMP2, COL1A2, and RUNX2) and angiogenic genes (ANGPT1) in basal MSC groups. Further, MSC-macrophage interactions subsequently drive increased M2-like macrophage polarization as early as 7 days of culture, as indicated by surface marker expression. These findings show that biomaterial scaffolds can be leveraged as mediators of MSC-mediated immunomodulation with an emphasis on achieving early-stage pro-inflammatory phenotypes that drive subsequent macrophage polarization and markers of increased regenerative potency. - Source: PubMed
Kolliopoulos VasilikiPolanek MaxwellGamage Hashni E VidanaLing Melisande Wong YanTiffany AleczandriaNelson Erik RSpiller Kara LHarley Brendan A C - Nanoplastics (NPs) accumulate in aquatic environments, cross biological barriers, and may hinder or harm early skeletal development in aquatic organisms. Estrogenic endocrine disruptors such as 17α-ethinylestradiol (EE2) can impair craniofacial ossification in fish. However, the effects of NPs on skeletal development in these organisms are not well characterized. Here, we compared the impact of 50 nm polystyrene NPs and EE2 on craniofacial skeletal development and skeletal formation-related gene expression in zebrafish (Danio rerio) larvae, using commercially available fluorescent polystyrene nanoparticles (nominal diameter: 50 nm, aqueous suspension). Embryos were exposed to NPs (5, 50, or 200 μg/mL) or EE2 (10 or 1000 ng/L) between 3 h post-fertilization (hpf) and 10 days post-fertilization (dpf). Operculum area and craniofacial skeletal length were significantly lower in all NPs- and EE2-exposed groups than in the controls. Even low NPs concentrations reduced early-forming skeletal bones. In contrast, it was mainly higher NPs concentrations that inhibited late-forming bones. Principal component analysis (PCA) and hierarchical clustering revealed overlapping skeletal length reduction patterns in the NPs- and EE2-exposed groups. Low NPs exposure downregulated the osteoblast differentiation-related genes runx2 and sp7 whereas higher NPs concentrations repressed the mineralization-related genes alpl, enpp1, and bglap. Similar gene expression patterns were observed for the EE2-exposed groups. The osteoclast-related gene ctsk was downregulated and, therefore, bone resorption was suppressed in the groups exposed to high concentrations of NPs and the EE2-exposed groups. Relative sensitivity to NPs and EE2 type and dosage differed between estrogen receptor subtypes esr1 and esr2a. The findings suggest that NPs exposure may induce alterations in skeletal developmental similar to those observed following EE2 exposure. They also indicate that early-life NPs exposure in zebrafish could influence their skeletal formation. - Source: PubMed
Publication date: 2026/05/28
Yoo SongeunMoon Hye-NaNamgung JinYeo In-Kyu - Osteogenesis imperfecta (OI) is a hereditary connective tissue disorder primarily caused by pathogenic variants in type I collagen genes, while hypophosphatasia (HPP) is a metabolic bone disease resulting from reduced activity of tissue-nonspecific alkaline phosphatase (ALP) due to variants. We report the case of a male infant initially referred for evaluation of femoral bowing and low serum ALP, suggestive of HPP. Prenatal ultrasonography demonstrated limb shortening, and postnatal imaging confirmed femoral bowing; notably, blue sclerae were absent. Biochemical analysis showed persistently low ALP with mildly elevated urinary phosphoethanolamine. Genetic testing identified a heterozygous variant, c.1559delT (p.Leu520ArgfsTer86), a frequent allele in Japan recognized for its association with an asymptomatic carrier phenotype. Subsequent eruption of the primary tooth revealed dentinogenesis imperfecta. Further genetic evaluation demonstrated a previously reported heterozygous variant, c.1135G>A (p.Gly379Arg). Based on the clinical constellation of perinatal femoral deformity, dentinogenesis imperfecta, and absent blue sclerae, a diagnosis of OI, moderate form (Sillence type 4), -related (OI type 4) was established. Although prenatal femoral bowing is a common feature of both OI and HPP, the absence of characteristic rachitic features led us to conclude that the bone abnormalities were consistent with a diagnosis of OI type 4. This case suggests that the c.1559delT carrier state may not significantly modify the phenotype of OI. - Source: PubMed
Publication date: 2026/04/30
Nomura KentoOda YoichiroYamaguchi ShuheiIto TomoyukiNamai Yoshiyuki - The development of scaffolds with osteogenic and angiogenic properties represents an attractive strategy for regenerating large bone defects. This study describes the fabrication and characterization of bioactive glass-based composite scaffolds for bone regeneration applications. Scaffolds comprising polycaprolactone (PCL) and two different bioactive glasses (BGs), derived from white sand (BG-WS) and yellow sand (BG-YS), at an 80:20 wt% ratio were fabricated with a star-shaped pore geometry. Chemical characterization confirmed the presence of characteristic groups from PCL and BGs. The incorporation of BGs led to increased compressive moduli in the composites compared to PCL scaffolds only. Dissolution products, particularly from PCL/BG-YS, promoted enhanced vascular pattern formation of human umbilical endothelial vein cells (HUVECs) co-cultured indirectly with human mesenchymal stromal cells (hMSCs). Direct culture of hMSCs on PCL/BG-WS and PCL/BG-YS composite scaffolds resulted in greater VEGFA production compared to PCL scaffolds alone. The expression of the early osteogenic marker RUNX2 was upregulated on day 7 in response to both composite scaffolds, while ALPL expression increased only with the PCL/BG-WS scaffolds on day 28. Additionally, late osteogenic gene markers (COL1A1 and OCN) were both upregulated on day 28 in PCL/BG-WS scaffolds. Lastly, the measurement of analytes secreted by hMSCs showed higher secretion levels on day 7 than 28, with PCL scaffolds yielding higher concentrations of IL-10, TNF-α, and CCL2 than composite scaffolds. Overall, both PCL/BG-WS and PCL/BG-YS scaffolds demonstrated osteogenic, angiogenic, and potential immunomodulatory properties. Notably, PCL/BG-WS exhibited the strongest osteogenic responses, which may be attributed to its Ti ion content, highlighting the critical role of BG composition in modulating the biological performance of the composites. The findings of this study indicate that combining naturally-derived PO-free BGs with PCL to fabricate 3D composite scaffolds enhances osteogenic and angiogenic properties of the final constructs, emphasizing their potential in bone regeneration. - Source: PubMed
Publication date: 2026/05/20
Nikody MartynaDalfino SophiaHabibovic PamelaMorejón LizetteDelgado José ATartaglia Gianluca MartinoDolci ClaudiaBalmayor Elizabeth RMoroni Lorenzo - Additive manufacturing enables the fabrication of patient-specific zirconia devices with integrated surface features; however, the biological effects of meso-scale topographies remain insufficiently understood. This in vitro study evaluated the influence of defined meso-scale surface modifications on osteoblast behavior using Digital Light Processing (DLP)-fabricated 3Y tetragonal zirconia polycrystal (3Y-TZP) and 5Y partially stabilized zirconia (5Y-PSZ). Planar control specimens and surfaces incorporating regularly distributed columnar structures (height: 100 µm; width: 40 µm; center-to-center spacing: 80, 120, and 160 µm; Mod-80, Mod-120, Mod-160) were fabricated and characterized after sintering. Cytotoxicity was assessed by elution testing and showed cell viability >98% for all groups. Osteoblast adhesion and proliferation (hFOB 1.19) were quantified using metabolic assays. Meso-scale modifications significantly increased early cell adhesion compared to planar controls ( < 0.05), with the strongest effect observed for Mod-160. No significant differences in proliferation rates were detected between groups ( > 0.05). Osteogenic differentiation was evaluated by RT-qPCR (RUNX2, ALPL, COL1A1, BGLAP), revealing material- and geometry-dependent responses. On 3Y-TZP, meso-scale structures, particularly Mod-160, were associated with sustained upregulation of BGLAP, whereas 5Y-PSZ exhibited less pronounced effects. Within the limitations of this in vitro study, meso-scale surface structuring of additively manufactured zirconia enhances early osteoblast adhesion without affecting proliferation and may influence osteogenic differentiation in a material-dependent manner. - Source: PubMed
Publication date: 2026/04/24
Hetzler SebastianRues StefanZenthöfer AndreasRammelsberg PeterKühle ReinaldLux Christopher JErber RalfRoser Christoph J