HRH3
- Known as:
- HRH3
- Catalog number:
- Y214408
- Product Quantity:
- 200ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- HRH3
Related genes to: HRH3
- Gene:
- HRH3 NIH gene
- Name:
- histamine receptor H3
- Previous symbol:
- -
- Synonyms:
- GPCR97
- Chromosome:
- 20q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 2000-01-07
- Date modifiied:
- 2015-08-25
Related products to: HRH3
Related articles to: HRH3
- Histamine receptors (HRH1-HRH4) are G-protein coupled receptors (GPCRs) that mediate essential physiological functions, including neurotransmission, gastric acid secretion, immune regulation, and presynaptic autoregulation. Despite their importance, systematic comparative analyses across mammalian histamine receptor sequences remain scarce. In this study, we performed a comprehensive evaluation of HRH1-HRH4 across multiple mammalian species, integrating sequence homology, invariant residue mapping, amino acid substitutions, compositional frequencies, Shannon entropy, polystring distributions, intrinsic disorder profiling, and phylogenetic clustering. HRH2 and HRH1 were highly conserved among primates, HRH3 showed strong cohesion within rodents, while HRH4 exhibited pronounced divergence consistent with immune-related specialization. Invariant residues localized to transmembrane helices and activation motifs (D107, W428/Y431, NPxxY), underscoring strict evolutionary constraints on ligand binding, receptor stability, and G-protein coupling. Substitutions were confined to non-essential lipid-facing and loop regions, predominantly conservative in nature, enabling diversification without disrupting the GPCR fold. Amino acid frequency and entropy analyses revealed hydrophobic dominance with subtype-specific enrichment of polar residues, while disorder profiling identified HRH1 as the most dynamic and HRH2 as the most structurally constrained. Polystring analysis highlighted conserved motifs (WWW, PP) alongside subtype- and species-specific repeats, reflecting evolutionary strategies balancing receptor stability with adaptive flexibility. Phylogenetic clustering confirmed subtype-specific cohesion, with HRH3 and HRH4 forming compact clades, HRH1 showing moderate dispersion, and HRH2 forming the most isolated cluster. The central focus of this study lies in the characterization of both structural features and evolutionary trajectories of histamine receptors, providing a unified perspective on their conservation and diversification. Collectively, these findings demonstrate that mammalian histamine receptor evolution is governed by conserved biophysical cores and selective variability, offering insights into structural conservation, functional diversification, and translational relevance for drug design and model selection. - Source: PubMed
Publication date: 2026/04/04
Hassan Sk SarifNawn DebaleenaGoswami PritamMukherjee NabanitaSil MoumitaRoy SujanGoswami ArunavaDas SatadalUversky Vladimir N - Psychoactive substance use (PSU) and cancer are frequently observed comorbidities that have reciprocal influences and shared behavioral traits of the affected patients. While, e.g., nicotine and alcohol are major carcinogens in the etiology of lung and head and neck cancers, little is known about a shared overarching genetic architecture of PSU and cancer that may predispose individuals to both illnesses. - Source: PubMed
Publication date: 2026/02/05
Song JiahangLi PengzhuCanis MartinUnger KristianHaas Nikolaus AlexanderGires Olivier - Rett syndrome (RTT) and Duplication syndrome (MDS) are disorders caused by reciprocal decreases and increases in the expression of the transcriptional regulator, (). We previously performed an mRNA expression profiling study of the temporal cortex region from patients diagnosed with RTT and corresponding age, postmortem interval, and sex-matched controls. These studies identified a significant reduction in the expression of the histamine H receptor (). In the current manuscript, we expanded this H receptor profiling to additional RTT patient brain samples representing distinct mutations and confirmed significantly reduced levels of H receptor expression in the majority of patients compared to controls. Using mouse models of RTT and MDS, we observed antiparallel changes in H receptor expression across various brain areas, with expression being reduced in RTT model animals and increased in a mouse model of MDS. We then evaluated both a small molecule agonist of the H receptor, ()-α-methylhistamine (RAMH), and the H receptor inverse agonist, pitolisant (Wakix), in RTT and MDS models, respectively, to determine impacts on phenotypes in these disease models. Our results show that RAMH significantly impacted an anxiety phenotype in mice modeling RTT ( ), but pitolisant had no effect on the behaviors examined here in MDS animals ( ). - Source: PubMed
Publication date: 2025/12/20
Weiss KellyVermudez Sheryl A DFreitas GeanneDogra ShaliniMeadows Mac JGogliotti Rocco GNiswender Colleen M - (1) Glioblastoma (GBM) is one of the most aggressive brain tumors with a poor prognosis. Therefore, new insights into GBM diagnosis and treatment are required. In addition to differentially expressed mRNAs, miRNAs may have the potential to be applied as diagnostic biomarkers. (2) In this study, profiling of human miRNAs in combination with mRNAs was performed on total RNA isolated from tissue samples of five control and five GBM patients, using a high-throughput RNA sequencing (RNA-Seq) approach. (3) A total of 35 miRNAs and 365 mRNAs were upregulated, while 82 miRNAs and 1225 mRNAs showed significant downregulation between tissue samples of GBM patients compared to the control samples using the iDEP to analyze RNA-Seq data. To validate our results, the expression of five miRNAs (hsa-miR-196a-5p, hsa-miR-21-3p, hsa-miR-10b-3p, hsa-miR-383-5p, and hsa-miR-490-3p) and fourteen mRNAs (E2F2, HOXD13, VEGFA, CDC45, AURKB, HOXC10, MYBL2, FABP6, PRLHR, NEUROD6, CBLN1, HRH3, HCN1, and RELN) was determined by RT-qPCR assay. The miRNet tool was used to build miRNA-target interaction. Furthermore, a protein-protein interaction (PPI) network was created from the miRNA targets by applying the NetworkAnalyst 3.0 tool. Based on the PPI network, a functional enrichment analysis of the target proteins was also carried out. (4) We identified an miRNA panel and several deregulated mRNAs that could play an important role in tumor development and distinguish GBM patients from healthy controls with high sensitivity and specificity using total RNA isolated from tissue samples. - Source: PubMed
Publication date: 2025/03/19
Géczi DóraKlekner ÁlmosBalogh IstvánPenyige AndrásSzilágyi MelindaVirga JózsefBakó AndreaNagy BálintTorner BernadettBirkó Zsuzsanna - This study investigates the expression and clinical significance of the histamine receptor family (HRs) in the bone marrow of children with newly diagnosed acute myeloid leukemia (AML). - Source: PubMed
Publication date: 2025/02/07
Zhou BiWang WenPengYu PaoYang YangMi DaWeiTian YuanYuanLi YingZhu Feng