Rat Anti-Mouse CD90 Thy-1.2
- Known as:
- Rat Antibody toMouse CD90 Thy-1.2
- Catalog number:
- 128-10061-2
- Product Quantity:
- 1 mg
- Category:
- -
- Supplier:
- Ray Biotech
- Gene target:
- Rat Anti-Mouse CD90 Thy-1.2
Related genes to: Rat Anti-Mouse CD90 Thy-1.2
- Gene:
- THY1 NIH gene
- Name:
- Thy-1 cell surface antigen
- Previous symbol:
- -
- Synonyms:
- CD90
- Chromosome:
- 11q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2015-07-22
Related products to: Rat Anti-Mouse CD90 Thy-1.2
Related articles to: Rat Anti-Mouse CD90 Thy-1.2
- We previously determined that 2-Methylglutamate (2MeGlu) and 4-aminopentanoic acid (4APA) have neurochemical properties of glutamatergic and GABAergic false neurotransmitters (FNTs); here, we tested whether their activity impacts mouse models with excitation-inhibition (E-I) imbalance. We first screened racemic agents using models caused by E-I imbalance; rac-2MeGlu, but not rac-4APA, suppressed 81% of excitotoxicity in hippocampal slices and increased survival by 105% in Aldh5a1 mice. Enantiomers with the least receptor activity were further tested in more complex models. R-4APA (50 mg/kg) worsened startle behaviors in the Shank3 autism model while S-2MeGlu (50 mg/kg/d over 19 days) improved motor performance by 77% in MPTP-treated mice without changing dopaminergic neurotoxicity; neither agent improved motor function in a human α-synuclein overexpressing mouse. S-2MeGlu (10 mg/kg/d for 8 weeks), but not R-4APA, reversed the spatial working memory deficit in T41 (Thy-1 hAPP ) mice without significantly changing Aβ plaque density. Single-nucleus transcriptomics following the same chronic exposures in WT mice yielded positively enriched pathways related to protein handling and synaptic regulation in excitatory neurons with S-2MeGlu; R-4APA caused metabolic pathway negative enrichments in multiple cell types. Our data reveal distinct behavioral and transcriptomic impacts of S-2MeGlu and R-4APA and further support S-2MeGlu as a glutamatergic FNT. - Source: PubMed
Publication date: 2026/05/20
Perna AmaliaZhao JingGajera Chandresh RSchonemann MarcusSaw Nay LShamloo MehrdadMontine Kathleen SGarofalo Albert WMontine Thomas J - Metabolic dysfunction contributes to glaucoma progression, including through substrate availability and the presence and concentration of substrate transporters. Observations of metabolic substrate transporter loss in glaucoma, including loss of monocarboxylate transporter-2 (MCT2), have suggested there are serious implications for metabolic dysfunction on the health and survival of retinal ganglion cells (RGCs). In this study, we investigate whether MCT2 is necessary and sufficient for RGC survival and after ocular hypertension (OHT). We used an inducible conditional knockout (KO) mouse to remove MCT2 in Thy1-positive RGCs, then assessed RGC survival and function after OHT. MCT2 KO alone did not affect RGC density but did significantly reduce pattern electroretinogram amplitude. Upregulation of MCT1, MCT4, and GLUT3 transporters occurred as a result of MCT2 KO, suggesting that RGCs employ compensatory measures to meet their metabolic needs. Introducing oral nicotinamide (500 mg/kg/day) to test its ability to offset potential energy substrate insufficiency from MCT2 KO showed that nicotinamide was protective of RGC density for the MCT KO group but did not preserve RGC density or function for MCT2 KO + OHT. These data indicate RGCs are able to undergo compensatory adaptation to MCT2 KO with substrate transporter upregulation, which preserves their density but is not sufficient to fully preserve their function. Intervention that supplies metabolic intermediates can mitigate the loss of MCT2. - Source: PubMed
Publication date: 2026/04/29
Murinda Kudakwashe PMorgan Autumn BInman Denise M - Mesenchymal stromal cells (MSCs) have been widely studied for their regenerative and immunomodulatory properties. However, clinical translation is hindered by a lack of, or limited, in vivo retention due to immune-mediated clearance. Biomaterial-based encapsulation, particularly using alginate hydrogels, offers a promising strategy to enhance MSC persistence and functionality. This study aimed to evaluate the impact of unmodified and RGD-functionalized GMP-grade alginate matrices on umbilical cord-derived MSCs (UC-MSCs) viability, mitochondrial function, and cytokine secretion profile. Cryopreserved UC-MSCs were encapsulated in GMP-compatible ultrapure alginates, an unmodified alginate (SLG20) and (G-RGD) modified with Arg-Gly-Asp (RGD) peptides, and compared to 2D cultures over five days for viability, metabolic activity, cytokine secretion, and mitochondrial function. MSCs encapsulation in G-RGD alginate significantly enhanced viability and modified cytoskeletal organisation compared to SLG20. While encapsulation resulted in 58% reduction in OXPHOS, relative to 2D culture at Day 0 ( < 0.01), with no significant difference between SLG20 and G-RGD. G-RGD-encapsulated cells maintained significantly higher basal, ATP-linked, and maximal respiration ( < 0.01) than SLG20-encapsulated cells for up to five days. Notably, encapsulation triggered a 60-fold upregulation of and a twofold increase in expression by Day 3, indicating metabolic adaptation and mitochondrial biogenesis signalling. Early cytokine profiling showed that encapsulation increased VEGF secretion by approximately 11-fold compared to 2D MSCs. IL-6 secretion was 34.5% higher in G-RGD than in SLG20 at Day 1 and was markedly higher in encapsulated MSCs than in 2D cultures. Although TNF-α secretion remained low overall, levels were 49% higher in G-RGD than in SLG20 at Day 5. IL-10 levels were similar between matrices. Encapsulation reduced glycolytic output by 67% compared to 2D cultures and lowered expression over time. This work was translation-focused, testing whether established RGD-related benefits are preserved under GMP-grade materials and can be clinically deployable with cryopreserved cells. Our findings reveal that a simple encapsulation in alginate microbeads, within 500 μm beads, creates a hypoxic environment for MSCs, similar to their natural niche, which strongly alters their functions as compared to the classical 2D plastic culture. G-RGD alginate provided modest but consistent advantages over unmodified SLG20 in maintaining mitochondrial function and modulating cytokine secretion. Matrix composition remains a critical factor in shaping MSC behaviour, and G-RGD ultrapure alginate represents a promising material for optimising cell-based therapies under clinically relevant conditions. - Source: PubMed
Publication date: 2026/05/13
Güven KadriyeDhawan AnilFilippi Celine - Understanding the chronic effects of intracortical microstimulation (ICMS) and device implantation on cortical function is essential for the development of stable neuroprosthetics. We chronically implanted penetrating microelectrodes into Thy1-GCaMP6s mice and conducted longitudinal mesoscopic widefield and two-photon Ca imaging alongside intrinsic optical signal recordings over 12 weeks. Six ICMS frequencies (10-500 Hz) and contralateral visual LED stimuli were delivered in repeated sessions. Oxygen extraction fraction (OEF) was estimated from dual-wavelength reflectance, and hemodynamic response functions (HRFs) were derived via regularized deconvolution. Over the chronic period, 25-Hz ICMS evoked progressively larger Ca responses with extending duration, while spatial spread remained stable after the first few days. Low-frequency ICMS preserved stable Ca depression, whereas high-frequency ICMS and visual stimulation showed partial declines in depression magnitude and spatial extent. Two-photon imaging revealed that somatic and neuropil compartments followed distinct activation and depression trajectories, with somatic ICMS responses declining then recovering while neuropil activation tracked mesoscale trends. Concurrently, OEF reductions deepened, reflecting increased relative blood supply, and the spatial extent of OEF signals contracted in the first week before expanding by day 21. Epileptiform Ca events emerged in nearly half of mice, predominantly under 25-Hz ICMS. HRF peak amplitude increased between day 0 and days 7-21, with latency decreasing. Spontaneous neural and hemodynamic activity near the probe was suppressed acutely but recovered over weeks. Chronic ICMS induces progressive potentiation of neuronal and vascular responses alongside local neurovascular uncoupling on day 0 and sustained silencing of spontaneous activity near the implant. These dynamics stabilized 6-7 weeks after implantation. The frequency-dependent epileptiform susceptibility, coupled with persistent focal neurovascular deficits, underscores the need for adaptive stimulation strategies during the pre-stabilization period and electrode designs that mitigate local suppression to ensure long-term stability of cortical neuroprostheses. - Source: PubMed
Publication date: 2026/05/02
Suematsu NaofumiVazquez Alberto LKozai Takashi Dy - Immunoglobulin A nephropathy (IgAN), the most common primary glomerulonephritis globally, leads to a high lifetime risk of kidney failure. Shenhua Tablet (SHT), a Chinese herbal formula, demonstrates clinical efficacy in IgAN. Paeoniflorin (PF), its principal active constituent, shows anti-inflammatory and renoprotective effects in preclinical models; however, its precise molecular mechanisms in IgAN remain unclear. - Source: PubMed
Publication date: 2026/05/01
Wang MengmengYin FengtingLi PingHan JinweiZheng YingYan GuangliLiu ChangWang YuhangChen XiangmeiYan XiaotongSun HuiGuan ShihanWang Xijun