ACON_PIG ACO2 ELISA tesk kit
- Known as:
- ACON_PIG ACO2 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen16257
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- ACON_PIG ACO2 ELISA tesk kit
Related genes to: ACON_PIG ACO2 ELISA tesk kit
- Gene:
- ACO2 NIH gene
- Name:
- aconitase 2
- Previous symbol:
- -
- Synonyms:
- ACONM
- Chromosome:
- 22q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2015-12-01
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- Drought is a critical climatic constraint to coffee, but its interaction with enhanced atmospheric [CO] is highly relevant to the crop's sustainability under climate-change scenarios. We explored such interaction through the physiological and biochemical responses of 7-year-old potted plants of two genotypes: cv. Conilon Clone 153 (CL153) and cv. Icatu, grown under well-watered (WW) conditions at ambient [CO] (aCO, 380 µL L) or elevated [CO] (eCO, 700 µL L). Plants were gradually exposed to moderate (MWD) and severe (SWD) water deficits, reaching predawn water potentials (Ψ) between -1.6 and -2.2 MPa (MWD) or ≤ -3.7 MPa (SWD). Under aCO, both genotypes showed full resilience to MWD. Moreover, Icatu exhibited tolerance to SWD regardless of [CO], showing stable chronic photoinhibition (PI), chlorophyll (Chl) content, and moderate osmotic adjustment from MWD to SWD (mostly related to mannitol buildup). It also showed a broader coordinated response involving enhanced photoprotection (e.g., xanthophylls), the reconfiguration of photosystems (Chl (/) decline), and the lipid profile of chloroplast membranes (fatty-acid composition and degree of unsaturation). The latter involved both quantitative ( synthesis) and qualitative changes (unsaturation shift, mostly due to an increase in C18:3 and a decline in C16:0), along with an increased C16:1. Notably, eCO attenuated the declines in Ψ and cell turgor under MWD particularly in Icatu, thus preserving growth. As for CL153, several adverse impacts were found in SWD under aCO (PI, Chl loss, and membrane leakage), which were counteracted by eCO, highlighting its key role in the acquired resilience of this genotype. Collectively, these findings identify reliable traits underpinning the drought resilience of climate-resilient coffee cultivars. - Source: PubMed
Publication date: 2026/07/30
Semedo José NPais Isabel PLeitão António ERodrigues Ana PMarques IsabelSilva Maria JPartelli Fábio LLidon Fernando CDaMatta Fábio MRibeiro-Barros Ana IRamalho José C - Most spp. trees in China are cultivated in acidic soils with low soil organic matter and high Al. The mechanisms of humic acid (HA) to alleviate Al stress in plants remain unclear. 'Sour pummelo' ( (L.) Osbeck) seedlings were exposed to 0.5 (HA0.5), 0.1 (HA0.1), or 0 (HA0) mM sodium humate and 1.2 (Al1.2) or 0 (Al0) mM AlCl·6HO for 128 days. Thereafter, the research examined biomass; Al and mineral nutrients; leaf photosynthetic performance; and leaf and root nonstructural carbohydrates, reactive oxygen species metabolism, and related physiological parameters. Al1.2 significantly reduced whole plant dry weight (DW), root DW, leaf CO assimilation (A), and chlorophyll concentration by 61%, 45%, 61%, and 35%, respectively, at HA0, but only 48%, 17%, 44%, and 11%, respectively, at HA0.5. Further analysis suggested that the addition of HA endowed with Al resilience by the following several aspects: () lessened tissue (leaf, stem, and root) concentrations of Al and enhanced capacity to maintain macronutrient (S, K, Mg, Ca, N, and P) homeostasis at Al1.2; () improved capacity to combat oxidative stress at Al1.2; and () enhanced A and growth at Al1.2. Further analysis indicated that HA-mediated alleviation of growth decline caused by Al1.2 involved () reduced ability to absorb Al and less root-to-shoot Al transport and () increased ability to maintain macronutrient homeostasis and to combat oxidative stress; and that HA-mediated alleviation of leaf chlorophyll and A decline and photosynthetic electron transport chain impairment involved less leaf Al concentration and improved leaf macronutrient homeostasis. To conclude, the addition of HA lowered roots' ability to absorb Al and tissue Al concentration and subsequently mitigated Al-toxic impairment to root growth and function, thereby enhancing the ability of plants to maintain macronutrient homeostasis, and hence alleviating Al1.2-stimulated oxidative damage and inhibition of A and growth. - Source: PubMed
Publication date: 2026/07/31
Shen QianXia Tian-TianTong Liang-YuanLan Bin-BinHuang Wei-LinWu TiYe XinLai Ning-WeiChen Li-Song - Salinity fluctuations pose critical physiological challenges to the sea cucumber Apostichopus japonicus. This review elucidated its molecular salinity response mechanisms, focusing on miRNA-mediated regulation and core pathways. We highlight a hierarchical network in which 8 key miRNAs fine-tune responses. let-7 targeted Solute Carrier Family 34 Member 2 (SLC34A2) and Cystathionine Gamma-Lyase (CTH), coordinating ion transport and amino acid metabolism, respectively. miR-10 targeted genes Nicotinamide Phosphoribosyltransferase (NAMPT) in energy homeostasis and TGF-Beta Activated Kinase 1 (MAP3K7) Binding Protein 1 (Table 1) to regulate MAPK/ERK signaling. miR-278-3p targeted 5-Hydroxytryptamine Receptor 2B (Htr2b) and Chloride Channel Accessory 1 (CLCA1) to modulate GPCR signaling and chloride transport. miR-2008, miR-3, miR-16, miR-22, and miR-14 coordinate vesicular trafficking, transcription, apoptosis, and autophagy via targeting Pleckstrin Homology Domain Containing A3 (PLEKHA3), Upstream Binding Transcription Factor (UBTF), ATP Binding Cassette Subfamily C Member 2 (ABCC2), Polypeptide N-Acetylgalactosaminyltransferase 2 (GALNT2), Glutamic-Oxaloacetic Transaminase 2 (Got2), Aconitase 2 (Aco2), and RNA Polymerase II Associated Protein 2 (Rpap2). Salinity sensing initiates GPCR-mediated cAMP/PKA and MAPK/ERK cascades, activating transcription factors (UBTF) to drive stress gene expression. Ion transporters (SLC family), ABC transporters, and vesicular transport maintain cellular homeostasis. Cullin Associated and Neddylation Dissociated 1 (CAND1)/Listerin E3 ubiquitin protein ligase 1 (Ltn1) are involved in amino acid/protein metabolism and ubiquitinated proteins to participate in vesicular transport for protein sorting and secreting. These secreted molecules (Glyare/Taurine) act as ligands to bind membrane receptors and trigger GPCR pathways and energy metabolism and redox balance. Aco2/Got2/NAMPT supply ATP for adaptation and apoptosis/autophagy, repair damage and maintain homeostasis, alleviate oxidative damage, supported by innate immunity genes. These findings establish a framework for understanding sea cucumbers salinity adaption, further exploration of additional genes is needed to refine mechanistic details. - Source: PubMed
Publication date: 2026/08/10
Chen JunweiZhao YudiLi NanWang HuiWu XuesongWang BaichangWei XinChang YaqingTian Yi - This study investigated the effects of high boron (B) concentrations on photosynthetic performance in Citrus macrophylla. Six-month-old plants were grown under controlled conditions and irrigated for 30 days with nutrient solutions containing 0.11 mg·L¹ (control), 5 mg·L¹ (B5), and 10 mg·L¹ (B10) B. Excess B reduced stomatal conductance (g) and net CO assimilation (A). However, the stronger decline in A relative to g, together with stable intercellular CO concentration (C), indicated that photosynthetic inhibition was not exclusively driven by stomatal closure. Instead, non-stomatal limitations played a key role. Chlorophyll fluorescence analysis showed progressive declines in F/F and ΦPSII with increasing B accumulation, indicating PSII photoinhibition and impaired electron transport. At moderate B levels (B5), regulated non-photochemical energy dissipation (ΦNPQ) was activated. However, at excessive B accumulation (B10), photoprotective mechanisms became insufficient, resulting in increased lipid peroxidation. Photosynthetic capacity analysis revealed a significant decrease in V under high B, while J and TPU remained unaffected. Total soluble sugars and starch concentrations were also not significantly affected by B supply, indicating that the decline in apparent carboxylation capacity occurred without detectable changes in bulk leaf carbohydrate pools. These results are consistent with a predominant biochemical limitation involving reduced apparent Rubisco carboxylation capacity. Additionally, chlorophyll concentration decreased under excess B, further compromising photosynthetic efficiency. At the gene expression level, processes related to cellular functions aiming to maintain the structural cell integrity and plant defence responses are modified, which may take precedence over the preservation of photosynthetic efficiency. Overall, B toxicity in C. macrophylla induced a shift from stomatal to dominant non-stomatal limitations in photosynthesis, involving Rubisco impairment and PSII photochemistry. These findings provide new insights into the mechanisms underlying B-induced photosynthetic decline and highlight the vulnerability of citrus to B toxicity. - Source: PubMed
Publication date: 2026/08/03
Tasa MariaPenella ConsueloArbona VicentGonzalez-Guzmán MiguelMorard MiguelPérez-Pérez Juan G - Rising atmospheric CO has significant implications for crop productivity and food security. Based on studies in C3 plants, elevated CO (eCO) can shape plant-pathogen interactions, although the outcomes are often variable. The question of how eCO influences immunity and disease development in C4 plants, such as the globally important cereal crop maize (Zea mays L.), has not been systematically examined. We challenged maize plants grown under ambient CO (aCO, 420 ppm) and eCO (550 ppm) with bacterial, viral, fungal, and oomycete pathogens. Plants grown in eCO were more susceptible to sugarcane mosaic virus, suggesting compromised antiviral defenses, less susceptible to Clavibacter nebraskensis, Exserohilum turcicum, and Colletotrichum graminicola, and susceptibility to Puccinia sorghi and Pythium sylvaticum was unchanged. Reduced susceptibility to C. nebraskensis was associated with enhanced basal immune responses. These results establish a foundation for dissecting eCO-responsive defense mechanisms, and they highlight a critical need to understand how eCO will impact plant responses to microbes, pests, and abiotic stresses under future conditions. - Source: PubMed
Publication date: 2026/07/31
Khwanbua EkkachaiQi YunhuiSsengo JohnLiu PengGraham Michelle AWhitham Steven A