Rat TNF_alpha 1 mg
- Known as:
- Rat TNF_alpha 1 mg
- Catalog number:
- 1052-1000
- Product Quantity:
- 1 mg
- Category:
- -
- Supplier:
- Biovis
- Gene target:
- Rat TNF_alpha 1
Related products to: Rat TNF_alpha 1 mg
Related articles to: Rat TNF_alpha 1 mg
- Ischemic stroke is a leading cause of death and disability, yet effective pharmacological interventions remain limited. Ginsenoside Rb1, a principal bioactive compound of , has demonstrated neuroprotective activity, but its metabolic mechanisms remain incompletely defined. Here, we combined lipid metabolomics with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the spatially resolved metabolic effects of Rb1 in a rat model of cerebral ischemia/reperfusion injury. Rb1 treatment significantly reduced infarct volume and improved neurological outcomes. Metabolomic profiling revealed that Rb1 reversed ischemia-induced disturbances in the glycerophospholipid and amino acid metabolism, while MSI demonstrated recovery of phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine distributions in the ischemic cortex. These metabolic improvements were strongly correlated with reduced levels of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and oxidative stress marker malondialdehyde, along with increased superoxide dismutase activity. Rb1 also preserved blood-brain barrier integrity by enhancing the expression of tight-junction proteins ZO-1 and Occludin. Together, these findings indicate that Rb1 confers neuroprotection through metabolic reprogramming linked to anti-inflammatory and antioxidant actions, and they highlight the value of integrating metabolomics with MSI to elucidate spatially defined drug mechanisms in the brain. - Source: PubMed
Publication date: 2026/05/08
Li MuziQian ShiyanQian LingkeCui GuoqianTan ShuoyanGuo WenboChen ShengshuangZheng GuoqingLiao JieFan Xiaohui - Autoimmune thyroiditis (AIT), caused by immune-mediated thyroid dysfunction, lacks effective dietary interventions. This study investigates the therapeutic potential of a ketogenic diet (KD) in a murine model of iodine-induced AIT. - Source: PubMed
Publication date: 2026/05/08
Luo YueGao HaoWang MengzhenWang ZhiminJin ZheSong NanLiu ZiyuYang Xiao - This study aimed to evaluate whether a ketogenic diet (KD) mitigates behavioral, metabolic, and peripheral tissue alterations in a rat model of propylthiouracil (PTU)-induced hypothyroidism. - Source: PubMed
Publication date: 2026/05/08
Akkaya Erhan CanerIlgin RabiaKızıldağ ServetArgon AsumanHoşgörler Ferda - Septic arthritis is a severe infection caused by bacteria directly invading the synovial joints. It leads to inflammatory damage to articular cartilage and may result in significant functional impairment, with a high recurrence rate. The rapid spread of antibiotic-resistant bacterial strains has further exacerbated the threat to human health, particularly in septic arthritis cases. Based on the antibacterial activity and regenerative properties of pancreatitis associated protein (PAP), this study aims to investigate the therapeutic potential of exogenous recombinant PAP for treating methicillin-resistant Staphylococcus aureus (MRSA)-induced septic arthritis. The prepared PAP exhibited favorable bactericidal effects against both Staphylococcus aureus (S. aureus) and MRSA, with a dose-dependent manner. The antibacterial efficacy in the high-dose group was comparable to that of vancomycin. Additionally, 10 nM PAP significantly promoted the proliferation of RAW264.7 macrophages and enhanced their resistance to oxidative stress. Intra-articular injection of PAP accelerated the recovery from MRSA-induced septic arthritis by alleviating joint swelling, clearing the bacteria within the joint cavity, and mitigating inflammation. Transcriptomic results indicated that PAP administration significantly downregulated the expression of inflammatory markers and slightly increased the proportion of monocytes/macrophages. Further studies on the immunomodulatory effects of PAP revealed that it attenuated lipopolysaccharide (LPS)-induced M1 polarization and markedly diminished the expression of pro-inflammatory cytokines, including TNF-α, IFN-γ, and IL-6. Meanwhile, PAP partially induced M2 polarization of macrophages and elevated anti-inflammatory IL-4 levels. In conclusion, PAP exhibited antibacterial, tissue repair, and immunomodulatory activities, highlighting its potential as a therapeutic candidate for septic arthritis. - Source: PubMed
Publication date: 2026/05/08
Yu LutingLin GuanghaoJiang ShenglongLi YuanxinRen LiliChen Guoguang - The safety of live probiotics remains debated due to concerns regarding their viability and functional efficacy under harsh in vivo and in vitro conditions. Inflammatory bowel disease (IBD) is a complex disease stemming from various factors. It has been reported that probiotics shows anti-inflammatory function. This study evaluated the immunomodulatory and anti-inflammatory properties of heat-killed Lactobacillus strains (a form of postbiotics). Because viable Loigolactobacillus coryniformis NA-3 exhibited lower in vitro viability than Lactiplantibacillus plantarum NA, we first evaluated the phagocytic activity and TLR2-mediated immunoregulatory effects of heat-killed L. coryniformis NA-3 in RAW 264.7 cells, and conducted a preliminary comparative assessment of both live and heat-killed L. coryniformis NA-3 in murine models. We further investigated two distinct heat-killed strains: L. coryniformis NA-3 and Lactiplantibacillus plantarum NA. Heat-killed L. coryniformis NA-3 induced TLR2-dependent immunomodulatory effects in vitro and showed effects comparable to those of its live counterpart in vivo under the conditions tested. Both heat-killed strains were associated with changes in inflammatory markers, including decreased levels of pro-inflammatory cytokines (IL-6, TNF-α) and increased levels of anti-inflammatory mediators (IL-4, IL-10, TGF-β) in mice. In addition, they were associated with alterations in antioxidant parameters, including changes in SOD, GSH-Px, GR, NQO1, HO-1, Nrf2, and MDA levels, as well as increased expression of the tight junction proteins claudin-1 and occluding. Observations from this study are consistent with the potential involvement of the TLR2/NF-κB p65 signaling pathway. These findings highlight the potential of heat-killed probiotics as safe and effective alternatives to live strains in immune regulation and anti-inflammation. - Source: PubMed
Publication date: 2026/05/08
Xu XiaoqingTian DandanQiao YuPeng QingRan ChaoYang YalinZhang ZhenYao YuanyuanDing QianwenZhou Zhigang