AXIN2
- Known as:
- AXIN2
- Catalog number:
- 002329A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- AXIN2
Related genes to: AXIN2
- Gene:
- AXIN2 NIH gene
- Name:
- axin 2
- Previous symbol:
- -
- Synonyms:
- MGC126582, DKFZp781B0869
- Chromosome:
- 17q24.1
- Locus Type:
- gene with protein product
- Date approved:
- 1998-09-17
- Date modifiied:
- 2019-04-23
Related products to: AXIN2
Related articles to: AXIN2
- Sulfometuron-methyl (SM), as a sulfonylurea herbicide, is widely used in agricultural production. In recent years, it has also been employed in the control and management of Spartina alterniflora in some coastal areas of China. Its potential risks to aquatic ecosystems have drawn increasing attention. However, the current data on the systematic toxicity assessment of SM in model organisms is still very limited. This study used zebrafish () as an in vivo model to systematically evaluate the toxic effects of SM on early embryonic development and focused on the mediating mechanism of the Wnt/β-catenin signaling pathway in it. Zebrafish embryos were exposed to different concentrations (10, 20, 40 mg/L) of SM starting from 1.75 hpf until 72 hpf. The results showed that SM significantly increased the embryo mortality rate, decreased the hatching rate, and induced developmental malformations such as shortened body length and yolk sac edema in a concentration-dependent manner. At the mechanism level, SM exposure significantly reduced the number of H3P-positive mitotic active cells in the embryos, suggesting that fewer cells entered mitosis; meanwhile, the activities of superoxide dismutase (SOD) and catalase (CAT) showed compensatory changes, indicating that oxidative stress was induced in the embryos, which may contribute to subsequent cellular damage. qRT-PCR analysis further revealed that SM exposure downregulated the transcriptional levels of cell cycle-related genes (, , ), while upregulating the expression of apoptosis-related genes (, , , ), suggesting that cell cycle arrest and the dysregulation of the endogenous apoptotic pathway were activated. Additionally, SM exposure could dysregulate the Wnt/β-catenin signaling pathway, specifically manifested as significant upregulation of target genes and , and significant downregulation of the negative regulatory factor . Using the Wnt/β-catenin pathway-specific inhibitor IWR-1 for intervention could partially reverse the embryonic developmental retardation phenotypes induced by SM, providing additional evidence for the mediating role of this pathway in the developmental toxicity of SM. In summary, this study systematically elucidates the multi-level molecular mechanism by which SM induces oxidative stress and cell apoptosis through the abnormal dysregulation of the Wnt/β-catenin signaling pathway, reduced mitotic activity, and ultimately drives the toxicity of zebrafish embryo development. This provides new experimental evidence and a theoretical basis for the aquatic ecological risk assessment of SM. - Source: PubMed
Publication date: 2026/09/20
Zhang XiaomeiLiu JinlongGuo YongxinZhong JunDong YanCheng ShuoqianLi ZiweiQiang WeidongWang Huiyan - Gastric gland cells originate from two proliferative niches: the main rapid one in the isthmus, and the slow secondary one in the base. However, while nutrition is dependent on breastfeeding, the proliferative niches are not established, and proliferation occurs along the gland. Currently, we investigated the influence of early weaning (EW) on shaping gastric proliferative niches and their signaling. Wistar rats were separated into suckling (S) and EW groups on postnatal day (PND) 15 for gastric mucosa collection. BrdU was administered (PND 14 and 15) and as it diluted between daughter-cells we evaluated migration and differentiation. In parallel EdU incorporation was used to identify cells during S phase. Through these tools, we studied the different profiles of BrdU/EdU dual labeling at 18, 21 and 30 PND. At 18 PND, EW decreased BrdU+ population (p < 0.0001 vs. S group) in the upper and bottom gland areas (p < 0.05). The BrdU + /EdU+ cells were concentrated in the upper gland, where we recorded a larger EdU+ population after EW (p < 0.05). On PND 30, EW decreased BrdU+ cells in the gland (p < 0.05), whereas EdU+ cells were more numerous in the isthmus. At 18 PND, EW reduced Wnt3, Notch1, and Notch2 expressions (p < 0.05), while increasing Bmp2. In contrast, at 60 PND, Axin2, Notch1, and Notch2 transcripts were elevated after EW (p < 0.05). Notch1 and Notch2 distribution indicated that EW might shift Notch2 to the upper gland, potentially affecting the fate of progenitor cells in pups. In silico analyses showed that zymogenic cells are in a renewal-promoting environment, while parietal cells contribute to differentiation and signaling. Therefore, EW restricted the proliferative activity to the isthmus, altering the spatiotemporal expression of genes involved in stemness and differentiation, and reshaped the distribution of Notch receptors, without affecting the distribution of its downstream effector Hes1. These spatially organized molecular landscapes are crucial for proper epithelial renewal and function, and our data suggest that early nutritional disruption reprograms these niches with long-term consequences. - Source: PubMed
Rattes Isadora CamposCosta Marcos Aurélio Santos dade Oliveira Viviane RosaGama Patrícia - Merkel cell polyomavirus (MCPyV) is the established oncogenic driver of Merkel cell carcinoma (MCC), but its role in other malignancies is under investigation. Although MCPyV DNA has been detected in colorectal cancer (CRC) specimens, its biological relevance and contribution to colorectal tumorigenesis remain unclear. In this observational study, we investigated MCPyV prevalence, load and molecular status in 122 CRC specimens and peritumoral tissues. In addition, to extend beyond DNA detection, transcriptional profiling was performed to assess early (LTAg) and late (VP1) transcripts, and viral microRNAs. Furthermore, the mRNA expression levels of key Wnt signaling components, including CTNNB1, MYC and CCND1 were examined. AXIN2 was also used as a surrogate marker of pathway activation. Our findings revealed similar MCPyV prevalence and load between tumor and adjacent healthy tissues, suggesting a wide colonization of the colorectal mucosa. Sequencing analyses showed canonical NCCR and VP1 structures, and full length LTAg, with no evidence of viral integration. These data, combined with the detection of both early and late transcripts, indicate an episomal and transcriptionally active state in colorectal tissues. Notably, virus-positive tissues exhibited an over-expression of CTNNB1and its target genes across all clinical subgroups, regardless of anatomical site or tumor stage. Our results are congruent with the establishment of persistent MCPyV infection in the colorectal mucosa, potentially characterized by transcriptionally active episomal genomes. Furthermore, upregulation of the Wnt/β-catenin cascade in virus-positive tissues suggests that MCPyV may exploit this host pathway to drive cellular proliferation. A limitation of this study is lack of comparison to colorectal mucosa in patients without CRC. Overall, these findings are consistent with widespread viral colonization of the colorectal mucosa in a subset of CRC patients, and raise the hypothesis that MCPyV may modulate host signaling pathways relevant to colorectal biology. Whether MCPyV plays any etiological role in colorectal carcinogenesis, or whether its detection is coincidental, cannot be determined from the present observational data. - Source: PubMed
Publication date: 2026/09/24
Passerini SaraDolci MariaMessina SaraCaterina Valentina AlyssaSignorini LuciaCamurri FrancescaGraziani MarcoGallo GiorgiaNardo Giovanni DiDelbue SerenaPietropaolo Valeria - Zhuche Pill (ZCP), a traditional Chinese medicine formula first documented in Qianjin Yaofang during the Tang Dynasty, has been used for centuries to treat dysentery-associated gastrointestinal disorders, including abdominal pain, diarrhea, hematochezia, and tenesmus. Despite the long-standing clinical application of ZCP, the pharmacological basis underlying its therapeutic effects remains incompletely understood. - Source: PubMed
Publication date: 2026/09/24
Zhang TaoCong BeiyangYang ZhicongHuang NiLin YikeMai ChutianZhang EnxinHu YingZhang Xiaojun - Ischemic stroke is a leading cause of death and disability, with limited therapies for neurological recovery. Buyang Huanwu Decoction (BYHWD) is a traditional Chinese medicine formula used for stroke, but its effects on endogenous neurogenesis and the underlying mechanisms remain incompletely defined. - Source: PubMed
Publication date: 2026/09/22
Xu JiadongLiu ShuyanChen HaoweiSang TingtingZhang YaniSheng XuanYang YanFang YanXu LanxiChu Lisheng