Rat Anti-Mouse ABCA2, FITC-labeled
- Known as:
- Rat Antibody toMouse ABCA2, fluorecein-labeled
- Catalog number:
- 129-10008
- Product Quantity:
- 100
- Category:
- -
- Supplier:
- Ray Biotech
- Gene target:
- Rat Anti-Mouse ABCA2 FITC-labeled
Related genes to: Rat Anti-Mouse ABCA2, FITC-labeled
- Gene:
- ABCA2 NIH gene
- Name:
- ATP binding cassette subfamily A member 2
- Previous symbol:
- ABC2
- Synonyms:
- -
- Chromosome:
- 9q34.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-03-16
- Date modifiied:
- 2016-10-05
Related products to: Rat Anti-Mouse ABCA2, FITC-labeled
Related articles to: Rat Anti-Mouse ABCA2, FITC-labeled
- The ATP-binding cassette subfamily A member 2 (ABCA2) gene encodes an ABC transporter protein. Biallelic loss-of-function variants in ABCA2 have been associated with an intellectual disability disorder. We aimed to delineate the phenotypic spectrum of individuals with monoallelic (MV) or biallelic (BV) variants in the ABCA2 gene. We collected clinical data via questionnaires and literature review. The ABCA2 protein was constructed using homology modeling. Untargeted plasma metabolomics was performed at Metabolon Inc. A docetaxel toxicity cell culture model system was used to study the effect of plasmid-encoded ABCA2 and four ABCA2 variants (p.Asp615Glu, p.Phe754Ser, p.Ile786del, p.Arg926Trp) on cell viability. Seventeen individuals with seventeen candidate ABCA2 variants were identified (seven MV, ten BV). Protein modeling predicted a likely significant impact for eight of 13 assessed variants. In cell viability assays, enforced expression of wild type ABCA2 reduced viability in docetaxel-exposed cells. In contrast, none of the four variants affected viability, suggesting a loss or a marked reduction in transporter function. Untargeted metabolomics of three samples from two individuals showed a trend toward lower levels of polyunsaturated acylcarnitines. This study describes 17 individuals with candidate variants in the ABCA2 gene. The cell viability assays revealed that four variants (p.Asp615Glu, p.Phe754Ser, p.Ile786del, p.Arg926Trp) had an altered ability to function as a transporter compared to wild type ABCA2. Further studies are needed to clarify the pathomechanism of ABCA2 and the clinical significance of the variants. - Source: PubMed
Publication date: 2026/06/25
Oja Kaisa TReinson KaritIlisson MihkelÖrd DaimaWojcik Monica HSeaby Eleanor GWittmann Bryan MKennedy Adam DDeBalsi KarenZweier ChristianeVasileiou GeorgiaAlkhawaja Issam AWirth ThomasSyrbe SteffenPlatzer KonradAlyamani SuadAlkuraya Fowzan SRos-Pardo DavidMarcos-Alcalde IñigoGómez-Puertas PaulinoVidailhet MarieSu KeFan ShaohuaMa YuWang YiRenieri AlessandraMaria Pinto AnnaAlmadhoun FarahPetersen Andrea KMagnussen KariBodamer OlafAlanzi TalalMohamed SararAlrabee HadeelAlhaddad BaderBakur KhadijahFleischer NicoleBegtrup AmberÖrd TõnisPajusalu SanderÕunap Katrin - The gut microbiome is a critical regulator of host health, but how it mediates the therapeutic effects of drugs targeting neurodegenerative diseases like diabetic cognitive impairment (DCI) is unclear. Here, we investigated whether the neuroprotective effects of the GLP-1 agonist semaglutide (SE) are linked to its modulation of the gut-brain axis. - Source: PubMed
Publication date: 2026/03/26
Qi LiqinKang HuiminLi XiaofenWang LijingLin YinchenZhan MenglanZeng FeihuiXiao ZhiwenLiu XiaoyingChen ZhouLiu Libin - Fat deposition plays a crucial role in regulating the production performance and meat quality of broilers. Although the heterogeneity of mammalian adipocytes has been extensively studied, research on the molecular mechanisms underlying differences in lipid droplet accumulation in avian adipocytes remains limited. This study confirmed a significant positive correlation (R > 0.81, < 0.001) between the SSC signal and lipid droplet content via fluorescence staining of lipid droplets, Oil Red O staining, and triglyceride (TG) quantification. Based on this, a label-free sorting strategy using SSC signals was established to sort differentiated chicken preadipocytes, obtaining high lipid droplet (H) and low lipid droplet (L) subpopulations, which were subsequently subjected to transcriptome sequencing and differential gene expression (DEG) analysis, followed by GO and KEGG enrichment analysis. The results indicated no significant differences in the expression of adipogenesis marker genes (, , , , ) between the high lipid droplet (H) and low lipid droplet (L) groups, suggesting that both groups are at similar stages of differentiation. KEGG analysis revealed that both the H vs. NC and L vs. NC comparisons were enriched in common pathways, including the PPAR signaling pathway, ECM-receptor interaction, focal adhesion, cytokine-receptor interaction, and calcium-Apelin signaling pathway, suggesting that both groups of cells had activated the adipogenesis program. GO analysis showed that, in both H vs. NC and L vs. NC comparisons, differentially expressed genes (DEGs) were enriched in biological processes (BPs) related to cell adhesion, nucleosome assembly, chromatin remodeling, and receptor activity, as well as cellular components (CCs) such as the extracellular matrix, cytoskeleton, and nucleosome organization, indicating extensive gene reprogramming and activation of signaling transduction during differentiation. In the H vs. L comparison, enriched pathways included ABC transporters, ECM-receptor interaction, focal adhesion, gap junctions, microtubule-related processes, and neuroactive ligand-receptor interactions, involving lipid transmembrane transport, cytoskeleton stabilization, and signal transduction regulation, suggesting that high lipid droplet cells are more mature in lipid droplet transport, storage, and homeostasis maintenance. GO enrichment results further supported this conclusion, as H vs. L specifically enriched processes related to microtubule-related processes, cell cycle, and redox reactions (BPs), as well as chromosome organization, cytoskeleton, and motor activity (CC/MF), indicating that high lipid droplet cells maintain lipid droplet fusion and metabolic homeostasis via enhanced microtubule transport and antioxidant regulation. Differential gene analysis revealed that the L group upregulated genes associated with fatty acid synthesis and elongation (, , , , ), cholesterol and isoprenoid biosynthesis (, , , , , , ), and fatty acid oxidation (, , , ), reflecting a metabolic characteristic of concurrent lipid synthesis and mobilization; the H group, conversely, upregulated genes associated with lipid droplet formation and storage (, , , , ), lipid transport (, , , , ), and antioxidant defense (, , ), exhibiting a storage and homeostasis-oriented metabolic state. In the NC, L, and H groups, the expression of five genes-, , , , and -showed a gradual increase, suggesting that these genes were associated with preadipocyte differentiation and lipid droplet deposition. In summary, although the high and low lipid droplet subpopulations of chicken preadipocytes exhibit similar differentiation states, they form distinct metabolic orientations. The L group is characterized by active lipid synthesis, fatty acid oxidation, and membrane lipid remodeling, while the H group predominantly features lipid droplet storage, lipid transport, and antioxidant homeostasis. This study highlights the molecular mechanisms underlying the metabolic heterogeneity of avian adipocytes and provides a theoretical basis for poultry fat deposition regulation and genetic improvement. - Source: PubMed
Publication date: 2026/03/12
Wang BoyuLi YantaoWang YakeChen JiayiWang JialiLi XiaopingLi Zhenhui - BACKGROUND: Pyrethroid resistance continues to undermine malaria vector control across Africa, particularly in agricultural hotspots where insecticide pressure is high. However, the molecular mechanisms enabling Anopheles gambiae to survive extreme pyrethroid doses remain poorly understood. This study investigates transcriptomic responses, allele‑frequency shifts, and selection signatures associated with high‑ permethrin resistance intensity in An. gambiae from Mangoum, Cameroon. RESULTS: RNA‑seq analyses revealed strong overexpression of detoxification genes in both unexposed and permethrin‑exposed field mosquitoes. The UDP‑glycosyltransferase UGT308G1 showed the highest expression levels (FC = 105.1 in C–S; 50.8 in R–S). Within the P450 family, members of the CYP6Z cluster were markedly overexpressed, notably CYP6Z3 (FC = 68.8 in C–S; 41.4 in R–S), CYP6Z2 (FC = 29.0; 17.2), and CYP6Z1 (FC = 12.3; 6.0). Additional detoxification genes such as CYP9K1 (FC = 11.3; 4.7), CYP6M2 (FC = 8.5; 3.8), and the cuticle‑associated CYP4G17 (FC = 2.9; 2.5) were strongly upregulated. Increasing permethrin doses (1 × , 5 × , 10 ×) induced further upregulation of oxidative‑stress, mitochondrial, and translational pathways. Gene‑level population‑genetic metrics revealed strong selective sweeps at VGSC and across the rp1 region. High‑frequency nonsynonymous variants in CYP6AA1 (Ser395Thr), CYP6AA2 (Asn327Asp), CYP6P1 (Ile168Val and Leu374Met), and the validated marker Glu205Asp-CYP6P3 were nearly fixed in field populations and absent in lab susceptible strain, consistent with long‑term selection and hitchhiking. Additional metabolic variants in CYP12F2, UGT49A3, UGT308A2, and ABCA2 occurred at moderate to high frequencies and distinguished resistant from susceptible genetic backgrounds. RNAi‑mediated silencing of CYP6Z1, CYP6Z2, CYP6Z3, and CYP6Z4 significantly increased mortality under permethrin and alpha‑cypermethrin 1X, 5X and 10X exposure, confirming the functional involvement of the CYP6Z family in resistance escalation. CONCLUSIONS: High pyrethroid resistance intensity in An. gambiae arises from strong target‑site selection coupled with extensive metabolic adaptation. The convergence of transcriptomic signals, allele‑frequency patterns, and RNAi assays highlights the CYP6Z genes cluster, together with key P450, UGT, and ABC variants, as central components of a polygenic resistance architecture. These findings underscore the need to integrate metabolic markers into resistance surveillance to detect and manage escalating pyrethroid resistance. - Source: PubMed
Publication date: 2026/03/23
Tepa ArnaudKouamo MersimineKengne-Ouafo Jonas ATchouakui MagellanPieme Constant AWondji Charles S - ATP-binding cassette (ABC) transporters regulate xenobiotic efflux, oxidative stress responses, and blood-brain barrier (BBB) homeostasis. Dysregulation of transporters such as ABCC1, ABCB1, and ABCA2 has been linked to neuropsychiatric disorders, yet their expression patterns in schizophrenia and their modulation by antipsychotic treatment remain unclear. This study investigated longitudinal changes in the expression of these genes in schizophrenia patients before and after antipsychotic therapy, compared with healthy controls. - Source: PubMed
Publication date: 2025/12/09
Çevik Filiz EkimGuzel Tanoglu EsraCakmur Kadriye NurEsen Muhammed FevziUzun Fatma RumeysaErkiran Murat