P2X3, antiserum, guinea pig, 100 ul.
- Known as:
- P2X3, antibodies, guinea pig, 100 ul.
- Catalog number:
- GP10108-100
- Product Quantity:
- 1
- Category:
- -
- Supplier:
- Neuromi
- Gene target:
- P2X3 antiserum guinea pig 100 .
Related genes to: P2X3, antiserum, guinea pig, 100 ul.
- Gene:
- P2RX3 NIH gene
- Name:
- purinergic receptor P2X 3
- Previous symbol:
- -
- Synonyms:
- P2X3
- Chromosome:
- 11q12.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-10-09
- Date modifiied:
- 2016-10-05
Related products to: P2X3, antiserum, guinea pig, 100 ul.
Related articles to: P2X3, antiserum, guinea pig, 100 ul.
- The present study aims to elucidate the role of the Sigma‑1 receptor in the pathogenesis of neuropathic pain and evaluate its potential therapeutic implications. To systematically assess the effects of the Sigma‑1 receptor, neuropathic pain was induced in rats using the chronic constriction injury (CCI) model. Subjects were subsequently divided into three groups: Sham, CCI, and CCI+BD1047 (where BD1047 is a Sigma‑1 receptor antagonist). Following intrathecal administration of the respective agents, thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. Additionally, Western blotting was utilized to examine Sigma‑1 receptor, phosphorylated protein kinase Cα (p‑PKCα), and P2X3 receptor expression in the dorsal root ganglia (DRG). Immunofluorescence techniques were employed to examine p‑PKCα and P2X3 receptor expression. The results indicate a direct correlation between Sigma‑1 receptor activity and pain perception, evidenced by changes in TWL and MWT. In the CCI group, both TWL and MWT were significantly reduced compared to the Sham group. Furthermore, protein levels of the Sigma‑1 receptor, p‑PKCα, and P2X3 receptor in the DRG were elevated, and immunofluorescence expression of p‑PKCα and the P2X3 receptor also increased. Conversely, in the CCI+BD1047 group, TWL and MWT were significantly enhanced. Additionally, protein levels of the Sigma‑1 receptor, p‑PKCα, and P2X3 receptor in the DRG decreased, along with reduced immunofluorescence expression of p‑PKCα and P2X3 receptor. The findings indicate that neuropathic pain is intricately associated with the Sigma‑1 receptor, p‑PKCα, and P2X3 receptor in the dorsal root ganglia. Notably, the Sigma‑1 receptor regulates the expression of p‑PKCα and P2X3 receptor, presenting a novel therapeutic target for neuropathic pain management. - Source: PubMed
Publication date: 2025/10/20
Liu Shuang-ShuangYu XuanLi XueLi Qing-MeiMao Qing-XiangYuan JieQin Bang-Yong - P2X receptors, a family of ATP-activated ion channels, encompass subtypes P2X1-7, which are expressed in both homo- and heterotrimeric forms across various tissues. These receptors play crucial roles in pathophysiological processes such as synaptic transmission, nociception, cough, and taste perception. Extracellular ATP exists as both MgATP and ATP, with P2X3 responding to both. The evolutionary rationale for two nearly identical ligands and their distinct signaling potential remains unclear. While previous structural studies suggest a uniform ATP recognition mechanism for two endogenous ATP forms, we propose that MgATP and ATP activate P2X3 through distinct mechanisms, leading to differential physiological and pathological outcomes. Using mutagenesis, voltage-clamp fluorometry, and small molecule interventions, we identify divergent interactions of ATP and MgATP with P2X3, despite binding to the same orthosteric pocket. In transgenic mice, which selectively impair MgATP activation, we find that MgATP modulates ammonia-induced cough frequency without affecting complete Freund's adjuvant-induced inflammatory pain or sweet taste preference. mice show deficits in all three responses. The allosteric inhibitor aurintricarboxylic acid selectively modulates ATP and MgATP effects, resulting in distinct antitussive and analgesic outcomes in vivo. These findings uncover a mechanism of P2X3 activation by its endogenous ligands, diverging from previous structural models and resembling the biased activation mechanisms observed in G-protein-coupled receptors, offering insights for P2X3-targeted therapeutics. - Source: PubMed
Publication date: 2025/12/10
Wang JinGuan LiWang Ting-TingXie Tang-XuanLin Yi-YuSun Meng-YangWang Dong-PingZhang XueBian Yu-JingYue Chen-XiDing Bei-BeiMa Xue-FeiYu Ye - The bladder trigone is an anatomically and functionally distinct region within the lower urinary tract (LUT), characterized by a dense network of afferent sensory fibers, specialized urothelial interactions, and prominent mechanotransduction mechanisms. Its intricate neuroarchitecture enables precise detection of bladder filling and coordination of micturition, whereas dysregulation of these pathways contributes to lower urinary tract symptoms (LUTS), including urgency, frequency, and bladder pain. Despite its recognized clinical relevance, the structural and functional basis of trigonal sensory signaling - and its role - remain incompletely understood. This review synthesizes current evidence on trigonal afferent organization, integrating data from anatomical mapping, receptor profiling, electrophysiological characterization, and translational research. Seminal anatomical observations are combined with recent advances in mechanotransduction and purinergic, peptidergic, and transient receptor potential (TRP) signaling to provide a comprehensive perspective. The trigone exhibits three principal afferent classes: (1) intraepithelial fibers penetrating umbrella cells, marked by P2X purinoceptor 3 (P2X3), transient receptor potential vanilloid 1 (TRPV1), calcitonin gene-related peptide (CGRP), and substance P (SP); (2) subepithelial plexuses surrounding microvasculature, enriched in vasoactive neuropeptides and exhibiting plastic hypertrophy in overactive bladder (OAB) and interstitial cystitis/bladder pain syndrome (IC/BPS); and (3) encapsulated corpuscular endings at the lamina propria-detrusor junction, expressing PIEZO1/2 and acid-sensing ion channels (ASICs) for rapid adaptation. In trigeminal dorsal root ganglion (DRG) neurons, high expression of PIEZO2, P2RX3, and voltage-gated sodium channel, type 1.8 (Nav1.8) was observed, revealing their role as the foundation for multisensory information processing. Functional assays highlight distinct mechanotransductive and chemosensory pathways, with aging, inflammation, and neurotrophic factors driving afferent plasticity underlying abnormal bladder sensation, such as urgency, frequency, and pain. Early clinical trials of P2X3 antagonists and intravesical TRPV1 inhibitors demonstrate promising symptomatic benefits. Collectively, evidence positions the bladder trigone as a critical sensory hub where neuronal, urothelial, and immune signals converge to regulate bladder sensation. Understanding its molecular and structural specialization may inform the development of region-specific neuromodulatory therapies targeting sensory urgency and afferent-driven bladder dysfunction. - Source: PubMed
Publication date: 2025/10/19
Sadahira TakuyaMaruyama YukiMitsui YosukeSekito TakanoriWatanabe TomofumiWatanabe Masami - The hippocampus is a key component of the brain that is associated with the formation of longterm memory, the energy metabolism of neurons playing a pivotal role in its mechanisms. The P2X3 receptor in the hippocampus is considered an attractive target when searching for novel biologically active substances that could work to reduce anxiety, epileptic conditions, and improve cognitive functions. In this work, the intensity of mitochondrial respiration, the glycolytic capacity, and the energy phenotype of hippocampal neurons were studied in knockout mice. The knockout mice were engineered by genome editing using the CRISPR/Cas9 system. The primary mixed culture of hippocampal neurons was derived from two-day-old newborn mice with the and genotypes. Mitochondrial respiration was measured on a Seahorse Bioscience HS mini Cell Metabolism Analyzer (Agilent, USA) using the appropriate kits for the Mitostress test, glycotest, and energy phenotype assessment test. The transgenic mice with the genotype were characterized by an aerobic type of mitochondrial respiration, an increase in ATP production by 84.4% ( < 0.05), an increase in maximum respiration by 72.3% ( < 0.05), and a 36% ( < 0.05) increase in the respiratory reserve. Meanwhile, the spare respiratory capacity of mitochondria, the rate of glycolysis, and the glycolytic capacity in these mice were reduced by 36.6, 75.7 and 78.6% ( < 0.05), respectively. Our findings indicate that mitochondria work at close to maximum energy capacity. The knockout animals are a unique model for the search for pharmacological targets that can help correct the energy metabolism of brain cells and eliminate cognitive dysfunctions. - Source: PubMed
Zelentsova A SPokrovskii M VPatrakhanov E AShmigerova V SSkorkina M YuDeykin A V - This study investigated the effects of dietary resveratrol (RES) and β-Hydroxy β-Methylbutyrate (HMB) on immune function, oxidative status, and morphological changes in intermuscular fat of Tibetan sheep. Previous research suggests that RES and HMB may enhance muscle quality and lipid metabolism, but their combined effects on meat flavor, fatty acid composition, and underlying molecular mechanisms remain unclear. Therefore, we employed transcriptomics and lipid metabolomics to explore how RES and HMB synergistically regulate key signaling pathways and lipid metabolites to improve meat quality. - Source: PubMed
Publication date: 2025/09/03
Chen XuanJi QiurongWu ZhenlingZhang FengshuoSu QuyangangmaoHe TingliZhu KainaHou ShengzhenGui Linsheng