Active Human Calpain I100 ug
- Known as:
- Active Human Calpain I100 ug
- Catalog number:
- 1134-100
- Product Quantity:
- 100 ug
- Category:
- -
- Supplier:
- Biovis
- Gene target:
- Active Human Calpain I100
Related genes to: Active Human Calpain I100 ug
- Gene:
- TAF5 NIH gene
- Name:
- TATA-box binding protein associated factor 5
- Previous symbol:
- TAF2D
- Synonyms:
- TAFII100
- Chromosome:
- 10q24.33
- Locus Type:
- gene with protein product
- Date approved:
- 1995-07-07
- Date modifiied:
- 2016-10-05
Related products to: Active Human Calpain I100 ug
Related articles to: Active Human Calpain I100 ug
- To construct a diagnostic model of osteoarthritis related to methylation genes using machine learning algorithms, and analyze its prognostic value and biological functions. - Source: PubMed
Publication date: 2025/08/01
Cui XuJi HoulinGuo ShengyangLiu JuZhang LinyuanJia YongweiCui YinZhou Xiaoxiao - Spermatogenesis is a crucial indicator of geese reproduction performance and production. The testis is the main organ responsible for sperm production, and the egg-laying cycle in geese is a complex physiological process that demands precise orchestration of hormonal cues and cellular events within the testes, however, the seasonal changes in the transcriptomic and proteomic profiles of goose testicles remain unclear. To explore various aspects of the mechanisms of the seasonal cyclicity of testicles in different goose breeds, in this study, we used an integrative transcriptomic and proteomic approach to screen the key genes and proteins in the testes of 2 goose males, the Hungarian white goose and the Wanxi white goose, at 3 different periods of the laying cycle: beginning of laying cycle (BLC), peak of laying cycle (PLC), and end of laying cycle (ELC). The results showed that a total of 9,273 differentially expressed genes and 4,543 differentially expressed proteins were identified in the geese testicles among the comparison groups. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that the DEGs, in the comparison groups, were mainly enrichment in metabolic pathways, neuroactive ligand-receptor interaction, cyctokine-cyctokine receptor interaction, calcium signaling pathway, apelin signaling pathway, ether lipid metabolism, cysteine, and methionine metabolism. While the DEPs, in the 3 comparison groups, were mainly involved in the ribosome, metabolic pathways, carbon metabolism, proteasome, endocytosis, lysosome, regulation of actin cytoskeleton, oxidative phosphorylation, nucleocytoplasmic transport, and tight junction. The protein-protein interaction network analysis (PPI) indicated that selected DEPs, such as CHD1L, RAB18, FANCM, TAF5, TSC1/2, PHLDB2, DNAJA2, NCOA5, DEPTOR, TJP1, and RAPGEF2, were highly associated with male reproductive regulation. Further, the expression trends of 4 identified DEGs were validated by qRT-PCR. In conclusion, this work offers a new perspective on comprehending the molecular mechanisms and pathways involved in the seasonal cyclicity of testicles in the Hungarian white goose and the Wanxi white goose, as well as contributing to improving goose reproductive performance. - Source: PubMed
Publication date: 2024/08/14
Mabrouk IchrafSong YupuLiu QiuyuanMa JingyunZhou YuxuanYu JinHou JiahuiHu XiangmanLi XinyueXue GuizhenCao HengMa XiaomingXu JingWang JingboPan HongxiaoHua GuoqingHu JingtaoSun Yongfeng - CRISPR-Cas technology has transformed functional genomics, yet understanding of how individual exons differentially shape cellular phenotypes remains limited. Here, we optimized and conducted massively parallel exon deletion and splice-site mutation screens in human cell lines to identify exons that regulate cellular fitness. Fitness-promoting exons are prevalent in essential and highly expressed genes and commonly overlap with protein domains and interaction interfaces. Conversely, fitness-suppressing exons are enriched in nonessential genes, exhibiting lower inclusion levels, and overlap with intrinsically disordered regions and disease-associated mutations. In-depth mechanistic investigation of the screen-hit TAF5 alternative exon-8 revealed that its inclusion is required for assembly of the TFIID general transcription initiation complex, thereby regulating global gene expression output. Collectively, our orthogonal exon perturbation screens established a comprehensive repository of phenotypically important exons and uncovered regulatory mechanisms governing cellular fitness and gene expression. - Source: PubMed
Publication date: 2024/06/24
Xiao Mei-ShengDamodaran Arun PrasathKumari BandanaDickson EthanXing KunOn Tyler AParab NikhilKing Helen EPerez Alexendar RGuiblet Wilfried MDuncan GerardChe AnneyChari RajAndresson ThorkellVidigal Joana AWeatheritt Robert JAregger MichaelGonatopoulos-Pournatzis Thomas - The plant corepressor TPL is recruited to diverse chromatin contexts, yet its mechanism of repression remains unclear. Previously, we have leveraged the fact that TPL retains its function in a synthetic transcriptional circuit in the yeast model to localize repressive function to two distinct domains. Here, we employed two unbiased whole genome approaches to map the physical and genetic interactions of TPL at a repressed locus. We identified SPT4, SPT5 and SPT6 as necessary for repression with the SPT4 subunit acting as a bridge connecting TPL to SPT5 and SPT6. We also discovered the association of multiple additional constituents of the transcriptional preinitiation complex at TPL-repressed promoters, specifically those involved in early transcription initiation events. These findings were validated in yeast and plants through multiple assays, including a novel method to analyze conditional loss of function of essential genes in plants. Our findings support a model where TPL nucleates preassembly of the transcription activation machinery to facilitate rapid onset of transcription once repression is relieved. - Source: PubMed
Publication date: 2024/04/01
Leydon Alexander RDowning BenjaminSanchez Janet SolanoLoll-Krippleber RaphaelBelliveau Nathan MRodriguez-Mias Ricard ABauer AndrewWatson Isabella JBae LenaVillén JuditBrown Grant WNemhauser Jennifer L - Early embryonic development is a finely orchestrated process that requires precise regulation of gene expression coordinated with morphogenetic events. TATA-box binding protein-associated factors (TAFs), integral components of transcription initiation coactivators like TFIID and SAGA, play a crucial role in this intricate process. Here we show that disruptions in TAF5, TAF12 and TAF13 individually lead to embryonic lethality in the mouse, resulting in overlapping yet distinct phenotypes. Taf5 and Taf12 mutant embryos exhibited a failure to implant post-blastocyst formation, and Taf5 mutants have aberrant lineage specification within the inner cell mass. In contrast, Taf13 mutant embryos successfully implant and form egg-cylinder stages but fail to initiate gastrulation. Strikingly, we observed a depletion of pluripotency factors in TAF13-deficient embryos, including OCT4, NANOG and SOX2, highlighting an indispensable role of TAF13 in maintaining pluripotency. Transcriptomic analysis revealed distinct gene targets affected by the loss of TAF5, TAF12 and TAF13. Thus, we propose that TAF5, TAF12 and TAF13 convey locus specificity to the TFIID complex throughout the mouse genome. - Source: PubMed
Publication date: 2024/04/07
He Xinjian DorisPhillips ShelbyHioki KaitoMajhi Prabin DhangadaBabbitt CourtneyTremblay Kimberly DPobezinsky Leonid AMager Jesse