Rat TNF_alpha 1 mg
- Known as:
- Rat TNF_alpha 1 mg
- Catalog number:
- 1052-1000
- Product Quantity:
- 1 mg
- Category:
- -
- Supplier:
- Biovis
- Gene target:
- Rat TNF_alpha 1
Related products to: Rat TNF_alpha 1 mg
Related articles to: Rat TNF_alpha 1 mg
- The study aimed to optimize the processing conditions of Gardeniae Fructus with ginger juice (GFPG) and confirm its therapeutic effects and pharmacological mechanisms on cholestatic liver injury. Processing conditions were optimized using response surface methodology (RSM) and thermal analysis, focusing on geniposide content as a key active compound. Variables included processing time, moistening time, and the solid-liquid ratio. Optimal conditions were: ginger juice to Gardeniae Fructus ratio of 8:1 (w/v), processing temperature of 208 °C, moistening time of 3 hours, and processing time of 5 minutes. Pharmacological mechanisms were analyzed through network pharmacology, molecular docking, and experimental validation using alpha-naphthyl isothiocyanate (ANIT)-induced cholestatic liver injury in mice and lipopolysaccharide (LPS)-stimulated RAW264.7 cell models. In vivo, GFPG extract alleviated ANIT-induced cholestatic liver injury by improving liver function markers (AST, ALT, TBA, TBIL, DBIL) and modulating TLR4/NF-κB, FXR/PPAR-α, and PI3K/AKT/GSK-3β pathways. In vitro, it reduced LPS-induced production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β) through TLR4/NF-κB pathway inhibition. This study established optimal processing methods for GFPG using RSM and thermal analysis, providing robust quantitative parameters. GFPG demonstrated significant therapeutic effects in cholestatic liver injury models, indicating its potential as a candidate for developing treatments for cholestatic hepatitis. - Source: PubMed
Publication date: 2025/09/16
Lyu ChenziMeng XianglongWang JuanShao HaifengWang YangPark Yong-KiZhang ShuoshengJung Hyo Won - Sustained CD4+ T cell immunity is required for resolution of acute hepatitis C virus (HCV) infection but the response remains poorly characterized. Here, circulating CD4+ T cells with high PD-1 and ICOS co-expression were temporally associated with onset of virus control, seroconversion, and hepatitis in HCV-infected chimpanzees. Co-production of Tfh (IL-21, CXCL13) and Th1 (IFN-γ, TNF) cytokines after stimulation with HCV non-structural proteins demonstrated that the response was predominately Tfh1-like and virus-specific. Transcriptional analysis confirmed a Tfh1 lineage assignment. Effector-related genes such as ADGRG1 (GPR56), ZNF683 (Hobit), and KLRB1 (CD161) were also expressed. HCV-specific PD-1hiICOShi CD4+ Tfh1-like cells were enriched in liver, suggesting the potential for B and CD8+ T cell help at the site of virus replication. Most circulating and intrahepatic PD-1hiICOShi CD4+ Tfh1-like cells did not express CXCR5, and therefore resembled CXCR5-negative CXCL13-positive peripheral helper (Tph) cells that infiltrate tumors and tissues inflamed by autoimmunity. PD-1hiICOShi CD4+ cells also peaked after hepatitis A virus infection, but the response was accelerated by several weeks when compared with HCV infection. The PD-1hiICOShi phenotype, and temporal association between the peak response and ALT, may provide markers to guide human studies of CD4+ T cell immunity against HCV and other hepatotropic viruses. - Source: PubMed
Publication date: 2025/09/16
Blasczyk HeatherBremer William GPhelps Christopher CZhou YanBowen David GXu ZhaohuiLanford Robert EShoukry Naglaa HGrakoui ArashSkinner Nicole EWalker Christopher M - This study was aimed to evaluate the renoprotective effects of Myricetin at two dosages (50 and 100 mg/kg) within an experimental I/R injury model. The analysis focused on key immunological and oxidative stress pathways, including the regulation of pro- and anti-inflammatory cytokines, NF-κB and TLR4 signaling, as well as biomarkers of oxidative stress such as nitrate (NO), malondialdehyde (MDA), glutathione (GSH), nuclear factor (erythroid-derived 2)-like 2 (NRF2) and Heme oxygenase-1 (HO-1). - Source: PubMed
Publication date: 2025/09/16
Sezer MertBati Yusuf UmutYerli NazliKina SonerBaser LaleKaramese Selina Aksak - Acute lung injury (ALI), especially burn-induced cases complicated by secondary infections and hyperinflammation, remains challenging to treat. This study developed 4-mercaptobenzoic acid (MPBA)-modified copper nanozymes (CuMPBA) to simultaneously combat bacterial infections and toxin-triggered immune overactivation. CuMPBA binds bacterial surface polysaccharides via boronate ester bonds, neutralizing lipopolysaccharides (LPS) and lipoteichoic acid (LTA). It demonstrates dual enzymatic activity: peroxidase (POD)-like and glutathione peroxidase (GPx)-like activities for antimicrobial effects. Additionally, CuMPBA disrupts Streptococcus pneumoniae (Sp) metabolism by interfering with thiamine utilization, amino acid synthesis, DNA processes, and energy production. In burn-ALI mice with secondary pneumonia, CuMPBA restored lung architecture, suppress TNF-α/IL-1β/IL-6 levels, and modulated inflammatory pathways by activating Nrf2 while inhibiting NF-κB. These synergistic mechanisms (precise bactericidal action combined with toxin neutralization) establish CuMPBA as a promising dual-target therapeutic strategy for complex burn-associated ALI. The multifunctionality of nanozymes addresses both infection control and inflammation resolution, offering new potential for managing this severe pathological condition. - Source: PubMed
Publication date: 2025/09/16
Ge HaojieWang MinXie HaijiaoChen Xu-LinWang Xianwen - Optical modulation of immune responses via nanomaterials has emerged as a promising approach in cancer immunotherapy, but challenges in achieving precise activation with minimal phototoxicity persist. In this study, we developed a galactose-functionalized Au-S (2ATP)/polyaniline (PANI)-based glycopolymer nanoparticle (Au/2ATP@PGlyco NP) to enable multivalent galactose-based biostimulation of M2-like macrophages and hot electron/hole-elicited reactive oxygen species (ROS) generation for synergistic macrophage reprogramming. The 2ATP@PANI-based shell not only facilitated light-driven charge transfer to enhance O generation but also provided Raman-active properties that enabled single-cell visualization of phenotypic transitions toward the M1 phenotype through the NF-κB and STAT-1-mediated pro-inflammatory signaling. Such light-driven reprogrammed M1-like macrophages with Au/2ATP@PGlyco NP effectively induced apoptosis in MB49 bladder cancer cells through phagocytosis and the release of TNF-α and IL-12, resulting in a potent antitumor effect. This research highlights a new nanobiophotonics platform that holds potential for advancements in macrophage modulation within cancer immunotherapy. - Source: PubMed
Publication date: 2025/09/16
Cheng Ting-YuChang Li-ChanYang Li-XingWu Sz-SyuanChin Yu-ChengChen Ya-JyunChia Zi-ChunSu Wen-PinHuang Chih-Chia