PIGR_HUMAN PIGR ELISA tesk kit
- Known as:
- PIGR_HUMAN PIGR Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen16543
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- PIGR_HUMAN PIGR ELISA tesk kit
Related genes to: PIGR_HUMAN PIGR ELISA tesk kit
- Gene:
- PIGR NIH gene
- Name:
- polymeric immunoglobulin receptor
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 1989-06-06
- Date modifiied:
- 2016-10-05
Related products to: PIGR_HUMAN PIGR ELISA tesk kit
Related articles to: PIGR_HUMAN PIGR ELISA tesk kit
- The neutralizing activity present in human serum is considered a correlate of protection against SARS-CoV-2 infection and disease but the mechanisms by which serum antibodies are transported into the lumen of the respiratory tract, where they are required to interact with virus particles and infected cells remain incompletely understood. The transcytosis and neutralizing activity of serum-derived IgG and IgA antibodies was investigated using an in vitro SARS-CoV-2 infection model with primary differentiated human nasal and basal epithelial cells (hNECs and hBECs) cultures. Expression of the antibody transport receptors neonatal Fc receptor (FcRn) and polymeric immunoglobulin receptor (pIgR) in hNECs cultures was confirmed by qPCR, immunofluorescence microscopy, and flow cytometry. Both receptors were expressed throughout the epithelial cultures, with enriched expression observed in ciliated cells compared with goblet and basal cells. Purified IgG and IgA isolated from convalescent plasma demonstrated specificity for SARS-CoV-2 spike protein and inhibited ACE2-Spike interactions, although activity was reduced against later variants. Purified IgG contained higher anti-spike antibody titers than purified IgA. Functional neutralization assays showed that transcytosed IgG and IgA significantly reduced SARS-CoV-2 infection compared with untreated controls. However, serial dilution studies demonstrated that IgG-mediated neutralization was more potent than IgA-mediated neutralization. Similar results were determined for influenza A virus H3N2 subtype. The transcytosis of IgG was more efficient in hBEC cultures while IgA transcytosis was higher in hNEC cultures, reflecting the levels of the corresponding transport proteins. Together, these findings demonstrate that serum-derived IgG and IgA can undergo transepithelial transport across human nasal epithelium while retaining SARS-CoV-2 or influenza A virus neutralizing activity in vitro. These results suggest that FcRn- and pIgR-mediated antibody transport may contribute to mucosal protection following vaccination or infection and may help identify antibody responses associated with protection against SARS-CoV-2. - Source: PubMed
Publication date: 2026/05/27
Anaya Eduardo UVue YeeLi MaggieResnick Jessica DSwanson Nico JSullivan David JPekosz Andrew - Porokeratosis (PK) encompasses genetically heterogeneous keratinization disorders, with disseminated superficial actinic porokeratosis (DSAP) and porokeratosis ptychotropica (PPt) as distinct subtypes. Although MVK is a known causative gene, how different variants drive distinct phenotypes remains unclear. Through whole-exome sequencing of two DSAP patients, we identified an MVK variant (c.439G > A, p.Ala147Thr) cataloged as likely pathogenic in ClinVar yet uncharacterized functionally in DSAP keratinocytes. A previously reported PPt-associated variant (c.64G > A) was included for comparison. We established HaCaT cells stably overexpressing each mutant via lentiviral transduction and validated expression by qPCR and Western blot. Integrated transcriptomic and proteomic analyses identified differentially expressed genes (DEGs) and proteins (DEPs) across MVK439 versus control, MVK64 versus control, and MVK439 versus MVK64 groups, followed by GO and KEGG enrichment. Transcriptomic profiling revealed 231, 1,849, and 2,329 DEGs in the respective comparisons. Proteomic screening identified 2,673 DEPs, with 42 shared across all groups, 77 specifically associated with c.439G > A, and 832 linked to c.64G > A. Integrated analysis suggested IL12A and pIgR as potential contributors to c.439G > A-driven DSAP, while CXCL11, CXCL9, and TNFRSF12A may mediate c.64G > A-induced PPt. These findings offer new insights into MVK function and PK pathogenesis, warranting validation in larger cohorts. - Source: PubMed
Publication date: 2026/06/03
Lai ShuqinZhu WenjieLin ChunliGuo ZimengChen ShiqiXie LangLiu JieZeng ZhaolinYou CongLi Longnian - This research systematically compared the jejunum morphology, antioxidant capacity, barrier-related gene expression, and mucosal immune status between two Yunnan local pig breeds, namely Dahe pigs (DH) and Dahe black pigs (DHB), using histopathological staining, antioxidant assays, quantitative polymerase chain reaction (qPCR), immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). The outcomes indicated that DHB presented a shallower crypt depth and a higher ratio of villus height to crypt depth, suggesting an enhanced capacity for nutrient absorption. In their jejunum, the activities of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were significantly elevated, whereas the levels of malondialdehyde (MDA) were notably reduced. Conversely, DH displayed superior barrier function, with significantly higher expression levels of tight-junction proteins (Occludin, Claudin-1, and Claudin-2, , mucin genes (), defense-related proteins (), and immune-related genes (Interleukin-10, Toll-like receptor 4, Myeloid differentiation factor 88, Immunoglobulin heavy chain A, Joining chain, and Polymeric immunoglobulin receptor, ), along with a significant increase in IgG levels ( < 0.05). These findings uncover the distinct physiological and immunological characteristics between the two breeds, offering theoretical underpinnings for precision nutrition and healthy breeding strategies in local pig populations. - Source: PubMed
Publication date: 2026/05/13
Jia HuijinShi WenzheXue ShiqiZhang WanghongSong GuangyaoYi LanlanZhu JunhongZhao Sumei - is a significant intracellular pathogen of channel catfish () and a major threat to U.S. aquaculture. A recently developed recombinant attenuated vaccine strain (χ16016) uses arabinose-regulated expression to trigger delayed cell wall lysis in vivo, ensuring biological containment while conferring strong protection against virulent challenge. Although its efficacy has been demonstrated, the host immune programs underlying protection remain incompletely defined. We used RNA sequencing to characterize tissue-specific transcriptomic responses in the intestines and kidneys of channel catfish at 7 days post-vaccination. Fish were vaccinated with χ16016 by either bath immersion or intracoelomic (IC) injection, and differentially expressed genes and enriched immune pathways were analyzed to determine how the vaccine delivery route shapes systemic and mucosal immune responses. Across comparisons, 19,101 differentially expressed genes revealed pronounced route- and tissue-dependent immune remodeling. As aquaculture vaccination strategies increasingly prioritize scalability and practical deployment, understanding how the delivery route shapes immune outcomes is critical. Here, IC vaccination induced broader systemic transcriptional changes, particularly in the intestine, whereas bath immunization elicited a more focused yet coordinated mucosal response. Overall, intestinal tissue exhibited greater transcriptional responsiveness than kidney tissue, underscoring its central role in early vaccine-induced immunity. Functional enrichment analyses identified the activation of innate recognition pathways, MAPK and calcium signaling cascades, complement components, antigen processing machinery, and cell adhesion networks. Notably, bath immunization enriched the pathway, which represents an orthology-based mapping of conserved mucosal immune components, alongside the upregulation of IL-6, CXCL12-CXCR4, integrins (α4β7), MHC class II, complement C3, and polymeric immunoglobulin receptor (pIgR). Given that catfish rely primarily on IgM in mucosal immunity, these findings indicate the induction of IgM-mediated mucosal defense rather than classical mammalian IgA responses. Concurrent complement and scavenger receptor signatures suggest a transition toward efficient opsonophagocytic clearance with controlled inflammation at this subacute stage. This study provides the first systems-level view of host transcriptomic responses to a regulated-lysis vaccine in channel catfish. The findings demonstrate that immersion vaccination, although transcriptionally less expansive than injection, effectively activates coordinated mucosal innate and adaptive immune programs, supporting its practical use as a scalable vaccination strategy for aquaculture. - Source: PubMed
Publication date: 2026/05/01
Miryala Kavi RCurtiss RoyLima ViniciusSwain Banikalyan - Shiga toxin-producing Escherichia coli O157:H7 (O157), a foodborne human pathogen, persists at the rectoanal junction (RAJ) of the bovine intestinal tract, in asymptomatic cattle reservoirs. Identifying mechanisms used by O157 for initial adherence before persistence at the RAJ could help develop effective O157 control modalities. We recently established the role of carbon starvation-inducible lipoprotein (Slp) in initial adherence of O157 to Caco-2 cells, with the human polymeric immunoglobulin receptor (pIgR) protein as the Slp-receptor. Here, we evaluated the role of Slp in O157 adherence to the bovine RAJ using the RAJ squamous epithelial (RSE) cell- and RAJ-in vitro Organ Culture (IVOC)- adherence assays. The wild-type O157 strain EDL932 (EDL932-WT), it's isogenic slp deletion mutant (EDL932 Δslp), and the slp complemented mutant (EDL932 Δslp-p:slp), were tested with no bacteria controls. Adherence was verified by culture and immunofluorescence (IF) staining of O157. Tissue integrity was determined using nuclear/cell staining dyes and histopathological examination. All test strains adhered in a diffuse-moderate pattern on RSE cells. However, differential adherence was observed on the RAJ-IVOC with the strains preferentially adhering to the columnar cells. Additionally, EDL932-WT and EDL932 Δslp-p:slp strains adhered in slightly greater numbers than the EDL932 Δslp strain to the RAJ-IVOC, causing disruptions primarily in the columnar region of otherwise intact RAJ-IVOC tissues. Interestingly, pIgR was also predominantly detected by IF microscopy and RNAscope in situ hybridization at the columnar region of the RAJ-IVOC tissue. In silico modeling demonstrated the possibility of a bovine pIgR- bacterial Slp interaction. Hence, our observations support the role for Slp in the initial adherence of O157 to the columnar cells at the bovine RAJ, unlike the squamous cells where the loss of slp did not impact attachment. In addition, a possible mucosal immune-interference resulting from the bovine pIgR-Slp interaction may contribute towards long-term O157 colonization of cattle. - Source: PubMed
Publication date: 2026/05/18
Kudva Indira TBiernbaum Erika NCassmann Eric DPalmer Mitchell VEdison Lekshmi KCastellanos-Gell JessyKariyawasam Subhashinie