ANPEP
- Known as:
- ANPEP
- Catalog number:
- 001683A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ANPEP
Related genes to: ANPEP
- Gene:
- ANPEP NIH gene
- Name:
- alanyl aminopeptidase, membrane
- Previous symbol:
- CD13, PEPN
- Synonyms:
- LAP1, gp150, p150
- Chromosome:
- 15q26.1
- Locus Type:
- gene with protein product
- Date approved:
- 1989-02-28
- Date modifiied:
- 2016-10-05
Related products to: ANPEP
Related articles to: ANPEP
- The carcinogenic relevance of environmental contaminant bisphenol A (BPA) to colorectal cancer (CRC) has gained growing attention, yet the molecular networks potentially linking BPA exposure to CRC remain incompletely characterized. This study integrated network toxicology and machine learning to predict candidate molecular targets and putative signaling networks associated with BPA-correlated colorectal carcinogenesis. The study's methodology involved an initial differential expression screening across several CRC transcriptomic datasets to establish a disease-specific gene signature. Subsequently, a multi-tiered computational strategy was employed: network toxicology was used to map potential BPA-protein interactions, machine learning algorithms were applied to distill a minimal set of high-impact targets, and molecular docking simulations provided atomic-level validation of the proposed binding events. We identified 53 overlapping genes between predicted BPA-interacting proteins and CRC-related transcripts. Machine learning screening further filtered a 12-gene panel with favorable predictive performance for CRC status, including MET, SORD, DPEP1, KIT, RIPK2, SET, HSP90AB1, DBF4, MMP1, MMP12, ANPEP, and GLA. Molecular docking simulations predicted stable binding interactions between BPA and the protein products encoded by these 12 genes. This study delineates a specific gene network potentially targeted by BPA to promote CRC pathogenesis. The machine learning-derived 12-gene signature, interpreted as CRC-associated genes overlapping with predicted BPA targets and supported by in silico molecular docking, offers valuable insights into the molecular basis of BPA-associated colorectal carcinogenesis and presents candidate targets for subsequent experimental validation. - Source: PubMed
Publication date: 2026/08/16
Li XiaoxuanMai GenningXiao YuexiZhao RuijunHuang XiyueLi JunxiXiao YuchenDeng ZhujunLi MiaoXiong Wenjun - Liver metastasis is the leading cause of death in pancreatic ductal adenocarcinoma (PDAC), yet its molecular drivers remain poorly defined. Through integrated transcriptomic and proteomic screening of clinical specimens, we identify the membrane protein Cadherin-Related Family Member 2 (CDHR2) as a key orchestrator of hepatic colonization in PDAC. CDHR2 was upregulated in primary tumors versus adjacent tissues, further elevated in liver metastasis versus matched primary tumors, and markedly enriched in patient-derived plasma extracellular vesicles (EVs), peaking in patients with liver metastasis. Knockdown of CDHR2 in human PDAC cells suppressed proliferation, migration, and invasion, and in orthotopic models attenuated primary tumor growth, remodeled the tumor microenvironment, and reduced liver metastatic burden. Mechanistically, CDHR2 associated with PDK1 and promoted activation of the PDK1-AKT (Thr308) axis to drive proliferation. In turn, AKT activity correlated with the packaging of CDHR2 into EVs, coupling intrinsic signaling to EV cargo loading. Tumor-derived, CDHR2-enriched EVs formed a complex with Aminopeptidase N (ANPEP) on liver sinusoidal endothelial cells (LSECs), disrupting endothelial junctions and enhancing tumor-cell adhesion to enable transendothelial migration. Consistently, ANPEP inhibition or liver-specific knockdown curtailed hepatic colonization, whereas priming with CDHR2 EVs accelerated it. Finally, systemic delivery of EV-encapsulated siCDHR2 suppressed established primary tumors and markedly reduced liver metastasis. Collectively, our findings define a tumor-intrinsic CDHR2-PDK1-AKT (Thr308) axis coupled to an exosomal CDHR2-ANPEP endothelial-priming program and provide proof-of-concept for exosomal siCDHR2 as a candidate strategy against PDAC liver metastasis. - Source: PubMed
Publication date: 2026/08/04
Wang RuiWang YiXiao ShutingLiao ShuilinLiu SongsongChen XiZhou YinShan JingjingSong QibinWang HuaizhiChen QiangDong Ming - Can ANPEP-based magnetic activated cell sorting (MACS) be used to isolate and functionally enrich human steroidogenic theca interna cells from small antral follicle membranes? - Source: PubMed
Publication date: 2026/07/23
Jensen Lea BejstrupJohannsen Malene LouiseBøtkjær Jane AlrøRonnefeld Veng AnnaTryde Macklon KirstenTønnes Pedersen AnetteStyrishave BjarneKristensen Stine Gry - Alanyl aminopeptidase (ANPEP) has been implicated in various cancers, but its specific role in gastric adenocarcinoma (GC) remains incompletely understood. This study analyzed ANPEP gene expression in gastric cancer (GC), peritumoral tissue (PTT), metaplasia (M), and normal tissue (N). Total RNA was extracted, libraries were prepared and sequenced on the Illumina NextSeq 500. Data were processed using the nf-core/rnaseq pipeline. Transcript quantifications were imported with tximport and normalized using DESeq2. Differential expression (|log₂FC| > 1; adj. p < 0.05) and Kruskal-Wallis tests identified key genes. ANPEP was significantly upregulated in GC, PTT, and M compared to normal tissue (p < 0.01), suggesting its involvement in early mucosal transformation and malignant progression. Heatmap and pathway enrichment analysis revealed upregulation of genes related to immune function and oxidative stress, indicating an immunosuppressive and apoptosis-resistant tumor microenvironment. Correlation analyses identified strong positive associations between ANPEP and genes involved in cytoskeletal remodeling, immune modulation, and metabolic regulation, suggesting that ANPEP supports both the invasive potential of tumor cells and the establishment of an immunosuppressive, therapy-resistant niche. These findings position ANPEP as a promising biomarker for early detection and a candidate for targeted therapies. - Source: PubMed
Publication date: 2026/07/20
Araújo Taíssa Maíra ThomazRodrigues Bianca de Fátima Dos ReisSilva Jessica Manoelli Costa daRemígio Myrth Soares do NascimentoMoreira Fabiano CordeiroCasseb Samir Mansour MoaresBarra Williams FernandesIshak GeraldoAnaissi Ana Karyssa MendesMagalhães LeandroVidal AmandaMourão Ronald Matheus da SilvaTeixeira Eliel BarbosaPereira DiegoSilva Valéria Cristiane Santos daAvelar Daniel de SouzaSilva Rubem FerreiraSantos Ândrea Kely Ribeiro DosDemachki SamiaMarques Livia Erika CarlosBurbano Rommel RodriguezAssumpção Paulo Pimentel de - Deer antlers represent one of the few mammalian organs capable of complete regeneration, yet the underlying genomic regulatory mechanisms remain incompletely understood. Transposable elements (TEs), as key drivers of genome evolution and gene regulation, may play pivotal roles in the evolution of complex traits. This study aimed to systematically investigate the evolutionary dynamics of TEs in sika deer genome and to elucidate their potential regulatory functions in the development of antler by integrating comparative and functional genomics approaches. - Source: PubMed
Publication date: 2026/07/15
Wang QianghuiXing HaihuaMa YukaiSun ZihuiHan RuobingLi Heping