Dph2
- Known as:
- Dph2
- Catalog number:
- 048187A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- Dph2
Related genes to: Dph2
- Gene:
- DPH2 NIH gene
- Name:
- diphthamide biosynthesis 2
- Previous symbol:
- DPH2L2
- Synonyms:
- -
- Chromosome:
- 1p34.1
- Locus Type:
- gene with protein product
- Date approved:
- 1998-03-24
- Date modifiied:
- 2019-02-14
Related products to: Dph2
Related articles to: Dph2
- Estrogen exerts a multifaceted influence on breast cancer, particularly through its association with estrogen receptor (ER) and progesterone receptor (PR), which serve as pivotal prognostic and therapeutic markers. While the differential expression of metabolic genes and their prognostic relevance in breast cancer have been extensively studied, limited research has examined their regulation by estrogen signaling. This study adopts a novel approach by investigating the effect of estrogen signaling on the expression of a broad spectrum of metabolic genes in breast cancer. - Source: PubMed
Publication date: 2025/12/01
Mazumder ArchismanSuryansh SuryanshSahoo Om SaswatSingh PrithviGoel IshaTalukdar JoyeetaSrivastava TryambakRanjan PiyushRai AvdheshDhar RubyKarmakar Subhradip - A comprehensive density functional theory investigation was conducted to elucidate the regio- and stereoselectivity of the 1,3-dipolar cycloaddition reaction (13-DCR) between a diazo compound (1,3-dipole D-1) and an electron-deficient nitroethylene derivative (dipolarophile Dph-2), which serves as the key initiating step in a recently reported domino synthesis of pyrazole derivatives. Experimentally performed in dichloromethane at 80 °C, the reaction exhibits exclusive formation of the regioisomer. Calculated activation free energies and rate constants quantitatively reproduce the experimentally observed complete regioselectivity and high stereoselectivity. Activation strain model (ASM) analysis reveals that the regioselectivity is primarily governed by stabilizing interaction energies. Subsequent energy decomposition analysis using the recently developed sobEDA method identifies orbital interactions, particularly HOMOD-1 to LUMODph-2 charge transfer, as the main contributors. The Extended Transition State-Natural Orbitals for Chemical Valence (ETS-NOCV) analysis confirms this dominant orbital interaction, rationalizing the selectivity. The stereoselectivity favoring the pathway is attributed to lower total strain energy, mainly originating from the flexibility of the D-1 fragment. This combined computational study offers a detailed mechanistic understanding of the 13-DCR regio- and stereoselectivity, providing results in excellent agreement with experimental outcomes. - Source: PubMed
Publication date: 2025/12/03
Emamian SaeedrezaShariati Ali MohammadSalami MajidFrontera Antonio - Neurodevelopmental disorders (NDDs) represent a clinically diverse group of conditions that affect brain development, often leading to varying degrees of functional impairment. Many NDDs, particularly syndromic forms, are caused by genetic mutations affecting critical cellular pathways. Ribosomopathies, a subgroup of NDDs, are linked to defects in ribosomal function, including those involving the synthesis of diphthamide, a post-translational modification of translation elongation factor 2 (eEF2). Loss-of-function (LoF) mutations in genes involved in diphthamide biosynthesis, such as , , and , result in developmental delay (DD), intellectual disability (ID), and multisystemic abnormalities. DPH5-related diphthamide deficiency syndrome has recently been reported as an ultrarare disorder linked to LoF mutations in , encoding a methyltransferase required for diphthamide synthesis. - Source: PubMed
Publication date: 2025/07/02
Politano DavideMancini CeciliaCelario MassimilianoRadio Francesca ClementinaD'Abrusco FulvioGarau JessicaKalantari SilviaVisani GaiaCarbonera SimoneGana SimoneFerilli MarcoChiriatti LuigiCappelletti CamillaEllena KatiaProdi ElenaBorgatti RenatoValente Enza MariaOrcesi SimonaTartaglia MarcoSirchia Fabio - -Adenosylmethionine (SAM) serves as an important substrate in a variety of biochemical reactions, and it is important to identify unknown SAM-binding proteins to fully understand the biological functions of SAM. Previous studies on SAM-binding proteins used -Adenosylhomocystein (SAH)-analogues, which mainly identified SAM dependent methyltransferases. Here, we developed and validated three SAM photoaffinity probes to label and enrich SAM-binding proteins. These probes efficiently labeled the known SAM-binding protein Dph2 involved in diphthamide biosynthesis from cell lysates. Using these probes, we enriched SAM-binding proteins from the cell lysates of and . In addition, we validated five SAM binders and revealed the SAM cleavage activities of three of them, including the radical SAM enzyme ArsL, which cleaves SAM to generate methylthioadenosine (MTA), and AcnA and EDD84_07545, which generate -adenosyl-l-homocysteine (SAH). Therefore, our SAM-based photoaffinity probes are promising tools for the identification of SAM-binding proteins. - Source: PubMed
Publication date: 2025/06/03
Wu XiangyuDong Min - -Adenosylmethionine (SAM) frequently functions as a cofactor or precursor for enzymes, initiating an array of radical reactions in biological systems. In contrast with the conventional 5'-deoxyadenosyl (dAdo) radical pathway, which proceeds via homolytic cleavage of the S-C(5') bond of SAM, the Dph2 enzyme provides an alternative 3-amino-3-carboxypropyl (ACP) radical pathway through breaking the S-C(γ) bond. Inspired by this distinctive bond cleavage mode, we have developed a chemically induced pathway to generate an ACP-type radical intermediate on methionine-based sulfonium. This strategy presents a novel desulfurization conjugation mode for methionine modification, diverging from previous approaches that conjugate onto the sulfur atom or the adjacent methyl group of methionine. The versatility of this strategy is demonstrated by the efficient functionalization of various peptides and peptide macrocyclizations. Density Functional Theory (DFT) calculations provide further insights into the mechanism of this desulfurization reaction, explaining the exceptional selectivity of homolytic cleavage of the S-C(γ) bond of methionine-based sulfonium. The successful implementation of this novel desulfurization strategy represents a substantial advancement in the understanding of sulfonium-based intramolecular radical substitution reactions and provides new opportunities for the functionalization of biomolecules, thereby fostering progress in interdisciplinary research. - Source: PubMed
Publication date: 2025/05/05
Zhang YueYu HuixinTang FengZhang Feng-HuaZhang MeihuiDong JinhuaZhao JianweiHuang WeiLiu Bo