Cstf3
- Known as:
- Cstf3
- Catalog number:
- 046106A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- Cstf3
Related genes to: Cstf3
- Gene:
- CSTF3 NIH gene
- Name:
- cleavage stimulation factor subunit 3
- Previous symbol:
- -
- Synonyms:
- CstF-77
- Chromosome:
- 11p13
- Locus Type:
- gene with protein product
- Date approved:
- 1995-01-16
- Date modifiied:
- 2016-04-05
Related products to: Cstf3
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- Inflammatory myofibroblastic tumor (IMT) of the breast is an uncommon neoplasm that presents significant diagnostic challenges due to its overlap with more common breast lesions. Clinical and imaging features are often nonspecific, leading to potential misdiagnosis. We present a breast IMT in a 38-year-old woman, initially detected as a 7-mm lesion classified as BI-RADS 3 during routine screening and suspected to represent fibrocystic changes. Follow-up ultrasound demonstrated growth to 10 mm, prompting a core needle biopsy that revealed a spindle cell lesion with prominent inflammatory infiltrates. Immunohistochemistry showed positivity for ALK, CD34, and NTRK, and fluorescence in situ hybridization (FISH) confirmed an rearrangement. Targeted RNA sequencing identified a novel fusion. This report highlights the diagnostic complexity of IMT and underscores the importance of comprehensive histological and molecular evaluation. Accurate diagnosis is essential for appropriate management and adds to the limited body of literature on breast IMT, particularly regarding the significance of genetic testing in confirming the diagnosis. - Source: PubMed
Publication date: 2026/05/10
Garlin Politis MichelleYilmaz MineChen DianeWiechmann LisaHibshoosh HaninaDerakhshan FatemehSalem Alireza - The laminin-binding integrin α3β1 is highly expressed in epidermal keratinocytes, where it coordinates diverse cellular functions and gene expression during skin remodeling. Here, we show that α3β1-MEK/ERK signaling operates in vivo to promote proximal polyadenylation site (PAS) usage in the Mmp9 gene, generating a short, more stable mRNA transcript. Using mice with inducible, epidermis-specific α3 deletion, RNA in situ hybridization revealed that loss of α3β1 increased the long Mmp9 transcript in healing wounds and epidermal tumors. α3β1-MEK/ERK signaling in keratinocytes induced the expression of the cleavage stimulation factor CSTF3, a known regulator of alternative polyadenylation (APA), while CSTF3 knockdown shifted Mmp9 toward distal PAS usage. Moreover, α3 deletion reduced Cstf3 gene expression and altered APA in vivo. Genome-wide DaPars2 analysis identified α3β1-dependent APA across numerous genes, including some encoding components of the keratinocyte secretome. Together, these findings define a novel α3β1-MEK/ERK-CSTF3 axis that orchestrates post-transcriptional gene regulation through APA, revealing α3β1 as a potential target for wound and cancer therapies. - Source: PubMed
Publication date: 2026/02/10
Albeche Duarte GiesseBossardi Ramos RamonWu LeiLongmate Whitney MDiPersio C Michael - RNA modifications are associated to various human diseases. However, the functions of RNA modification-related genes have yet to be thoroughly investigated in dilated cardiomyopathy (DCM). This study sought to conduct a comprehensive analysis of RNA modification-associated genes for the diagnosis and subtype classification of DCM. - Source: PubMed
Publication date: 2025/05/15
Xu CuixiangZhao XiangrongLi HuitingLi YapingFeng YangmengZhang GuoanHuang Xiaoyan - Platinum-based chemotherapy is the standard postoperative adjuvant treatment for ovarian cancer (OC). Despite the initial response to chemotherapy, 85% of advanced OC patients will have recurrent disease. Relapsed disease and platinum resistance are the major causes of death in OC patients. In this study, we compared the global regulation of alternative polyadenylation (APA) in platinum-resistant and platinum-sensitive tissues of OC patients by analyzing a set of single-cell RNA sequencing (scRNA-seq) data from public databases and found that platinum-resistant patients exhibited global 3' untranslated region (UTR) shortening due to the different usage of polyadenylation sites (PASs). The APA regulator CSTF3 was the most significantly upregulated gene in epithelial cells of platinum-resistant OC. CSTF3 knockdown increased the sensitivity of OC cells to platinum. The lncRNA NEAT1 has two isoforms, short (NEAT1_1) and long (NEAT1_2) transcript, because of the APA processing in 3'UTR. We found that CSTF3 knockdown reduced the usage of NEAT1 proximal PAS to lengthen the transcript and facilitate the expression of NEAT1_2. Downregulation of the expression of NEAT1 (NEAT1_1/_2), but not only NEAT1_2, also increased the sensitivity of OC cells to platinum. Overexpressed NEAT1_1 reversed the platinum resistance of OC cells after knocking down CSTF3 expression. Furthermore, downregulated expression of CSTF3 and NEAT1_1, rather than NEAT1_2, was positively correlated with inactivation of the PI3K/AKT/mTOR pathway in OC cells. Together, our findings revealed a novel mechanism of APA regulation in platinum-resistant OC. CSTF3 directly bound downstream of the NEAT1 proximal PAS to generate the short isoform NEAT1_1 and was conducive to platinum resistance, which provides a potential biomarker and therapeutic strategy for platinum-resistant OC patients. - Source: PubMed
Publication date: 2024/06/19
Luo XinWei QinglvJiang XiaoyanChen NingxuanZuo XinzhaoZhao HongyanLiu YujiaoLiu XiaoyiXie LingcuiYang YuLiu TaoYi PingXu Jing - In gestational diabetes mellitus (GDM), adipose tissue undergoes metabolic disturbances and chronic low-grade inflammation. Alternative polyadenylation (APA) is a post-transcriptional modification mechanism that generates mRNA with variable lengths of 3' untranslated regions (3'UTR), and it is associated with inflammation and metabolism. However, the role of APA in GDM adipose tissue has not been well characterized. In this study, we conducted transcriptomic and proteomic sequencing on subcutaneous and omental adipose tissues from both control and GDM patients. Using Dapars, a novel APA quantitative algorithm, we delineated the APA landscape of adipose tissue, revealing significant 3'UTR elongation of mRNAs in the GDM group. Omental adipose tissue exhibited a significant correlation between elongated 3'UTRs and reduced translation levels of genes related to metabolism and inflammation. Validation experiments in THP-1 derived macrophages (TDMs) demonstrated the impact of APA on translation levels by overexpressing long and short 3'UTR isoforms of a representative gene LRRC25. Additionally, LRRC25 was validated to suppress proinflammatory polarization in TDMs. Further exploration revealed two underexpressed APA trans-acting factors, CSTF3 and PPP1CB, in GDM omental adipose tissue. In conclusion, this study provides preliminary insights into the APA landscape of GDM adipose tissue. Reduced APA regulation in GDM omental adipose tissue may contribute to metabolic disorders and inflammation by downregulating gene translation levels. These findings advance our understanding of the molecular mechanisms underlying GDM-associated adipose tissue changes. - Source: PubMed
Publication date: 2024/03/15
Chen BingnanChen XuyangHu RuohanLi HongliWang MinZhou LinweiChen HaoWang JianqiZhang HanwenZhou XiaoboZhang Hua