FBXO32
- Known as:
- FBXO32
- Catalog number:
- Y214141
- Product Quantity:
- 200ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- FBXO32
Related genes to: FBXO32
- Gene:
- FBXO32 NIH gene
- Name:
- F-box protein 32
- Previous symbol:
- -
- Synonyms:
- MAFbx, ATROGIN1, Fbx32
- Chromosome:
- 8q24.13
- Locus Type:
- gene with protein product
- Date approved:
- 2001-10-04
- Date modifiied:
- 2014-11-18
Related products to: FBXO32
Antibodies: FBXO32 HOST: Goat Clonality: pAbAtrogin-1,F-box only protein 32,FBXO32,Homo sapiens,Human,MAFbx,Muscle atrophy F-box proteinAtrogin-1,F-box only protein 32,Fbxo32,MAFbx,Mouse,Mus musculus,Muscle atrophy F-box proteinAtrogin-1,F-box only protein 32,Fbxo32,MAFbx,Muscle atrophy F-box protein,Rat,Rattus norvegicusBos taurus,Bovine,F-box only protein 32,FBXO32Bovine F-box only protein 32(FBXO32) ELISA kitBovine F-box only protein 32(FBXO32) ELISA kitBovine F-box only protein 32(FBXO32) ELISA kit SpeciesBovineBovine F-box only protein 32,FBXO32-MAFbx ELISA KitBovine F-box only protein 32,FBXO32-MAFbx ELISA KitBovine F-box only protein 32,FBXO32/MAFbx ELISA KitBovine F-box only protein 32,FBXO32/MAFbx ELISA KitBovine F-box only protein 32,FBXO32/MAFbx ELISA KitBovine F-box protein 32 (FBXO32) ELISA kit, Species Bovine, Sample Type serum, plasmaCanine F-box only protein 32(FBXO32) ELISA kit Related articles to: FBXO32
- F-box proteins serve as substrate adapters in SCF ubiquitin ligase complexes, regulating proteolysis to modulate eukaryotic signaling, such as cell cycle progression and stress responses. Here, we characterize the F-box gene family in water buffalo (Bubalus bubalis), identifying 70 genes across 24 autosomes and the X chromosome using HMMER and BLASTP searches against Pfam domains. Phylogenetic analysis with orthologs from human, sheep, goat, horse, and cattle grouped them into four subfamilies (FBX, FBXL, FBXW, FBXO), with 58 core buffalo genes aligning closely to mammalian clades (bootstrap >95%). MEME analysis identified nine conserved motifs, including the F-box domain (motifs 1-2, e.g., LPDELLLYIFSYLDA), LRRs for specificity (motif 3), and WD40 repeats for scaffolding (motifs 5-7,9), confirmed by Pfam. Gene structures ranged from 2 to 23 exons, indicating regulatory diversity. Synteny with cattle conserved ∼85% of orthologs, reflecting artiodactyl ancestry. Physicochemical properties spanned MW 18.10-93.71 kDa (mean 49.2 kDa, excluding outliers), pI 4.27-10.41, and GRAVY -0.75 to 0.143 (mean - 0.32), consistent with hydrophilic profiles. Five segmental duplications (e.g., FBXO25/FBXO32) showed Ka/Ks ratios of 0.144-1.194 (four <1), suggesting purifying selection and divergence 48-220 MYA (Ks Poisson correction, refined ruminant rate). nsSNP analysis in 70 genes via nine tools predicted >95% benign/synonymous effects, with deleterious exceptions like FBXO16 V160T (consensus score 0.82, disrupting binding) and FBXO48 R7G (0.91, affecting stability). WoLF PSORT localized 17 nuclear, 10 cytoplasmic, 12 plasma membrane, 7 mitochondrial, 5 extracellular, and 7 dual sites, implying roles in transcription, degradation, and trafficking. These genes may represent potential candidates for future functional studies and genome-editing approaches; however, experimental validation will be required before their application in breeding programs. - Source: PubMed
Publication date: 2026/04/04
Nasir TanveerSafdar MuhammadImran SafdarTariq MuhammadJunejo YasmeenOzaslan MehmetYounus Muhammad - - Source: PubMed
Publication date: 2026/04/10
Pu TianFeng RanranWang ChunruZhao Ye - Mature cow weight (MWT), height (MHT), and body condition score (BCS) are economically important traits that significantly influence cowherd profitability by affecting maintenance feed requirements, which is the largest component of production costs. This study aimed to identify genomic regions, candidate genes, and pleiotropic variants associated with mature cow size traits in American Angus cattle, and to characterize their related gene ontology terms and metabolic pathways. The dataset provided by the American Angus Association comprised 434,746 MWT records from 222,907 animals; 213,875 MHT records from 112,987 animals; and 382,156 BCS records from 209,696 animals. Of these, 45,606 cows were genotyped and imputed to a common marker density of 54,609 markers. A final dataset of 51,410 SNPs from 45,452 animals remained after quality control for further analyses. The single-step genome-wide association study (ssGWAS) method was used in the analyses. Significant associations for mature cow size were detected on BTA7, BTA14, and BTA20, overlapping with previously reported QTLs for growth, feed efficiency, and carcass traits. Candidate genes such as , , , , , and were found to be related to muscle development, skeletal growth, lipid metabolism, and energy regulation. The genes , , , , , , , , , , and emerged as candidate genes exhibiting pleiotropic effects on mature cow size traits. Pathway enrichment highlighted the roles of insulin/IGF1R signaling, fibroblast growth factor receptors cascades, and mitogen-activated protein kinase pathways in MWT and MHT, while for BCS, only RHOD GTPase cycle was enriched. Pleiotropy-based analysis of regions affecting mature cow size traits identified shared genomic loci, and subsequent pathway analysis revealed G protein signaling as a common regulatory mechanism linking energy balance, adiposity, and growth. These results contribute to a better understanding of the genomic regions associated with mature cow size traits in Angus cattle. - Source: PubMed
Publication date: 2026/04/06
Ojo Ayooluwa OMulim Henrique ACampos Milena A FGarcia AndréRetallick-Riley KelliFonseca Pablo A SOliveira Hinayah R - Epithelial-myoepithelial carcinoma (EMC) is a rare malignant tumor of the salivary glands, often characterized by HRAS mutations. The present study aimed to better define the molecular and transcriptomic landscape of EMC and explore its relationship with other head and neck neoplasms, particularly basal cell adenoma (BCA). We retrospectively analyzed 14 EMC cases using histology, immunohistochemistry, and whole-exome capture RNA sequencing. HRAS mutations were identified in 57.0% (8/14) of EMC, predominantly at p.Gly61Arg. Additional mutations identified included PIK3CA, STAT5B, and NOTCH1, as well as gene fusions such as HMGA2::WIF1 and FBXO32::PLAG1. A comparative analysis with 54 RAS-mutated head and neck tumors revealed that HRAS mutations were not exclusive to EMC, as they were also found in benign entities such as BCAs. Transcriptomic profiling found that EMC and BCA shared significant gene expression similarities, clustering closely. Differential expression analysis found upregulation of GATA3, CHI3L1, GRIA2, and MME in EMC, while BCAs had enrichment of Wnt-beta-catenin signaling. In contrast, EMCs had activation of the Ras-Raf-MAPK and PI3K-Akt pathways, supported by upregulation of E2F and G2-M checkpoint targets. Immunohistochemistry confirmed GATA3 positivity in HRAS-mutated EMCs. The frequent RAS mutations and overlapping transcriptomic profiles raise important diagnostic considerations and highlight the need for integrated molecular analyses to differentiate these entities. The EMCs displayed a recurrent HRAS mutation, also observed in other head and neck tumors, and were partially clustered with the cases of BCA, suggesting potential biological similarities. - Source: PubMed
Publication date: 2026/03/25
Alsugair ZiyadDescotes FrançoiseChampagnac AnneLopez JonathanFieux MaximePhilouse PierreCéruse PhilippeThamphya BricePissaloux DanielTirode FranckBenzerdjeb Nazim - Cancer cachexia is a complex syndrome associated with chronic inflammation, catabolic dysregulation, and sympathetic nervous system hyperactivity contribute to energy imbalance and skeletal muscle and adipose tissue wasting. This study investigates the therapeutic potential of pindolol in a murine model of cancer cachexia. In vitro study evaluated the cachexia-inducing potential of B16F10 melanoma cells and the therapeutic effects of pindolol on 3T3-L1 adipocytes. In vivo, cachexia was induced in male C57BL/6 mice via subcutaneous B16F10 melanoma cell injection, followed by pindolol treatment until the tumor volume reached 5000 mm³. Body weight, food intake, inflammatory markers, muscle and adipose tissue mass, adipokine levels, metabolic markers, organ weights, and gene expression of E3 ubiquitin-protein ligase TRIM63, Muscle Atrophy F-box gene (atrogin-1), and hormone sensitive lipase (HSL) were assessed. B16F10-conditioned media along with isoproterenol induced lipolysis in 3T3-L1 adipocytes, while pindolol reversed this effect. In vivo, pindolol significantly improved body weight, food intake, and systemic inflammation while restoring adipokine levels. Pindolol also preserved skeletal muscle and adipose depots, improved glucose levels, enhanced insulin sensitivity, and lowered triglycerides and cholesterol. Histological analysis confirmed muscle and adipose tissue preservation. Pindolol downregulated TRIM63 and Atrogin-1, reducing muscle atrophy, and decreased HSL, suggesting reduced lipolysis evident from mRNA expression. Pindolol alleviates cancer cachexia by reducing inflammation, preserving muscle and adipose mass, and improving metabolic parameters, highlighting its potential as a therapeutic candidate. - Source: PubMed
Joshi MitPatel Bhoomika M