ANAPC5
- Known as:
- ANAPC5
- Catalog number:
- 001554A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ANAPC5
Related genes to: ANAPC5
- Gene:
- ANAPC5 NIH gene
- Name:
- anaphase promoting complex subunit 5
- Previous symbol:
- -
- Synonyms:
- APC5
- Chromosome:
- 12q24.31
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-01
- Date modifiied:
- 2014-08-12
Related products to: ANAPC5
Related articles to: ANAPC5
- The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase that regulates cell cycle progression, but its role in plant-pathogen interactions is poorly understood. Here, we demonstrate that regulation of the ubiquitin-proteasome system, involving APC/C-associated components, is critical for coordinating host cell cycle modulation and cellular homoeostasis required for proper gall formation and successful nematode parasitism. Inhibition of the 26S proteasome with MG132 severely disrupted gall morphology and nematode development, indicating that proteasome activity is required for feeding site establishment and maintenance. To assess the contribution of APC/C associated components involved in proteasome-mediated degradation, we examined the effects of overexpressing APC5, APC7 and APC10 during interactions with the root-knot nematode Meloidogyne incognita and the oomycete Hyaloperonospora arabidopsidis (Hpa). Overexpression of a truncated APC7 variant (APC7-CT), conferred strong resistance to both pathogens, reducing nematode egg mass production by 81% and Hpa conidiospore production by 97%. APC7-CT plants exhibited altered gall morphology, suggesting premature senescence of giant cells. In contrast, overexpression of APC5 or APC10 increased susceptibility to M. incognita, revealing functional divergence among APC/C components. Our findings identify a previously unrecognised role for APC/C-related regulatory pathways in regulating host cellular processes that influence pathogen success and highlight APC7-CT as a potential target for improving resistance to diverse pathogens. - Source: PubMed
Publication date: 2026/09/22
Carneiro Aline KöhnSiqueira Kércya Maria Simões deAnh La HoangPagnotta SophieNascimento Danila Cabral doEloy Nubia BarbosaKeller HaraldHemerly Adriana SilvaEngler Janice de Almeida - Several evolutionarily conserved genes have been reported to be involved in gametogenesis. Y-box binding proteins, which have a conserved nucleotide-binding cold-shock domain, have been reported in both vertebrates and invertebrates. Although homologues of Ybx1 in Drosophila and zebrafish play important roles in oogenesis, the involvement of mouse Ybx1 in gametogenesis or gonadal development remains unclear. In this study, we investigated the female and male fetal gonadal phenotypes in Ybx1 knockout mice. Embryos of Ybx1 knockout mice at E13.5 showed slender gonads compared to those of the controls. Whole-mount gonadal immunostaining revealed a significant decrease in the number of female embryonic germ cells. Immunocytochemistry revealed that YB-1, a protein encoded by Ybx1, is expressed in both germ and gonadal somatic cells. In contrast, transcriptome analysis of germ cells and gonadal somatic cells at E13.5 revealed that deletion of Ybx1 did not cause global changes in gene expression. However, Anapc5 and Rpn1, which have been implicated in germ cell survival, were significantly downregulated in Ybx1 female germ cells. Furthermore, Acta2 and Mmp2, known Ybx1-regulated genes, were also significantly downregulated in Ybx1 female gonadal somatic cells, potentially contributing to germ cell hypoplasia. Collectively, our results suggest that YB-1 may have a role in gonadal development by promoting germ cell survival and gonadal development. - Source: PubMed
Publication date: 2026/05/31
Ikeda ShinyaObata YayoiSotomaru Yusuke - Maxim (DrT) is a well-known traditional medicinal and edible plant with hepatoprotective effects. In this study, crude polysaccharides of DrT (DrTPs) were obtained using the water extraction-ethanol precipitation method. Autoimmune hepatitis (AIH) models of both mice and ALM12 cells were produced by ConA. The serum liver function indexes (AST and ALT) were examined by ELISA, and liver tissue pathological changes were observed by HE staining. The hepatoprotective mechanism of DrTP80 was explored by RNA sequencing and verified by detecting the protein expressions using Western blot. As a result, DrTP80 could significantly reduce AST and ALT levels in the injured liver and ALM12 cells. DrTP80 also obviously improved the hepatopathological changes in liver tissue induced by ConA. Furthermore, RNA sequencing detected significant differences in gene expression, and the functions of differential genes were focused on TNF and IL-17 signaling pathways. Based on these two signaling pathways, 13 differentially expressed genes (Vcam1, Atf6b, Akt1, Irf1, Map2k3, Lcn2, Hsp90ab1, Anapc5, Traf4, Fosl1, Jun, Cxcl5, Nfκbia) among NC, CRC, and FP groups were screened and verified by Western blot. In conclusion, our results demonstrated that DrTP80 can alleviate immune liver damage induced by ConA, and its hepatoprotective mechanism may be related to regulating TNF and IL-17 signaling pathways. Our findings indicated that DrTP80 could be exploited as a healthy food supplement for the treatment of immune liver injury. - Source: PubMed
Publication date: 2026/03/24
Guo MinWei SaixueCheng BiaobiaoLi Xiaodong - The pathophysiology of multiple sclerosis (MS) bears notable similarities to the dysregulated inflammatory response occurring during coronavirus disease 2019 (COVID-19) infection. B cells play a pivotal role among immune cells in the pathogenesis of both these diseases. Consequently, clarifying the molecular mechanism underlying B cell function in COVID-19 and MS is of great significance for formulating more efficient treatment strategies. A comprehensive analysis integrating single-cell RNA sequencing (scRNA-seq), genome-wide association study, and expression quantitative trait locus data from patients with COVID-19 and MS was performed. Gene set enrichment analysis revealed pathways and functional roles associated with the key genes, while pseudotime analysis tracked their expression patterns across different B cell developmental trajectories. The results of scRNA-seq analysis showed that, in comparison with the healthy control group, the proportion of B cells rose in patients with COVID-19 and those with MS. Through differential expression analysis and Mendelian randomization analysis, , , and were identified as risk factors for both COVID-19 and MS, whereas was characterized as a protective factor against these two conditions. The findings of the pseudotime analysis indicated that only had differential expression across different branches of B cells. 's role in promoting immune inflammation and inhibiting metabolism could potentially be linked to the onset and comorbidity of COVID-19 and MS. This emphasizes not only the possible interaction mechanisms between these two diseases but also their clinical significance. - Source: PubMed
Publication date: 2026/03/17
Chen ShupingRuan JunCheng SikaiZheng HuifangChang TianyuBao GuichunZhu ZijingLi XinglinZhao WeiZheng Kunwen - Radiotherapy plays a central role in cancer treatment, and the immunostimulatory effects of radiotherapy have been increasingly recognized. A better understanding of the mechanisms underlying postradiation immune escape is needed to help overcome radioresistance. In this study, we identified that irradiated tumor cells exploit the ANAPC5/GPAA1 axis to elevate surface expression of the "do not eat me" signal CD24, inducing phagocytosis resistance and immune evasion. Mechanistically, radiation inhibited the APC/C complex, reducing ANAPC5-mediated ubiquitination of GPAA1, a catalytic subunit of glycosylphosphatidylinositol (GPI) transamidase. The subsequent accumulation of GPAA1 facilitated GPI anchoring, thereby enhancing CD24 membrane localization. Accordingly, ablation of GPAA1 or CD24 significantly potentiated the local antitumor effects of radiotherapy across multiple preclinical models, dependent on T cells and macrophages. Notably, CD24 deficiency also stimulated abscopal effects, suppressing the growth of nonirradiated tumors. Overall, this study elucidates a mechanism of radiotherapy-mediated upregulation of the innate immune checkpoint CD24, offering perspectives on radiation-induced immune escape and presenting a strategy to improve radiotherapy efficacy. - Source: PubMed
Kong LingyiZhou MinqiYuan WenqianWang YijunLiu XixiWang JiachengZhong WeidongChen QinyanLi PengfeiPu TingtingFeng ZishanZhou ZhiyuanDeng YueWei WenwenYang XiaoMeng JingshuSheng YuhanWan ChaoHuang FangYang KunyuSun Yajie