C4orf49
- Known as:
- C4orf49
- Catalog number:
- 003097A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- C4orf49
Related genes to: C4orf49
- Gene:
- MGARP NIH gene
- Name:
- mitochondria localized glutamic acid rich protein
- Previous symbol:
- C4orf49
- Synonyms:
- OSAP, CESP-1, HUMMR
- Chromosome:
- 4q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 2008-11-28
- Date modifiied:
- 2015-11-13
Related products to: C4orf49
Related articles to: C4orf49
- Silicosis is the most common occupational lung disease caused by respirable crystalline silica inhalation, with limited therapeutic options. Cellular senescence plays a critical role in the pathogenesis of lung diseases, while the role of senescent macrophages in silicosis remains unclear. Single-cell RNA sequencing (scRNA-seq) of healthy and silicosis human and mouse lung tissues revealed that activating transcription factor 3 (ATF3)-mediated macrophage senescence is closely linked to silicosis progression. Mechanistically, Sirtuin 6 (SIRT6)-mediated ATF3 deacetylation enhanced its nuclear transport and subsequently activated mitochondria-localized glutamic acid-rich protein (MGARP) transcription, thereby causing mitochondrial dysfunction and macrophage senescence. Senescent macrophages promoted fibroblast activation via the secreted phosphoprotein 1 (SPP1)-cluster of differentiation 44 (CD44) signaling pathway. Furthermore, the nuclear transport protein importin α and the molecular chaperone protein heat shock protein 70 (HSP70) competitively bound to ATF3, preventing its lysosomal degradation while promoting its nuclear import during macrophage senescence. Moreover, the small-molecule inhibitor Itraconazole, which targets the binding site of ATF3 and importin α, could reduce ATF3 nuclear entry, macrophage senescence, and pulmonary fibrosis (PF). Collectively, our study provided insights into the mechanism by which deacetylated ATF3 facilitates silicosis progression via increased nuclear transport and macrophage senescence, and indicated potential therapeutic targets for PF. - Source: PubMed
Publication date: 2026/05/20
Cheng DeminBu WenxiaWang FengxuJin YueyuanLiu RongzhuZhao RuiWang XuehaiJiang MengnaShen JinpingCheng XinhangChen ZumingZhu LiLi JinlongGe ZhenzhongMiao ShichenXu HaotianZhou XiaoyuWang DongmingZhao Xinyuan - MicroRNAs (miRNAs) play an important role in the occurrence of non-obstructive azoospermia (NOA). Nevertheless, there is still a lack of research on the molecular mechanisms by which miRNAs regulate target genes to mediate NOA at present. In this study, we obtained NOA-related miRNA datasets from the GEO database and applied differential expression matrices combined with weighted correlation network analysis (WGCNA) and LASSO regression to identify four key miRNAs. Based on the miRDB database, the target genes of these miRNAs were predicted and intersected with the differentially expressed genes (DEGs) in the NOA transcriptome datasets. This intersection resulted in the identification of 18 DEGs. The spermatogenesis score model revealed a significant positive correlation between the overall expression level of these 18 DEGs and spermatogenesis scores, suggesting their potential involvement in NOA development. These 18 DEGs were subsequently incorporated into machine learning, leading to the identification of four hub genes with high diagnostic value: , , , and . In the NOA mouse model, and were upregulated, whereas and were downregulated, consistent with the expression trends observed in the NOA datasets. These findings indicate that , , , and may serve as novel biomarkers for NOA, providing a theoretical and experimental foundations for elucidating its mechanisms and improving clinical diagnosis. - Source: PubMed
Li Zhi-HongChen Miao-QiYuan Xiao-JunHuang Hua-JunHuang Wan-TingZhou Piao-YanZeng ChenFeng XunuoYang Luo-YaoHuang Shu-QiangTan Cui-YuChen Cai-RongYan Qiu-Xia - Lion-head goose is a large-sized breed native to Guangdong Province, China, exhibits remarkable capacity for fatty liver production under overfeeding conditions and is highly valued by local farmers and consumers. However, the molecular mechanisms driving fatty liver development in this breed are still unknown. In this study, we evaluated liver weight differences between normally fed and overfed Lion-head geese and further examined sex-specific differences following overfeeding. Overfeeding significantly increased liver weight more than 340%, and males possess a stronger capacity for lipid deposition under the same feeding regimen compared with females. RNA-Seq analysis identified 1476 differentially expressed genes (DEGs) shared by both sexes, which were mainly enriched in lipid and energy metabolism, oxidative stress, and mitochondrial pathways. In addition, 627 male-specific and 420 female-specific DEGs revealed sex-dependent differences, with males showing stronger transcriptional regulation and females exhibiting enhanced antioxidant and detoxification responses. Weighted gene co-expression network analysis (WGCNA) revealed 320 co-hub genes enriched in lipid and energy metabolism in overfeeding-induced fatty liver, along with 9 co-hub genes related to sex differences. Alternative splicing (AS) analysis detected 131 differentially spliced genes (DSGs). Integration of both approaches identified 7 overlapping genes, (Hyccin PI4KA lipid kinase complex subunit 2), (Amylo-Alpha-1,6-Glucosidase and 4-Alpha-Glucanotransferase), (Coiled-coil domain containing 62), (Immunoglobulin superfamily member 5), (Mitochondria-localized glutamic acid-rich protein), (Cluster of Differentiation 80), and (Folylpolyglutamate synthase), as potential key regulators. These findings provide new insights into transcriptional and post-transcriptional regulation of overfeeding-induced fatty liver in geese. - Source: PubMed
Publication date: 2025/12/30
Fu JingLan YezhiLiang YuwenYang XiaoguangTang RuizeWang YuchuanLuo YabiaoLiu Chunpeng - The causes of premature ovarian failure (POF) as a result of high-fat and high-sugar (HFHS) diets have not been studied systematically or in depth. In this study, we used single-cell RNA-seq (scRNA-seq) and molecular pathology experimental techniques to systematically analyze the ovarian landscape in HFHS diet-induced POF in mice. An HFHS diet decreased levels of AMH and E2 and induced a significant amount of follicular atresia in mice, according to the enzyme-linked immunosorbent assay (ELISA) and pathology results. The scRNA-seq results also showed that the number of Krt19 + epithelial cell, Csmd1 + cumulus cell, Mgarp + thecal cell, and mt-CO1 + granulosa cell clusters was much higher in the ovarian tissues of HFHS-POF mice than it was in the control group. According to the KEGG analysis, the differentially transcribed genes in these three cell clusters in different groups were chiefly involved in multiple signal transduction pathways, and their overlapping signaling pathways included oxidative phosphorylation. Immunofluorescence staining and qPCR showed that expression levels of the oxidative phosphorylation signaling pathway were significantly lower in the control group than they were in the thecal cell, epithelial cell, and stromal cell clusters of the HFHS-POF mice. To the best of our knowledge, this study is the first to report the application of scRNA-seq to analyze the ovarian landscape of HFHS-fed mice and to verify that the HFHS diet induced POF in mice by activating the oxidative phosphorylation signal transduction pathway in the thecal cell, granulosa cell, and cumulus cell clusters in mouse ovarian tissues. - Source: PubMed
Publication date: 2025/09/18
Li KaiWen YichaoCui ZeyuLing LeleWang ChunxiaZhang BimengLiu Te - The feasibility of preparing a methionine/glucose-derived Amadori rearrangement product (MG-ARP) under aqueous and solid states was investigated. The results showed that vacuum dehydration in an aqueous medium and solid-phase reaction of freeze-dried powder could overcome the energy barrier against the dehydration of N-substituted glycosylamine by removing moisture, thereby facilitating intramolecular dehydration and improving conversion of MG-ARP compared with the atmospheric sealing reaction. A reduction in humidity during solid-phase reaction enhanced the formation of MG-ARP. Vacuum dehydration, which retained a small amount of moisture, improved MG-ARP formation without generating numerous downstream by-products, while the solid-phase reaction favored both MG-ARP formation and its degradation. Weakly alkaline primarily accelerated MG-ARP production rather than browning formation during vacuum dehydration. Increasing the vacuum dehydration temperature promoted methionine/glucose (Met/Glc) dehydration to generate MG-ARP more strongly than it promoted MG-ARP degradation to form 3-deoxyglucosone (3-DG). - Source: PubMed
Publication date: 2025/09/06
Deng ShibinCui HepingHussain ShahzadHayat KhizarXiao JiahuiZhang Xiaoming