GRB2 _ ASH
- Known as:
- GRB2 _ ASH
- Catalog number:
- NB100-65848
- Product Quantity:
- 2 ml
- Category:
- -
- Supplier:
- ACR
- Gene target:
- GRB2 _ ASH
Related genes to: GRB2 _ ASH
- Gene:
- GRB2 NIH gene
- Name:
- growth factor receptor bound protein 2
- Previous symbol:
- -
- Synonyms:
- NCKAP2
- Chromosome:
- 17q25.1
- Locus Type:
- gene with protein product
- Date approved:
- 1994-03-04
- Date modifiied:
- 2016-10-05
Related products to: GRB2 _ ASH
Related articles to: GRB2 _ ASH
- Thymocyte selection is essential for establishing the T cell repertoire, maintaining self-tolerance and preventing autoimmunity. Themis, the archetypal member of a metazoan protein family defined by CABIT domains, centrally regulates this process by integrating T cell receptor (TCR) signalling. Themis has been proposed to constitutively partner with the multifunctional adaptor Grb2, yet the structural and mechanistic basis of this assembly has remained enigmatic. Here, we use Cryo-EM to reveal how the tandem CABIT domains and proline-rich sequence of Themis cooperatively engulf the C-terminal SH3 domain of Grb2, while the unbound domains of Grb2 remain poised to recruit additional binding partners. Furthermore, we uncover inherent interdomain flexibility in unbound Themis that resolves upon Grb2 binding. Structure-guided mutations abrogate the Themis-Grb2 interaction and fail to regulate the tyrosine phosphatase SHP-1 after TCR stimulation, recapitulating the phenotype of Themis-deficient cells. Our findings define the Themis-Grb2 complex as a dynamic structural hub in T cell signalling. - Source: PubMed
Publication date: 2026/05/20
Clancy Danielle MSanz-Sanjuan AlbaGilis ElisabethTougaard PeterVelghe ImkeVan Droogenbroeck YanaFelix JanBloch YehudiCuadrado Álvaro FuronesMerceron RomainSchenck StephanVandenabeele PeterBrunner Janine DTaghon TomElewaut DirkSavvides Savvas N - Sorafenib resistance remains a major therapeutic challenge in advanced hepatocellular carcinoma (HCC). This study aimed to investigate the role of growth factor receptor-bound protein 2 (GRB2) in sorafenib resistance of HCC cells under hypoxic conditions and to elucidate the underlying molecular mechanisms involving the PI3K/AKT signaling pathway. - Source: PubMed
Publication date: 2026/05/06
He JiaqianKong YinzhiWang YunyongFang QiaolingWu HemengDu KeweiLuo YuzhenTan JinnaYin HuiLin HongshengLi Mingfen - Pregnancy-related breast cancer is relatively rare, but its incidence is increasing as the age of childbearing advances. The impact of placenta-specific microRNAs (miRNAs) derived from the chromosome 19 miRNA cluster (C19MC) on pregnancy-associated breast cancer is unclear. Nuclear protein high mobility group box 3 (HMGB3) plays a role in cancer progression. This study examined the effects of placenta-specific C19MC miRNAs on the cancer-related gene HMGB3 in the human breast cancer cell line MCF-7. - Source: PubMed
Sato AiTakei HiroyukiNoguchi SyunyaTakizawa Toshihiro - Lung adenocarcinoma (LUAD) is the main histologic subtype of lung cancer, and its incidence is on the rise. However, since the vast majority of patients are already in advanced stages at the time of diagnosis, their 5-year survival rate is only 15%, so it is urgent to explore the mechanism of the development of LUAD and improve the survival time of patients. Interleukin-11 (IL-11), a member of the IL-6 cytokine family, has an influential role in the development and progression of a variety of tumors, but the specific molecular mechanisms that promote the malignant progression of LUAD are unknown. Here, we found that the IL-11-induced activation of Akt, Erk, and STAT3 could be inhibited by knocking out the expression of Gαi1/3. In contrast, overexpression of Gαi1/3 could enhance IL-11-induced signaling. The binding of Gαi1/3 to GP130 mediates IL-11-induced downstream activation of Akt-mTOR, Erk, and STAT3, which requires recruitment of Grb2-associated binding protein 1 (Gab1). In LUAD cells, shGαi1/3 inhibited cell growth, proliferation, and migration as well as blocked the tumor-promoting ability of IL-11. However, overexpression of Gαi1/3 enhanced the IL-11-induced cell growth, proliferation, and migration. ShGαi1/3 also inhibited the proliferation of LUAD cells in vivo. Overall, the findings of this study demonstrate the Gαi1 and Gαi3 are critical for IL-11 signal transduction. Moreover, we reveal that Gαi1 and Gαi3 are highly expressed and associated with poor overall survival in lung adenocarcinoma and may thus act as potential therapeutic targets in LUAD. These results provide a novel therapeutic strategy for LUAD patients with upregulated IL-11 expression. - Source: PubMed
Publication date: 2026/04/25
Luo GaomengHu WenxuanYang JianLu ZhengCui YuanZeng WeibiaoDing HaoLi QifanChen ZhikeTong XinDing ChengXu ChunZhao Jun - Receptor tyrosine kinase signaling is initiated by extracellular ligand binding, which drives the formation of membrane-protein assemblies that activate intracellular signal transduction. Accurately resolving the molecular composition of these assemblies in situ remains challenging due to their nanoscale dimensions and intrinsic heterogeneity. Here, we introduce a single-molecule super-resolution imaging and analysis workflow designed to resolve and quantitatively characterize individual membrane-protein assembly sites in cells. We apply this approach to the nanoscale organization of the epidermal growth factor receptor (EGFR) and its adaptor protein Grb2 following stimulation with the native ligand epidermal growth factor. As activation progresses, we observe a reduction in EGFR density at the plasma membrane, a progressive accumulation of Grb2 at EGFR assembly sites, and an increase in both dimeric and higher-order oligomeric EGFR. The experimental and analytical framework presented here is broadly applicable to the study of diverse membrane-protein assemblies. - Source: PubMed
Kaminer AlexandraLi YunqingBarth Hans-DieterDietz Marina SHeilemann Mike