K1C20_HUMAN KRT20 ELISA tesk kit
- Known as:
- K1C20_HUMAN KRT20 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen15982
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- K1C20_HUMAN KRT20 ELISA tesk kit
Related genes to: K1C20_HUMAN KRT20 ELISA tesk kit
- Gene:
- KRT20 NIH gene
- Name:
- keratin 20
- Previous symbol:
- -
- Synonyms:
- CK20, K20, MGC35423
- Chromosome:
- 17q21.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-02-04
- Date modifiied:
- 2016-03-09
Related products to: K1C20_HUMAN KRT20 ELISA tesk kit
Related articles to: K1C20_HUMAN KRT20 ELISA tesk kit
- Consensus molecular classes of urothelial carcinoma (UC) include basal/squamous (Ba/Sq), luminal papillary (LumP), luminal nonspecified (LumNS), luminal unstable (LumU), stroma-rich, and neuroendocrine (NE)-like. We aimed to determine the consensus molecular classifications of micropapillary (MP), plasmacytoid (PC), and sarcomatoid (SM) subtypes of bladder UC and characterize their spatial transcriptomic profiles. - Source: PubMed
Publication date: 2026/08/25
Zhao TingNawrocki ColeXiong LinjieNieman Linda TSaylor Philip JBlute Michael LMiyamoto David TTing David TDahl Douglas MWu Chin-Lee - Microphysiological systems (MPS) can improve intestinal epithelial modelling by combining 3D topographical cues, extracellular matrix mechanics and controlled apical-basal access. We present the EnView system, an imaging-compatible microphysiological platform configured to integrate human colon-inspired crypt-scale topography, tunable matrix stiffness and independent perfusion of apical/luminal and basal/stromal compartments. Crypt-like structures were molded in an interpenetrating polyacrylamide/collagen type I hydrogel, generating soft and stiff matrices with bulk stiffness values of 3.8 ± 1.8 kPa and 26.2 ± 8.4 kPa. Molecular transport through the hydrogel was characterized using FITC-dextrans of increasing molecular weight, and the experimentally derived diffusion coefficients were implemented in a numerical diffusion model to estimate the time required for soluble mediators to reach defined regions of the epithelial interface. Human colonic epithelial Caco-2 cells were cultured under continuous microfluidic perfusion for up to 21 days. Cells colonized the patterned surface, formed polarized epithelial monolayers and displayed stiffness-dependent morphology, with a more columnar organization on softer matrices highlighting the importance of matrix stiffness on cell morphology. Relative to standard 2D Transwell® conditions, these cultures exhibited increased gene expression of enterocyte markers such as (fatty acid binding protein-1), (intestinal alkaline phosphatase), (cytokeratin-20) and (villin-1), indicating that the EnView environment supports better epithelial polarization and maturation-associated features compared with conventional Transwell® culture. Basal/stromal TNF-α stimulation induced an apical/luminal measurable epithelial IL8 secretion, demonstrating the capacity of the system for basal stimulation and apical sampling. Our results show the relevance of considering matrix stiffness when modelling the human colon epithelium. This innovative MPS, recapitulating 3D topography, tunable matrix stiffness and continuous microfluidic perfusion, represents a powerful platform for long term culture and imaging of epithelial constructs enabled by active microfluidic control of the apical/luminal and basal/stromal compartments. - Source: PubMed
Publication date: 2026/08/19
Rojas-Garcia DuvanHamel DimitriFoncy JulieHut MarieThibault ChristopheSambe DiorPefaure SandraQuaranta-Nicaise MurielOnfroy-Roy LaurianeMalaquin LaurentFerrand Audrey - Pediatric ureteropelvic junction obstruction (UPJO) is the leading cause of congenital obstructive uropathy. Experimental models have identified local urothelial remodeling in UPJO and implicated this remodeling as a protective adaptation to preserve kidney structural integrity and function. We investigated the expression of urothelial proteins in human urine and resected tissue specimens from children with UPJO in a pilot study. - Source: PubMed
Publication date: 2026/07/10
Jackson Ashley RGupta SudiptiKercsmar MacieFroehlich John WLee Richard SBecknell BrianChing Christina B - Hirschsprung disease (HSCR) is characterized by distal aganglionosis. Persistent postoperative dysmotility and enterocolitis suggest that intrinsic epithelial vulnerabilities may remain within the ganglionic bowel. We here aimed to define both the state of the epithelium and its metabolism in HSCR. - Source: PubMed
Publication date: 2026/07/07
Zhao YitongYang JielinLi BoLee DorothyXiong YiPierro AgostinoJiang Qian - BackgroundThis study assessed the potential utility of GATA3, cyclin D1, KRT20, and GLUT1 immunohistochemistry (IHC) markers for diagnosing renal epithelial tumors.MethodsA retrospective analysis was conducted on all eligible specimens ( = 95) diagnosed at our institution between 2014 and 2024. IHC was done and the staining intensity and distribution of selected IHC markers were assessed.ResultsAmong 95 specimens, 26% were clear cell renal cell carcinoma, 21% were chromophobe RCC, and 17% were papillary RCC. Less frequent subtypes included TFE3-rearranged RCC, clear cell papillary renal cell tumor (CCPRT), papillary renal neoplasm with reverse polarity (PRNRP), and eosinophilic solid and cystic RCC (ESC-RCC). Clear cell RCC and CCPRT were consistently positive for cyclin D1 and GLUT1, and negative for GATA3/KRT20. CCPRT showed strong, diffuse GLUT1, unlike the limited staining in papillary RCC. PRNRP was positive for GATA3, cyclin D1, and GLUT1, and negative for KRT20. While chromophobe RCC exhibited variable GATA3 and GLUT1 expression with weak to moderate cyclin D1 positivity, oncocytomas were strong cyclin D1 positive, with negative GATA3, KRT20, and GLUT1. All 3 ESC-RCC specimens were KRT20 positive.ConclusionsOur findings validate many previously reported observations and characterize adjunctive patterns of these less commonly used markers across renal tumor subtypes. It suggests GATA3 as a potential diagnostic tool for PRNRP, while GLUT1 demonstrates differential expression patterns for papillary RCC and CCPRT, aiding in distinguishing papillary renal tumors. Similarly, cyclin D1 appears to be a valuable adjunct in differentiating oncocytoma from chromophobe RCC. Also, KRT20 shows high specificity for ESC-RCC. - Source: PubMed
Publication date: 2026/06/25
Barakzai Muhammad AbrarArain Shoukat AliAfsar Nasir AliAlsouss Yara ObaedaAl-Hussain Turki