CHA _ TCFL5
- Known as:
- CHA _ TCFL5
- Catalog number:
- Y213596
- Product Quantity:
- 200ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- CHA _ TCFL5
Related genes to: CHA _ TCFL5
- Gene:
- TCFL5 NIH gene
- Name:
- transcription factor like 5
- Previous symbol:
- -
- Synonyms:
- Figlb, E2BP-1, CHA, bHLHe82
- Chromosome:
- 20q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 1998-11-19
- Date modifiied:
- 2016-04-06
Related products to: CHA _ TCFL5
390 MMP FRET Substrate IVMca - P - Cha - G - Nva - HA - Dpa - NH2390 MMP FRET Substrate IVMca - P - Cha - G - Nva - HA - Dpa - NH2anti-TCFL5anti-TCFL5anti-TCFL5 (1F2)anti-TCFL5 (1F2)anti-TCFL5 type: Primary antibodies host: MouseAntibodies: CHA _ TCFL5 HOST: Goat Clonality: pAbATP-specific succinyl-CoA synthetase subunit beta,Canis familiaris,Canis lupus familiaris,Dog,SCS-betaA,Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial,Succinyl-CoA synthetase beta-A chaBoc-Arg(Mts)-OH-CHA, peptide reagentsBoc-Arg(Mts)-OH-CHA, peptide reagentsCalcium-activated non-selective cation channel 1,Long transient receptor potential channel 4,LTrpC4,Ltrpc4,LTrpC-4,Melastatin-like 2,MLS2s,Rat,Rattus norvegicus,Transient receptor potential cation chaCHA TCFL5 Immunizing PeptideCHA antibodyCHA antibody Polyclonal Antibodies Primary antibodies Related articles to: CHA _ TCFL5
- Human saliva contains numerous factors, including DNA, RNA, and protein, that may reflect the health status of the individual. Many of these factors are contained within extracellular vesicles (EVs). The contents of EVs are thought to mirror the cytoplasm of the cell of origin, providing insight into the health of the cell. We investigated the RNA content from EVs isolated from saliva (salEVs) to determine if we could detect transcripts associated with neurodegenerative conditions. - Source: PubMed
Publication date: 2026/01/12
Wen SichengYu ChangKelsey Maxfield M GPereira MandyAlaimo HannahTeixeira EmPracht JennaDaiello Lori ADrake JonathanSedivy John MWu ZhijinQuesenberry PeterKreiling Jill A - Oligoasthenoteratozoospermia (OAT) is a prevalent situation of male infertility partly caused by genetic defects with largely undiscovered. To further unravel the genetic etiology of OAT, we recruited cases for whole-exome sequencing (WES) to screen candidate pathogenic mutations. Here, we identified a heterozygous missense mutation in transcription factor-like 5 (TCFL5) (NM_006602.4: c.1207G > A: p.E403K) from two infertile brothers born into a non-consanguineous family. TCFL5 was previously linked to male infertility since Tcfl5 male mice manifested infertile due to OAT, while Tcfl5 mice could not be generated. Sperm morphological analysis of these brothers exhibited a similar OAT phenotype to Tcfl5 mice. In vitro functional analysis performed to explore the pathogenicity of TCFL5 mutation. Regardless of no significant effect on the expression of mutant TCFL5 detected by western blotting and immunofluorescence, dual-luciferase reporter assay revealed a serious impact on its transcriptional regulatory function. Many crucial genes involved in spermatogenesis, such as DMRT1, DAZL, SYCE1, SPACA1, CNTROB, IFT88, HOOK1 and SPATA6, occurred transcriptional abnormalities after TCFL5 mutated. Our results showed that TCFL5 mutation disrupted the normal transcription of spermatogenesis genes, finally resulting in male infertility raised by OAT. Our work firstly linked TCFL5 mutation to male infertility in human, which provides a new perspective on the genetic underpinnings of OAT and a theoretical basis for clinic genetic counseling and treatment strategies selection. - Source: PubMed
Publication date: 2025/07/25
Yu KexinZhang WenchenWang YuXiang MingfeiZheng NaZhang JingjingZha XiaominDuan ZongliuWang FengsongCao YunxiaZhu Fuxi - Accurate specification of female and male germ cells during embryonic development is critical for sexual reproduction. Primordial germ cells (PGCs) are the bipotential precursors of mature gametes that commit to an oogenic or spermatogenic fate in response to sex-determining cues from the fetal gonad. The critical processes required for PGCs to integrate and respond to signals from the somatic environment in gonads are not well understood. In this study, we developed the first single-nucleus multiomics map of chromatin accessibility and gene expression during murine PGC development in both XX and XY embryos. Profiling of cell-type-specific transcriptomes and regions of open chromatin from the same cell captured the molecular signatures and gene networks underlying PGC sex determination. Joint RNA and ATAC data for single PGCs resolved previously unreported PGC subpopulations and cataloged a multimodal reference atlas of differentiating PGC clusters. We discovered that regulatory element accessibility precedes gene expression during PGC development, suggesting that changes in chromatin accessibility may prime PGC lineage commitment prior to differentiation. Similarly, we found that sexual dimorphism in chromatin accessibility and gene expression increased temporally in PGCs. Combining single-nucleus sequencing data, we computationally mapped the cohort of transcription factors that regulate the expression of sexually dimorphic genes in PGCs. For example, the gene regulatory networks of XX PGCs are enriched for the transcription factors, TFAP2c, TCFL5, GATA2, MGA, NR6A1, TBX4, and ZFX. Sex-specific enrichment of the forkhead-box and POU6 families of transcription factors was also observed in XY PGCs. Finally, we determined the temporal expression patterns of WNT, BMP, and RA signaling during PGC sex determination, and our discovery analyses identified potentially new cell communication pathways between supporting cells and PGCs. Our results illustrate the diversity of factors involved in programming PGCs toward a sex-specific fate. - Source: PubMed
Publication date: 2025/03/10
Alexander Adriana KRodriguez Karina FChen Yu-YingAmato CiroEstermann Martin ANicol BarbaraXu XinYao Humphrey H C - Accurate specification of female and male germ cells during embryonic development is critical for sexual reproduction. Primordial germ cells (PGCs) are the bipotential precursors of mature gametes that commit to an oogenic or spermatogenic fate in response to sex-determining cues from the fetal gonad. The critical processes required for PGCs to integrate and respond to signals from the somatic environment in gonads are not understood. In this study, we developed the first single-nucleus multiomics map of chromatin accessibility and gene expression during murine PGC development in both XX and XY embryos. Profiling of cell-type specific transcriptomes and regions of open chromatin from the same cell captured the molecular signatures and gene networks underlying PGC sex determination. Joint RNA and ATAC data for single PGCs resolved previously unreported PGC subpopulations and cataloged a multimodal reference atlas of differentiating PGC clusters. We discovered that regulatory element accessibility precedes gene expression during PGC development, suggesting that changes in chromatin accessibility may prime PGC lineage commitment prior to differentiation. Similarly, we found that sexual dimorphism in chromatin accessibility and gene expression increased temporally in PGCs. Combining single-nucleus sequencing data, we computationally mapped the cohort of transcription factors that regulate the expression of sexually dimorphic genes in PGCs. For example, the gene regulatory networks of XX PGCs are enriched for the transcription factors, TFAP2c, TCFL5, GATA2, MGA, NR6A1, TBX4, and ZFX. Sex-specific enrichment of the forkhead-box and POU6 families of transcription factors was also observed in XY PGCs. Finally, we determined the temporal expression patterns of WNT, BMP, and RA signaling during PGC sex determination, and our discovery analyses identified potentially new cell communication pathways between supporting cells and PGCs. Our results illustrate the diversity of factors involved in programming PGCs towards a sex-specific fate. - Source: PubMed
Publication date: 2024/09/25
Alexander Adriana KRodriguez Karina FChen Yu-YingAmato Ciro MEstermann Martin ANicol BarbaraXu XinHung-Chang Yao Humphrey - The incidence of esophageal cancer continues to increase worldwide. Current therapeutic approaches have limited efficacy, so in order to search for better markers of the disease, it is necessary to further elucidate its molecular pathogenesis. Regulation of gene expression by long non-coding Rnas plays a role in many diseases, however the role in esophageal cancer is unclear. The aim of this study was to elucidate the role and regulatory mechanism of long non-coding RNA NRSN2-AS1 in the progression of esophageal cancer. By real-time quantitative PCR, immunohistochemistry, RNA interference, western blotting, and double luciferase reporter gene analysis, we found that NRSN2-AS1 was up-regulated in esophageal cancer tissues and cell lines, and was closely related to disease stage and prognosis. Functional studies have shown that the silencing of NRSN2-AS1 inhibits the proliferation of esophageal cancer cells, induces apoptosis, and prevents cell migration and invasion. In mouse models, NRSN2-AS1 also promoted tumor growth. The transcription factor TCFL5 upregulates the transcription of NRSN2-AS1, which acts as a sponge for microRNA(miR)-874-5p, thereby upregulating the expression of the oncogene RELT. Activation of the NRSN2-AS1/miR-874-5p/RELT regulatory axis was validated in vivo. - Source: PubMed
Publication date: 2024/07/10
Yao WenjianLiu JianHou ZhaoyaoJia XiangboYang DongFeng MingyuWu SenWei Li