PIGR_HUMAN PIgR ELISA tesk kit
- Known as:
- PIGR_HUMAN PIgR Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen16542
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- PIGR_HUMAN PIgR ELISA tesk kit
Related genes to: PIGR_HUMAN PIgR ELISA tesk kit
- Gene:
- PIGR NIH gene
- Name:
- polymeric immunoglobulin receptor
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 1989-06-06
- Date modifiied:
- 2016-10-05
Related products to: PIGR_HUMAN PIgR ELISA tesk kit
Related articles to: PIGR_HUMAN PIgR ELISA tesk kit
- This research systematically compared the jejunum morphology, antioxidant capacity, barrier-related gene expression, and mucosal immune status between two Yunnan local pig breeds, namely Dahe pigs (DH) and Dahe black pigs (DHB), using histopathological staining, antioxidant assays, quantitative polymerase chain reaction (qPCR), immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). The outcomes indicated that DHB presented a shallower crypt depth and a higher ratio of villus height to crypt depth, suggesting an enhanced capacity for nutrient absorption. In their jejunum, the activities of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were significantly elevated, whereas the levels of malondialdehyde (MDA) were notably reduced. Conversely, DH displayed superior barrier function, with significantly higher expression levels of tight-junction proteins (Occludin, Claudin-1, and Claudin-2, , mucin genes (), defense-related proteins (), and immune-related genes (Interleukin-10, Toll-like receptor 4, Myeloid differentiation factor 88, Immunoglobulin heavy chain A, Joining chain, and Polymeric immunoglobulin receptor, ), along with a significant increase in IgG levels ( < 0.05). These findings uncover the distinct physiological and immunological characteristics between the two breeds, offering theoretical underpinnings for precision nutrition and healthy breeding strategies in local pig populations. - Source: PubMed
Publication date: 2026/05/13
Jia HuijinShi WenzheXue ShiqiZhang WanghongSong GuangyaoYi LanlanZhu JunhongZhao Sumei - is a significant intracellular pathogen of channel catfish () and a major threat to U.S. aquaculture. A recently developed recombinant attenuated vaccine strain (χ16016) uses arabinose-regulated expression to trigger delayed cell wall lysis in vivo, ensuring biological containment while conferring strong protection against virulent challenge. Although its efficacy has been demonstrated, the host immune programs underlying protection remain incompletely defined. We used RNA sequencing to characterize tissue-specific transcriptomic responses in the intestines and kidneys of channel catfish at 7 days post-vaccination. Fish were vaccinated with χ16016 by either bath immersion or intracoelomic (IC) injection, and differentially expressed genes and enriched immune pathways were analyzed to determine how the vaccine delivery route shapes systemic and mucosal immune responses. Across comparisons, 19,101 differentially expressed genes revealed pronounced route- and tissue-dependent immune remodeling. As aquaculture vaccination strategies increasingly prioritize scalability and practical deployment, understanding how the delivery route shapes immune outcomes is critical. Here, IC vaccination induced broader systemic transcriptional changes, particularly in the intestine, whereas bath immunization elicited a more focused yet coordinated mucosal response. Overall, intestinal tissue exhibited greater transcriptional responsiveness than kidney tissue, underscoring its central role in early vaccine-induced immunity. Functional enrichment analyses identified the activation of innate recognition pathways, MAPK and calcium signaling cascades, complement components, antigen processing machinery, and cell adhesion networks. Notably, bath immunization enriched the pathway, which represents an orthology-based mapping of conserved mucosal immune components, alongside the upregulation of IL-6, CXCL12-CXCR4, integrins (α4β7), MHC class II, complement C3, and polymeric immunoglobulin receptor (pIgR). Given that catfish rely primarily on IgM in mucosal immunity, these findings indicate the induction of IgM-mediated mucosal defense rather than classical mammalian IgA responses. Concurrent complement and scavenger receptor signatures suggest a transition toward efficient opsonophagocytic clearance with controlled inflammation at this subacute stage. This study provides the first systems-level view of host transcriptomic responses to a regulated-lysis vaccine in channel catfish. The findings demonstrate that immersion vaccination, although transcriptionally less expansive than injection, effectively activates coordinated mucosal innate and adaptive immune programs, supporting its practical use as a scalable vaccination strategy for aquaculture. - Source: PubMed
Publication date: 2026/05/01
Miryala Kavi RCurtiss RoyLima ViniciusSwain Banikalyan - Shiga toxin-producing Escherichia coli O157:H7 (O157), a foodborne human pathogen, persists at the rectoanal junction (RAJ) of the bovine intestinal tract, in asymptomatic cattle reservoirs. Identifying mechanisms used by O157 for initial adherence before persistence at the RAJ could help develop effective O157 control modalities. We recently established the role of carbon starvation-inducible lipoprotein (Slp) in initial adherence of O157 to Caco-2 cells, with the human polymeric immunoglobulin receptor (pIgR) protein as the Slp-receptor. Here, we evaluated the role of Slp in O157 adherence to the bovine RAJ using the RAJ squamous epithelial (RSE) cell- and RAJ-in vitro Organ Culture (IVOC)- adherence assays. The wild-type O157 strain EDL932 (EDL932-WT), it's isogenic slp deletion mutant (EDL932 Δslp), and the slp complemented mutant (EDL932 Δslp-p:slp), were tested with no bacteria controls. Adherence was verified by culture and immunofluorescence (IF) staining of O157. Tissue integrity was determined using nuclear/cell staining dyes and histopathological examination. All test strains adhered in a diffuse-moderate pattern on RSE cells. However, differential adherence was observed on the RAJ-IVOC with the strains preferentially adhering to the columnar cells. Additionally, EDL932-WT and EDL932 Δslp-p:slp strains adhered in slightly greater numbers than the EDL932 Δslp strain to the RAJ-IVOC, causing disruptions primarily in the columnar region of otherwise intact RAJ-IVOC tissues. Interestingly, pIgR was also predominantly detected by IF microscopy and RNAscope in situ hybridization at the columnar region of the RAJ-IVOC tissue. In silico modeling demonstrated the possibility of a bovine pIgR- bacterial Slp interaction. Hence, our observations support the role for Slp in the initial adherence of O157 to the columnar cells at the bovine RAJ, unlike the squamous cells where the loss of slp did not impact attachment. In addition, a possible mucosal immune-interference resulting from the bovine pIgR-Slp interaction may contribute towards long-term O157 colonization of cattle. - Source: PubMed
Publication date: 2026/05/18
Kudva Indira TBiernbaum Erika NCassmann Eric DPalmer Mitchell VEdison Lekshmi KCastellanos-Gell JessyKariyawasam Subhashinie - Polymeric immunoglobulin receptor (PIGR) is a transmembrane protein widely expressed in mucosal epithelial cells that is involved in the transcytosis of the polymeric immunoglobulins IgA and IgM. Recent findings revealed increased plasma PIGR levels in subjects with subclinical atherosclerosis, although its function remains uncertain. - Source: PubMed
Publication date: 2026/04/23
Cerro-Pardo IsabelPicatoste BelénRaposo-Gutiérrez IreneMartos-Folgado InmaculadaMárquez-Gálvez CristinaGarcía-García AnaOrtega-Villanueva LucíaMur Sonia MRobles-Vera IñakiEscolà-Gil Joan CarlesNúñez EstefaníaSancho DavidLindholt JesVázquez JesúsBlanco-Colio Luis MiguelRamiro Almudena RMartín-Ventura José Luis - Mass spectrometry (MS)-based proteomics approaches have proven to be a powerful tool to discover clinical biomarkers for dental caries diagnosis and management. Saliva, acquired enamel pellicle (AEP), and dentin are three common sample medium. However, MS-based proteomics of dental caries is currently facing two major problems: (1) the analytical coverage of the dental caries proteome is relatively limited (<3000 proteins), which may hamper our understanding of the function of the key but low-abundance proteins. (2) gingival crevicular fluid (GCF) is a valuable oral fluid, but the association of GCF proteins and dental caries remains underexplored and the GCF proteomic profile in the context of dental caries has not yet been characterized. - Source: PubMed
Publication date: 2026/04/16
Yang XueZhao TingYu PingboCai DanLiao RijingCai YanWang Jun