Nanog Antibody
- Known as:
- Nanog Antibody
- Catalog number:
- 3165-100
- Product Quantity:
- 100
- Category:
- -
- Supplier:
- Biovis
- Gene target:
- Nanog Antibody
Related genes to: Nanog Antibody
- Gene:
- NANOG NIH gene
- Name:
- Nanog homeobox
- Previous symbol:
- -
- Synonyms:
- FLJ12581, FLJ40451
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 2003-09-10
- Date modifiied:
- 2014-11-19
Related products to: Nanog Antibody
Related articles to: Nanog Antibody
- Medulloblastoma (MB), a malignant tumour arising in the brain, exhibits distinct microRNA expression patterns. One such microRNA, miR-30b-5p, has been relatively unexplored in its function within MB cells. This study investigates the regulatory role of miR-30b-5p in MB cells, particularly its influence on the Sonic Hedgehog signaling pathway through Gli1 mediation. Daoy MB cells were transfected with miR-30b-5p, a miR-negative control, or left Untreated. Cell proliferation assays, Reverse transcription PCR, and RT-qPCR were conducted to assess cell growth and target gene expression. The findings revealed that miR-30b-5p regulates DAOY cell growth at 48 and 72 h after transfection, but exhibits an inhibitory effect at 24 h compared to the miR-Negative Control group. Furthermore, the expression of genes GLI1, BMI1, P53, and NANOG was examined. At 24 h, these genes displayed lower expression in the miR-30b-5p group. Conversely, at 48 h, BMI1 and P53 expression continued to decrease, while GLI1 and NANOG showed increased expression compared to the miR-negative control group. In conclusion, the upregulation of miR-30b-5p resulted in decreased apoptosis and enhanced medulloblastoma proliferation. This effect was mediated through the positive regulation of Gli1 via the Sonic Hedgehog pathway, leading to increased expression of GLI1 and NANOG. These findings suggest a potential role for miR-30b-5p as a therapeutic target in MB treatment. - Source: PubMed
Publication date: 2024/10/29
Ahmed Swalih PShahi Mehdi H - To investigate the in vitro effects of electric field (EF) stimulation on the proliferation, migration, stemness, and differentiation potential of dental pulp stem cells (DPSCs), and to explore its potential relevance to dentin-pulp regeneration. - Source: PubMed
Publication date: 2026/07/23
Li XiaolinGu YuZhong QiulingLiu QianTelezhkin VsevolodSloan Alastair JQiu LihongSong Bing - X-linked retinoschisis (XLRS) is an inherited retinal degenerative disease caused by mutations in the RS1 gene, leading to visual impairment. The RS1 c.214G>A mutation is a clinically relevant variant associated with XLRS. In this study, we generated a human induced pluripotent stem cell (iPSC) line (CSUASOi016-A) carrying the RS1 c.214G>A (p.E72K) mutation using CRISPR/Cas9. The edited iPSC line exhibited typical pluripotent stem cell morphology, expressed pluripotency markers (OCT4, SSEA4, SOX2, and NANOG), and retained the ability to differentiate into all three germ layers. This cell line provides a valuable resource for modeling XLRS pathogenesis and developing therapeutic strategies. - Source: PubMed
Publication date: 2026/07/16
Duan ChunwenSun XihaoDing ChengchengMao ShengruLiang YuqinLiang YuanZhang RutingChen HangChen JiangsuTang Shibo - Proteins and nucleic acids form non-Newtonian liquids with complex rheological properties that contribute to their function in vivo. Here, we investigate the rheology of the transcription factor NANOG, a key protein to maintain embryonic stem cell pluripotency. We find that, at high concentrations, NANOG forms macroscopic aging gels that are dependent on its intrinsically disordered domain. By combining molecular dynamics simulations, mass photometry, and cryo-EM, we also discover that-in contrast to unbounded condensates formed by other intrinsically disordered proteins-NANOG forms self-limiting micelles with exposed DNA-binding domains. We show that these micelles can stabilize DNA entanglements and, in turn, modulate DNA dynamics. Based on our findings, we conjecture that NANOG may contribute to regulate gene expression by creating local gel-like environments that restrict genome dynamics and that its aging may ingrain mechanical memory in gene regulatory networks. - Source: PubMed
Hong-Minh AmandineFosado Yair Augusto GutiérrezGuild AbbieMullin NicholasSpagnolo LauraChambers IanMichieletto Davide - Although phosphorodiamidate morpholino oligomers (PMOs) are potent and stable antisense agents, their neutral backbone limits cellular uptake and biodistribution, requiring specialized delivery strategies to improve therapeutic efficacy. Chimeric guanidinium-linked morpholino oligomers (GMOs) with PMOs are designed to enhance cellular delivery yet compromise hybridization with the target sequence due to backbone rigidity. To address this, we designed flexible GMO-PMO chimeras by incorporating sarcosine, β-alanine, and ethanamine as internucleotide spacers in the oligonucleotide. Thermal melting studies on a 19-mer polythymidine (polyT) sequence demonstrate that these flexible GMO-PMOs partially restore duplex stability in a modification-dependent manner. This structural restoration is also observed in biologically relevant mixed GMO-PMO sequences targeting a gene linked to stemness and cancer biology, while retaining their B-type global geometry as confirmed by circular dichroism analysis. The optimized ethanamine-modified chimera elicited strong, dose-dependent silencing, with up to 95% repression in MCF7 and ∼73% in HCT116 cells at 1 μM after 48 h. Importantly, both rigid and flexible GMO-PMOs exhibit similar lysosomal sequestration, varying in a cell-line-dependent manner. Collectively, flexible GMO-PMO chimeras mitigate the long-standing trade-off between carrier-free PMO delivery and duplex stability, establishing a robust platform for antisense gene silencing and potential therapeutic applications. - Source: PubMed
Publication date: 2026/07/21
Qasim MohammedNaaz SharmeenPratihar SubhamoySharma Swrajit NathSinha Surajit