Adam2
- Known as:
- Adam2
- Catalog number:
- 033178A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- Adam2
Related genes to: Adam2
- Gene:
- ADAM2 NIH gene
- Name:
- ADAM metallopeptidase domain 2
- Previous symbol:
- FTNB
- Synonyms:
- PH-30b, PH30, CT15
- Chromosome:
- 8p11.22
- Locus Type:
- gene with protein product
- Date approved:
- 1996-10-26
- Date modifiied:
- 2018-05-03
Related products to: Adam2
Related articles to: Adam2
- The interaction between sperm and the zona pellucida (ZP), which involves the binding of sperm surface ligands to complementary carbohydrates on the ZP, is the initial direct contact between gametes and is crucial for subsequent gamete fusion and successful mammalian fertilization. In this study, we show that Garin3 is a critical gene for sperm-oocyte ZP binding. Garin3 expression begins during the acrosomal stage of spermatogenesis, and the gene is highly expressed in the male reproductive system. We created a Garin3 gene-knockout mouse model, and spermatozoa lacking Garin3 exhibited only mild morphological changes and showed no deficiency in motility; however, their ability to bind to the oocyte ZP was severely impaired and this ultimately resulted in failure of in vitro fertilization. These findings suggest that Garin3 is not directly involved in sperm-ZP binding and that it instead indirectly affects such binding by regulating the expression and localization of acrosome-related proteins, including RAB2, SPACA1, ADAM2, and ACR. Notably, fertilization failure was effectively rescued by bypassing the ZP-binding step by removing the ZP altogether or by performing intracytoplasmic sperm injection (ICSI). In human sperm samples, the expression levels of GARIN3 were significantly reduced in sperm with poor fertilization potential compared to sperm with normal fertilization potential, and this was consistent with the phenotypic observations in animal models. Our findings highlight the crucial role of Garin3 in fertilization and the potential molecular mechanism behind its effects. - Source: PubMed
Publication date: 2026/05/16
Chen ZhixianXie SiyuanZou GuoweiZhu HongyingWang LeiSun XiaoxiMu JianSang Qing - ADAM5 is a testis-specific transmembrane protein whose role in male fertility remains poorly understood. In this study, we report that Adam5 knockout (KO) male mice are severely subfertile, despite exhibiting normal testicular morphology, sperm structure, and motility. Adam5 KO sperm failed to transit the uterotubal junction (UTJ) and displayed severe defects in zona pellucida (ZP) binding, phenotypes that resemble those observed in Adam2 and Adam3 knockout mice. Western blot analysis revealed a significant reduction in the levels of ADAM2 and ADAM3 in Adam5 KO spermatozoa, supporting the previous finding that ADAM5 interacts with these proteins to form a complex. Additionally, Adam5 KO spermatozoa exhibited reduced binding to extracellular matrix (ECM) components, including Laminin I and Fibronectin. These findings suggest that ADAM5 plays a crucial role in sperm ECM interaction, a process likely critical for successful UTJ transit and ZP binding. While ADAM5 is a pseudogene in humans, our results provide valuable insights into the function of ADAM family proteins in mammalian reproduction. - Source: PubMed
Mashiko DaisukeTonai ShingoIkawa Masahito - Testicular tissue displays the most complex transcriptome across all tissues, with over 2000 genes exhibiting testis-enriched expression patterns. However, the functional ambiguity of such genes limits our understanding of spermatogenesis and male fertility. Here, we reanalyzed testicular gene expression profiles from patients with impaired spermatogenesis and identified that reduced expression of the testis-specific gene C3ORF22 was correlated with spermatogenic defects in humans. We showed that the murine ortholog BC048671 was predominant expressed in round spermatids, and its protein is present in spermatozoa. BC048671 knockout (KO) mice exhibited normal fertility, sperm morphology, and sperm motility. Intriguingly, RNA-Seq analysis revealed that BC048671 was the most markedly dysregulated gene in KO testes. Although proteomic analysis reveals the down-regulated ADAM family members (e.g., ADAM28 and ADAM2) in BC048671-null sperm, expression of the key downstream effector ADAM3 remained unaffected. These findings indicate that BC048671/C3ORF22 exhibits functional redundancy in spermatozoa. Although C3ORF22 is dispensable for male fertility, we consider it essential to report such negative results to guide researchers to prioritize efforts toward genes critical for human fertility. - Source: PubMed
Publication date: 2025/10/11
Du ShiyueLiu YongfuWang LeShi LiuliuZhao XiaoyingYang ZhongchengYuan WeiDeng XiaoyingLiu JiahuaFeng ShengleiChen Rui - To study the effect of the mouse Fertilin peptide, on mouse in vitro and in utero embryo development. - Source: PubMed
Publication date: 2025/08/08
Favier SophieL'Hostis AudreyPierre RémiLagoutte IsabelleVicaut EricVaiman DanielWolf Jean Philippe - Sacral neuromodulation (SNM) implantable devices such as the InterStim® device by Medtronic carry a small risk of stimulatory lead migration. In very rare cases migrated leads can injure the rectal wall resulting in a full-thickness perforation. The sequela of rectal perforation by SNM leads may have varied clinical presentation, and can remain undiagnosed without appropriate suspicion, increasing the risk for further complications. - Source: PubMed
Publication date: 2025/07/04
Brown Adam ELambie CadenChoudry Mouneeb MDurant Adri MLu Patricia GRappaport Douglas E