SLPI_HUMAN WFDC4 ELISA tesk kit
- Known as:
- SLPI_HUMAN WFDC4 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen16905
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- SLPI_HUMAN WFDC4 ELISA tesk kit
Related genes to: SLPI_HUMAN WFDC4 ELISA tesk kit
- Gene:
- SLPI NIH gene
- Name:
- secretory leukocyte peptidase inhibitor
- Previous symbol:
- -
- Synonyms:
- HUSI-I, ALK1, ALP, BLPI, HUSI, WAP4, WFDC4
- Chromosome:
- 20q13.12
- Locus Type:
- gene with protein product
- Date approved:
- 1998-04-24
- Date modifiied:
- 2014-11-19
Related products to: SLPI_HUMAN WFDC4 ELISA tesk kit
Related articles to: SLPI_HUMAN WFDC4 ELISA tesk kit
- Tomato is an essential horticultural and commercial crop, with increasingly Phytophthora infestans encroached, which seriously restrict the quality and productivity. Prior studies have reported that long non-coding RNAs (lncRNAs) play an important role in planta immune response, however, the function of key lncRNAs in response tomato resistant to P. infestans remain to be explored. Here, we identified Sl-lncRNA39042, a 311-bp sense-stranded lncRNA, as a positive regulator of tomato resistance to late blight, regarding that the observation that overexpression of Sl-lncRNA39042 significantly reduced the relative lesion area, whereas its silencing led to a notable increase. Consistent with this, the expression of SlPRs was potentiated in leaves transiently overexpressing Sl-lncRNA39042 but attenuated in Sl-lncRNA39042-silenced leaves upon P. infestans infection. Subsequently, we further found that the expression patterns of SlPI-like and SlPAL-like, two genes located within 100 kb flanking of Sl-lncRNA39042, closely mirrored that of the lncRNA and showed a significant positive correlation. Moreover, both SlPI-like and SlPAL-like were upregulated in leaves transiently overexpressing Sl-lncRNA39042 and downregulated in Sl-lncRNA39042-silenced leaves, suggesting that Sl-lncRNA39042 modulates their expression. Further, we demonstrated that overexpression of either SlPI-like or SlPAL-like significantly enhanced tomato resistance to late blight. Collectively, these results strongly suggest that P. infestans-inducible Sl-lncRNA39042 confers late blight resistance in tomato by activating the expression of SlPI-like and SlPAL-like. These findings expand understanding of the role of lncRNAs in tomato against P. infestans and provide new insights for disease-resistant breeding. - Source: PubMed
Publication date: 2026/06/02
Lv RuiliYao YaoMa NingLuan Yushi - This study integrated and analyzed two sets of gene expression data related to intervertebral disc degeneration (IVDD) to elucidate its key molecular mechanisms. Through screening and enrichment analysis of differentially expressed genes (DEGs), 112 DEGs were identified, primarily involved in extracellular matrix remodeling, cytoplasmic translation, and signaling pathways such as PI3K-Akt. A protein-protein interaction network combined with LASSO and SVM-RFE machine learning algorithms identified 13 hub genes. Immune infiltration analysis revealed reduced infiltration of suppressor cells and monocytes in IVDD samples. In an IL-1β-induced human nucleus pulposus cell degeneration model, qPCR and Western blot experiments confirmed significant downregulation of ADM, ITGB5, RTN4, SLPI, and CSNK1E expression. This study systematically reveals the potential molecular networks and immune characteristics of IVDD, providing new candidate biomarkers and therapeutic insights for subsequent targeted drug development. - Source: PubMed
Publication date: 2026/04/22
Lo HaojuTsai ChunhaoHuang Tsanwen - Immune dysregulation is increasingly recognized as an important contributor to the pathophysiology of irritable bowel syndrome (IBS). This study aimed to identify immune-related core genes in IBS and predict potential regulatory traditional Chinese medicines (TCMs). Two IBS-related Gene Expression Omnibus datasets were integrated and analyzed to identify differentially expressed genes (DEGs). Functional enrichment, immune cell infiltration profiling, and intersection with ImmPort immune-related genes were performed. Core genes were selected through 3 machine-learning algorithms, and potential TCMs were predicted using the Coremine Medical database. A total of 95 DEGs were identified, including 56 upregulated and 39 downregulated genes. Enrichment analyses indicated involvement in muscle system processes, membrane-associated structures, and peptidase inhibitor activity, with significant enrichment in the neuroactive ligand-receptor interaction pathway. Immune infiltration analysis showed increased M2 macrophages, resting natural killer cells, resting dendritic cells, and activated mast cells in IBS samples. Seven immune-related DEGs were obtained, among which LEFTY1, SLPI, and INSL5 were consistently recognized as core genes across all machine-learning approaches. These genes exhibited distinct immune regulatory relevance. Five TCMs: Drynaria fortunei, Crataegus pinnatifida, Houttuynia cordata, Poria cocos, and Bubalus bubalis horn were predicted as potential therapeutic agents targeting the core genes. LEFTY1, SLPI, and INSL5 represent key immune-related genes in IBS and may contribute to its immune regulatory mechanisms. The predicted TCMs provide potential candidates for further validation in IBS management. - Source: PubMed
Bai WeiQian ZixingYang YangHuang GuodongRao XianjunLi HaoZhou TingtingWei Wei - Titanium and its alloys are used in dental and orthopedic implants. However, long-term stability remains a clinical challenge. To overcome this limitation, surface modification has been investigated to improve surface properties. Our previous study demonstrated that the immobilization of secretory leukocyte protease inhibitor (SLPI) on the titanium surface promotes osteoblast adhesion, proliferation, and differentiation in vitro. The current study demonstrated the first in vivo evaluation of SLPI as a bioactive coating for medical implants. Grade 5 titanium screws were coated with 10 µg/mL of recombinant human SLPI (rhSLPI) for 24 h via simple physical adsorption, and the results were preliminarily validated via FE-SEM and ELISA. These SLPI-coated titanium screws (TiSs) were then placed in the tibia of Sprague-Dawley rats for 4 and 8 weeks. The hematological and biochemical parameters (BUN, Creatinine, AST, and Troponin I) demonstrated no acute systemic alterations within the 8-week period across all groups. Moreover, micro-computed tomography (micro-CT) and histological analysis revealed significantly higher bone volume fraction (%BV/TV) at 4 weeks compared to uncoated controls (20.64% ± 2.452% vs. 11.73% ± 0.524%). Finally, the biomechanical stability of implants, assessed using the removal torque test, showed that TiSs showed higher strength compared to Ti at both 4 and 8 weeks. In conclusion, this study represents a novel approach to transitioning rhSLPI-coated titanium evaluation from in vitro models to an in vivo rat model. rhSLPI surface functionalization enhances early-stage osseointegration and improves implant mechanical stability without acute hematological and biochemical alterations. These proof-of-concept findings suggest the potential of SLPI as a bioactive coating strategy. - Source: PubMed
Publication date: 2026/04/20
Chouyratchakarn WannapatBoonsri BurinTangkamonsri SurasakThepsupa WatcharaSupanchart ChayaropKumphune Sarawut - Esophageal cancer is the seventh leading cause of cancer-related deaths, with most cases diagnosed at locally advanced stages. Chemoradiotherapy is one of the most effective treatments for these patients. However, high rates of recurrence persist, highlighting the need for more reliable prognostic biomarkers. In this study, we identified immune-related prognostic genes through bulk (n = 119) and single-cell (n = 60) transcriptomic analyses. We then used Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence to preliminarily investigate the potential biological functions of the candidate biomarker. The clinical prognostic utility of the candidate biomarker was validated in an independent cohort (n = 91) via ELISA of serum samples collected before and during chemoradiotherapy. The results showed that low expression of the secretory leukocyte peptidase inhibitor (SLPI) significantly correlated with tumor progression and poor prognosis. In epithelial cells, reduced SLPI expression was associated with decreased activation of differentiation-related pathways. Furthermore, SLPI expression levels were found to influence fibroblast phenotypes, with exogenous SLPI inhibiting the NF-κB pathway and restoring fibroblast quiescence. The results of ELISA showed that dynamic changes in serum SLPI levels during chemoradiotherapy serve as an independent prognostic factor. In conclusion, serum SLPI levels represent an easy-to-detect biomarker for dynamically monitoring the efficacy of chemoradiotherapy. - Source: PubMed
Publication date: 2026/04/20
Deng HongmingLi JunyiGao Lin-RuiHuang NingWang ChenxiXiao ZefenWang XiaobingWang LimingXiao TingLiu Mei