BAX_HUMAN BCL2L4 ELISA tesk kit
- Known as:
- BAX_HUMAN BCL2L4 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen17244
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- BAX_HUMAN BCL2L4 ELISA tesk kit
Related genes to: BAX_HUMAN BCL2L4 ELISA tesk kit
- Gene:
- BAX NIH gene
- Name:
- BCL2 associated X, apoptosis regulator
- Previous symbol:
- -
- Synonyms:
- BCL2L4
- Chromosome:
- 19q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 1994-11-08
- Date modifiied:
- 2016-10-05
Related products to: BAX_HUMAN BCL2L4 ELISA tesk kit
Related articles to: BAX_HUMAN BCL2L4 ELISA tesk kit
- This study aimed to synthesize a liposomal nanoformulation containing citrinin (LP-CIT) to improve its availability and to evaluate its toxicogenomic profile, focusing on cytotoxic and genotoxic effects in breast cancer (MDA-MB-231) and non-tumorigenic mammary epithelial (MCF-10A) cell lines. The formulation was characterized by macroscopic appearance, pH, particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency, and estability of nanoparticles. Citrinin content, encapsulation efficiency, and liposome stability were evaluated. Cytotoxicity was assessed using MTT, trypan blue exclusion, and flow cytometry. Cell death mechanisms were investigated by mitochondrial membrane potential analysis and expression of apoptotic markers (caspase-9 and BAX). Genotoxicity was evaluated using the comet assay. The liposomal nanosystem consisted of small unilamellar vesicles with a mean diameter of 143.3 nm, a PDI of 0.3, and a positive surface charge (+ 28.5 mV). The average was 63.69 ± 0.18% of citrinin encapsulated in liposomes with stability more than 21 days. Both free citrinin (CIT) and LP-CIT showed cytotoxic effects in MDA-MB-231 and MCF-10A cells, with lower LC₅₀ values observed for LP-CIT. Although LP-CIT induced more pronounced cytotoxic and genotoxic effects in the cancer cell line, toxic effects were also observed in non-tumorigenic cells, indicating limited selectivity. Cell death was associated with mitochondrial membrane depolarization and increased caspase-9 and BAX expression. Both formulations induced DNA damage as detected by the comet assay. Liposomal encapsulation enhanced citrinin biological activity but did not indicate tumor selectivity. Thus, this study represents a toxicogenomic assessment of citrinin and its liposomal formulation for potential therapeutic applicability. - Source: PubMed
Publication date: 2026/07/21
de Menezes Ag-Anne Pereira Melodo Nascimento Maria Luísa Lima BarretoDos Reis Antonielly CampinhoMoura Michely Laiany VieiraNobre Taline Alvesde Sousa Athanara AlvesMartins Jorddam AlmondesFrancisco Sérgio Eduardo Matos Cazarottido Lago João Pedro Alves Damacenode Sousa Igor Gabriel BarbosaNegreiros Helber AlvesGualter Marjorie PereiraBraz Débora CavalcanteGonçalves Juan Carlos RamosSobral Marianna VieiraMarques Karinne Kelly GadelhaCastro Isione OliveiraRamalho Thais CruzFerreira Paulo Michel PinheiroDittz Daltonda Silva Souza Brunada Silva Felipe Cavalcanti CarneiroRolim Hercilia Maria Linsde Castro E Sousa João Marcelo - Triple-negative breast cancer (TNBC) remains one of the most aggressive breast cancer subtypes with limited targeted therapeutic options, highlighting the need for innovative, biocompatible treatment strategies. Plant-mediated green synthesis offers a sustainable route for gold nanoparticle (AuNP) fabrication with enhanced biological functionality. In this study, Ziziphus nummularia ethanolic extract (ZNE) was employed as a reducing and capping agent to synthesize stable, crystalline, and predominantly spherical gold nanoparticles (ZNE-AuNPs). Comprehensive physicochemical characterization confirmed successful nanoparticle formation and phytochemical capping. Importantly, this work provides novel biological and mechanistic insights into the anticancer effects of ZNE-AuNPs specifically in TNBC. ZNE-AuNPs exhibited notable antioxidant activity and significantly reduced the viability of MDA-MB-231 TNBC cells in a concentration- and time-dependent manner. For the first time, the anticancer activity of ZNE-AuNPs in TNBC was mechanistically linked to the suppression of cellular proliferation (decreased Ki-67), induction of apoptosis (modulation of BAX, BCL-2, and caspase-3), and inhibition of ERK phosphorylation, implicating the MAPK/ERK signaling pathway. Taken together, these findings support Ziziphus nummularia-derived AuNPs as a sustainable nanotherapeutic platform and establish their previously unexplored mechanistic relevance in TNBC. - Source: PubMed
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Publication date: 2026/07/20
Zhi YunyunOu JiayiWu DexinZhou KeliLi YonghuiNiu HaiyanRen Shouzhong - Arsenic trioxide (ATO) is effective against FLT3-mutant acute promyelocytic leukemia (APL), but its role in non-APL FLT3-ITD-mutant acute myeloid leukemia (AML) remains unclear. This study aims to investigate the mechanistic impact of ATO on FLT3-ITD-mutated AML cells. - Source: PubMed
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