PLP2_MOUSE Plp2 ELISA tesk kit
- Known as:
- PLP2_MOUSE Plp2 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen16172
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- PLP2_MOUSE Plp2 ELISA tesk kit
Related genes to: PLP2_MOUSE Plp2 ELISA tesk kit
- Gene:
- PLP2 NIH gene
- Name:
- proteolipid protein 2
- Previous symbol:
- -
- Synonyms:
- A4, A4-LSB, MGC126187
- Chromosome:
- Xp11.23
- Locus Type:
- gene with protein product
- Date approved:
- 1998-05-05
- Date modifiied:
- 2016-06-03
Related products to: PLP2_MOUSE Plp2 ELISA tesk kit
Related articles to: PLP2_MOUSE Plp2 ELISA tesk kit
- Clinically actionable biomarkers that reliably distinguish glioblastoma (GBM) from lower-grade glioma (LGG) across expression platforms remain an unmet need. Existing transcriptomic signatures are frequently confounded by batch effects, platform heterogeneity, and the inability to translate to single-patient clinical workflows. We developed a topology-aware biomarker discovery framework in which analysis-of-variance ranking defines a candidate gene pool, hypergraph co-expression analysis at correlation threshold = 0.75 identifies densely connected hubs within this pool, rough set reduct optimisation selects a minimal sufficient subset of these hubs, and a Random Forest classifier with stratified cross-validation performs the final discrimination. The pipeline was trained exclusively on GSE16011, a single-platform single-institution Affymetrix microarray cohort free from batch-class confound, and validated on two independent RNA-sequencing cohorts (CGGA-325 and CGGA-693). Robustness was further assessed through bootstrap optimism correction, DeLong cross-cohort equivalence testing, leave-one-gene-out analysis, and a sensitivity analysis under WHO CNS5 (2021) class definitions. The pipeline identified a ten-gene biomarker panel (, , , , , , , , , and ), achieving a fivefold cross-validation AUROC of 0.906 ± 0.029 and a held-out AUROC of 0.831. External validation yielded AUROC = 0.838 in CGGA-325 and AUROC = 0.836 in CGGA-693. The biomarker-derived risk score demonstrated independent prognostic value in CGGA-693 (multivariate Cox hazard ratio = 9.195; < 0.001) after adjustment for WHO histological grade, with Kaplan-Meier analysis confirming highly significant survival separation (log-rank = 4.60 × 10). Class definitions in the present work follow the histology-based pre-2021 WHO classification used in the source datasets and do not directly incorporate WHO CNS5 (2021) molecular criteria, such as IDH mutation status, that distinguish IDH-wild-type glioblastoma from IDH-mutant grade-IV astrocytoma. After excluding IDH-mutant grade-IV cases from the CGGA cohorts, the classification AUROCs increased to 0.906 in CGGA-325 and 0.872 in CGGA-693, with a Cox risk-score hazard ratio of 8.57 ( = 1.4 × 10) and log-rank = 1.4 × 10 retained on the CNS5-aligned cohort. The methodological contributions introduced in this study, namely, the topology-aware hypergraph candidate pool construction, the rough set combinatorial reduct selection, the fixed-reference single-sample normalisation protocol, and the nested validation regime combining bootstrap optimism correction with cross-platform DeLong testing, are platform agnostic and directly applicable to future CNS5-aligned cohorts as such resources become publicly available, supporting the prospective re-derivation of molecularly defined glioma signatures within the integrated histopathological and molecular frameworks of contemporary neuro-oncology. - Source: PubMed
Publication date: 2026/05/12
Akgüller ÖmerBalcı Mehmet AliCioca Gabriela - Porcine reproductive and respiratory syndrome (PRRS) is a major economic burden to the global swine industry. Here, we identify the endoplasmic reticulum (ER) translocon component SSR4 as a critical host factor co-opted by PRRSV. We demonstrate that the viral non-structural protein Nsp2 physically interacts with SSR4 via its PLP2 and hypervariable domains and selectively upregulates its expression during infection by prolonging its protein half-life. Functional studies revealed that SSR4 is a proviral factor essential for efficient PRRSV replication. Mechanistically, SSR4 is required for the full activation of the PRRSV-induced ER stress response, specifically modulating the PERK-eIF2α and IRE1α-XBP1 axes of the unfolded protein response. Notably, Nsp2 itself acts as a key inducer of ER stress and mediates the upregulation of SSR4, suggesting a potential feed-forward loop that sustains a virus-favorable ER environment. This relationship is finely balanced, as pharmacological disruption of ER homeostasis using either the inducer tunicamycin (TU) or the chemical chaperone 4-phenylbutyric acid (4-PBA) potently inhibited viral replication. Importantly, TU and another inducer, dithiothreitol, exhibited potent, broad-spectrum antiviral activity against multiple PRRSV genotypes in both cell lines and primary porcine alveolar macrophages. Our study delineates a novel pathogenesis model where PRRSV Nsp2 hijacks SSR4 to engineer a proviral ER stress niche. The Nsp2-SSR4-ER stress axis represents a promising target for the development of broad-spectrum antiviral strategies against PRRS.IMPORTANCEThis study provides significant insights into porcine reproductive and respiratory syndrome virus (PRRSV) pathogenesis by identifying a novel and specific virus-host interface. We demonstrate that PRRSV, through its Nsp2 protein, hijacks a specific component of the host endoplasmic reticulum (ER) translocon SSR4 to orchestrate a tailored ER stress response conducive to viral replication. This mechanism is distinct from a general disruption of the TRAP complex, highlighting a precise viral strategy. Furthermore, the finding that pharmacological agents, which dysregulate this hijacked pathway-particularly ER stress inducers-act as potent, broad-spectrum antivirals challenges the conventional view of ER stress as a uniformly host-protective response. Our work not only uncovers a key molecular determinant of PRRSV replication but also validates the Nsp2-SSR4-ER stress axis as a promising and novel target for the development of much-needed, broad-spectrum therapeutic interventions against this economically devastating swine pathogen. - Source: PubMed
Publication date: 2026/04/23
Li YingchaoGao HongyanLiu ZhongLu ManShen YangXing YajingWang YuWu XiaotongYang PingpingYuan HongjieHou YanmengCai YumeiLi BaoquanXiao Yihong - L. (hops), belonging to the Cannabaceae family, is grown mainly for brewing, with 98% of global production directed to this sector. Moreover, large volumes of female cone residues are generated as by-products, representing a valuable source of bioactive compounds that can be valorized under green chemistry principles. This study aimed to extract bioactive compounds from hop cone residues sourced from craft breweries using ultrasound-assisted (EH-UA) and microwave-assisted (EH-MA) extraction methods. Hydroalcoholic extracts (70%) were analyzed for chemical composition, antioxidant, antimicrobial, antiproliferative, nitric oxide (NO)-production inhibition, and photoprotective activities. GC-MS identified 32 compounds in EH-MA and 30 in EH-UA, including terpenes, sesquiterpenes, oxygenated sesquiterpenes, and fatty acids. Both extracts demonstrated strong antioxidant activity in cell-based (TBARS, OxHLIA) and chemical (DPPH, ABTS, FRAP) assays, particularly EH-MA. Significant antibacterial activity was observed, especially against , , and (MIC 1-10 mg/mL), as well as antifungal activity against (MIC 2-2.5 mg/mL). Selective antiproliferative activity was observed against tumor cell lines Caco-2 and MCF-7 (GI 25 μg/mL), without cytotoxicity toward nontumor cell lines Vero and PLP2 (GI > 400 μg/mL). All extracts inhibited the production of the inflammation mediator NO, with EH-MA showing the most potent effect (IC of 35 μg/mL), followed by EH-UA (IC of 55 μg/mL). Photoprotective potential was also demonstrated, with SPF values of 19 (EH-MA) and 18 (EH-UA). In conclusion, hop cone residues can yield multifunctional extracts with antioxidant, antimicrobial, antiproliferative, anti-inflammatory, and photoprotective activities, which support their sustainable upcycling for pharmacological, nutraceutical, and cosmetic applications. - Source: PubMed
Publication date: 2026/03/24
Reyes Giulia BoitoSantos Emylaine Pereira DosSantos Everton da SilvaGonçalves Laura CorreiaSilva Gabriela Catuzo CanonicoGazim Zilda CristianiGonçalves Regina Aparecida CorreiaOliveira Arildo José Braz dePinela JoséMandim FilipaPires Tânia C S PCardozo-Filho LucioCorrêa Rúbia Carvalho GomesGonçalves José Eduardo - Hepatic fibrosis, driven by oxidative stress and subsequent hepatocellular injury, represents a major worldwide health challenge. Pueraria lobata Radix, a traditional Chinese herb, contains polysaccharides with demonstrated hepatoprotective properties, though their mechanisms remain incompletely defined. - Source: PubMed
Publication date: 2026/03/27
Wu JiangpingYang ZhuChen LingzhiXu QiangbaoZhang YingyingYang YipingBeloved MabhenaZhan SumiaoCao WenLi ZihanWang GuodongLv QiuyueHan Jun - Medik., is a neglected wild edible fruit traditionally consumed in several European regions and adapted to diverse agroecological conditions. The present study aimed to provides a preliminary physico-chemical and characterization of the fruits collected in central Spain. The analysis focused on fatty acids, tocopherols, soluble sugars, organic acids, and phenolic compounds, alongside an evaluation of the antioxidant and antimicrobial capacity. The results indicate that this wild edible fruit is characterized by a high proportion of unsaturated fatty acids, particularly oleic (34%) and linoleic (30%) acids, and contain appreciable amounts of tocopherols (28 mg/100 g, fw). The presence of sorbitol stands out in this species as a soluble sugar (> 2 g/100 g, fw), and the organic acid profile revealed a balanced composition. A high total phenolic content was observed, characterized by hydroxybenzoic and hydroxycinnamic acids. In vitro assays demonstrate radical scavenging capacity and inhibitory activity against selected pathogenic bacterial and fungal strains. Furthermore, no in vitro cytotoxicity was evidenced in non-tumour hepatic cell lines (PLP2). These findings provide a baseline characterization of the nutritional and bioactive compounds profile of fruits, suggesting their revalorization for its consumption as part of the Mediterranean diet and as a potential technological functional ingredient, although further studies on bioavailability and in vivo efficacy are required. - Source: PubMed
Publication date: 2026/04/10
García-Herrera PatriciaVega Erika NAlonso-Esteban José IgnacioTardío JavierMolina Adriana KMandim FilipaPires Tânia C S PBarros LillianPérez-Rodríguez María LuisaCámara MontañaFernández-Ruiz VirginiaMorales Patricia