ARGI1_MOUSE Arg1 ELISA tesk kit
- Known as:
- ARGI1_MOUSE Arg1 Enzyme-linked immunosorbent assay test tesk reagent
- Catalog number:
- gen15017
- Product Quantity:
- 1
- Category:
- Peptides
- Supplier:
- Other suppliers
- Gene target:
- ARGI1_MOUSE Arg1 ELISA tesk kit
Related genes to: ARGI1_MOUSE Arg1 ELISA tesk kit
- Gene:
- ARG1 NIH gene
- Name:
- arginase 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 6q23.2
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2016-10-05
Related products to: ARGI1_MOUSE Arg1 ELISA tesk kit
Related articles to: ARGI1_MOUSE Arg1 ELISA tesk kit
- Pneumoconiosis is a common occupational lung disease with unclear pathogenesis and no effective targeted therapies. Emerging evidence suggests that daphnetin is a promising therapeutic candidate for silicosis, yet its efficacy targets remain poorly characterized. In this study, we propose and implement a multi-layered target identification strategy that integrates transcriptomics, clinical metabolomics, public database mining, an artificial intelligence (AI)-based target prediction model, and tissue-based thermal proteome profiling (Tissue-TPP) technology. This strategy enables stepwise elucidation, from pneumoconiosis-related targets to daphnetin efficacy targets and ultimately to direct targets. Using this approach, we validated nicotinamide adenine dinucleotide phosphate hydrogen (NAD(P)H) quinone oxidoreductase 1 (NQO1) and 5-oxoprolinase (OPLAH) as direct targets of daphnetin, and identified Kelch-like ECH-associated protein 1 (KEAP1), nuclear factor erythroid 2-related factor 2 (NRF2), and arginase 1 (ARG1) as indirect targets. Notably, OPLAH is reported here as a direct target of daphnetin for the first time. Furthermore, the binding of daphnetin to NQO1 and OPLAH helps explain its effects on cellular thiol levels. Using a thiol-responsive probe, we observed that daphnetin significantly increased intracellular thiol levels and reduced silica-induced reactive oxygen species (ROS) accumulation in both A549 and THP-1 cells, thereby contributing to its antioxidant effects. Additionally, overexpression (OE) of OPLAH significantly increased reduced glutathione (GSH) levels and alleviated silica-induced epithelial-mesenchymal transition (EMT). Conversely, OPLAH knockdown partially inhibited the efficacy of daphnetin. Together, these findings clarify the anti-fibrotic mechanism of daphnetin, support its therapeutic potential for silicosis, and provide a practical framework for the target discovery of natural products (NPs). - Source: PubMed
Publication date: 2026/04/02
Wang LingMa Bo-YanJiang Sheng-PingWei Shu-TingQin Xue-MeiLi Zhen-Yu - Sulfated polysaccharides from red seaweeds are emerging as versatile bioactive macromolecules. Here, we report that funoran-type galactans from (GV) function as immunometabolic reprogrammers, orchestrating inflammation resolution through targeted remodeling of amino acid and fatty acid metabolism in macrophages, potentially via receptor-mediated pathways. Cold and hot water extraction yielded structurally distinct fractions (360-3006 kDa) characterized by NMR as sulfated galactans with κ-carrageenan motifs and variable sulfation (6.6-21.5%). In RAW264.7 macrophages, fractions GV-1A and GV-2A induced an anti-inflammatory metabolic state characterized by arginine accumulation (from 4.61% to 7.06% of the total amino acid pool), reduced ornithine levels, and complete the elimination of pro-inflammatory myristate (14:0), while upregulating phagocytosis (149% of control). In a carrageenan-induced paw edema model, GV-1A and GV-2A dose-dependently upregulated pro-resolution markers (, , , , HO-1), outperforming the standard drug meloxicam. While the arginine accumulation suggests functional arginase inhibition, the upregulation reflects a transcriptional response in a complex tissue environment. We propose that these findings collectively support a 'resolution-phase metabolic phenotype', a working model whereby GV polysaccharides reprogram macrophage metabolism toward inflammation resolution through context-dependent mechanisms. These findings establish polysaccharides as metabolically active immunomodulators that promote inflammation resolution, with the potential for applications in inflammatory diseases and as a pharmaceutical platform for drug development. - Source: PubMed
Publication date: 2026/08/27
Premarathna Amal DDziubinska-Kuehn Katarzyna MSaravanan MuthupandianSooäär AntiReile IndrekAhmed Tamer A EHincke Maxwell TTuvikene Rando - Macrophages play a critical role in regulating kidney injury and repair. Arginase 1 (ARG1), a key enzyme in L-arginine metabolism, is predominantly expressed in macrophages in injured kidneys. Polyamines derived from L-arginine metabolism promote the resolution of tissue injury. Follistatin-like 3 (FSTL3) is a secreted protein that acts as an antagonist of activins. However, its role in the kidney remains unknown. - Source: PubMed
Publication date: 2026/09/23
Xing FeiXiang LiYan JingruChu XiaoyangWang YangKong YonglunQin JinzhongJiang PeiyongSun HaoZou HequnLan Hui-YaoHuang YuZhang YuCai ZongweiXia Yin - Blastocyst implantation is associated with quasi-inflammatory reactions in the endometrium, which are resolved during the establishment of the definitive placenta. Quasi-inflammatory processes have also been documented in pregnant pigs, even though the blastocyst only attaches to, rather than implants into the endometrium. We asked what processes lead to the resolution of attachment-associated inflammation. In wound healing the transition towards an anti-inflammatory state includes the transition from M1 to M2 polarized macrophages. In order to determine whether this scenario applies also to the peri-attachment porcine uterus, we produced a series of bulk transcriptomes spanning days 13 to 25 of gestation. We found slower changes in the transcriptome between D20 and D25 than prior to D20, suggesting a turning point in pregnancy-related endometrial reprogramming. This period corresponds to the firm attachment of trophectoderm to the uterine epithelium and the cessation of IFNG signaling from the blastocyst. We found increased expression of genes implicated in resolution of inflammation and M2 polarization such as ARG1, MRC1, CD86, TGFb1 and IL10, and an increase in expression of HGPD, the enzyme metabolizing prostaglandins. While immunoreactivity for ARG1 was found in putative macrophages, other M2 markers were localized to non-immune cells: MRC1 in fibroblast-like stromal cells, CD86 on trophoblast cells, and IL10 in endometrial epithelia. CD86 undergoes trogocytosis into the luminal epithelium by D25. These results suggest that intrauterine immune regulation is decoupled from that of the rest of the body by engaging non-immune cells as anti-inflammatory mediators during the peri-attachment period of porcine pregnancy. - Source: PubMed
Publication date: 2026/09/23
Wagner Günter PMinela ThaináRoss AlexandriaEngelhardt JanBazer Fuller WJohnson Gregory A - Chronic cutaneous wounds remain a major clinical challenge due to a persistent pro-inflammatory microenvironment characterized by unresolved inflammation and dysregulated macrophage polarization. Emerging "immune-instructive" biomaterials offer a promising strategy to actively modulate host immune responses rather than providing mere structural support. In this study, we performed a comparative screening of representative traditional Chinese medicine (TCM)-derived monomers, identifying baicalin (BAL) as the most potent regulator of macrophage polarization. To ensure localized and sustained bioactivity, BAL was encapsulated into mesoporous silica nanoparticles (MSNs) and subsequently integrated into electrospun poly(ε-caprolactone)/silk fibroin (PCL/SF) nanofibrous membranes to construct a hierarchical bioactive system (BA@MSN/PCL/SF). Mechanistic investigations combining network pharmacology, molecular docking, and pharmacological inhibition revealed that BAL directs macrophage polarization toward a pro-regenerative M2 phenotype the PI3K/AKT/GSK3β signaling axis. , the composite membranes exhibited excellent cytocompatibility and effectively modulated inflammatory responses. In a murine full-thickness wound model, BA@MSN/PCL/SF membranes significantly accelerated wound closure, enhanced granulation tissue formation, and promoted high-quality collagen deposition and skin appendage regeneration. These therapeutic effects correlated with a pronounced shift in the local immune microenvironment, evidenced by increased ARG1 and decreased iNOS macrophage populations. Our findings demonstrate that integrating potent natural immunomodulators with controlled-release hierarchical scaffolds provides a sophisticated strategy for engineering immune-instructive wound dressings, offering a mechanistic basis for modernizing traditional therapies in regenerative medicine. - Source: PubMed
Publication date: 2026/09/23
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