AMELY
- Known as:
- AMELY
- Catalog number:
- 001521A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- AMELY
Related genes to: AMELY
- Gene:
- AMELY NIH gene
- Name:
- amelogenin Y-linked
- Previous symbol:
- AMGL
- Synonyms:
- -
- Chromosome:
- Yp11.2
- Locus Type:
- gene with protein product
- Date approved:
- 1988-08-31
- Date modifiied:
- 2018-04-26
Related products to: AMELY
Related articles to: AMELY
- This study aimed to explore whether zona-associated residual sperm may contribute to exogenous contamination and cause sex bias in PCR-based sex classification of ovine early-stage embryos, based on the amplification of the gene. Primers targeting the conserved regions of the ovine and genes were designed, and a nested PCR system using AMEL and genes was established. Group A comprised 17 female and 33 male embryos, corresponding to a female-to-male ratio of 0.52:1, whereas Group B comprised 23 female and 27 male embryos, corresponding to a female-to-male ratio of 0.85:1. DAPI staining revealed sperm-morphology structures or structures containing sperm-derived DNA adjacent to the zona pellucida of ovine early-stage embryos. Sequence alignment of PCR amplicons showed consistency with the and reference genes. In conclusion, these results support our research hypothesis that zona-associated residual sperm may serve as a potential source of contamination and interfere with PCR-based embryonic sex determination. - Source: PubMed
Publication date: 2026/09/14
Ma QiangqiangChen YingDong HongWang LiqinFu XuefengAbudureyimu GulimireZhang JiaxinZhang WeiTian JinboWu YangshengLin Jiapeng - - Source: PubMed
Publication date: 2026/09/24
Shrivastav Vivek KumarBhati PraveeshKaitholia KamleshShukla Deepali - Sex-inference in forensic DNA analysis is a critical investigative parameter, commonly relying on the Amelogenin locus, along with the Y-Indel marker (rs2032678) and a limited set of Y-chromosomal markers included in autosomal STR multiplexes and quantitative PCR assays. However, structural rearrangements of the Y chromosome can result in discordant profiles that misidentify phenotypically male individuals as "false females" in standard autosomal kits. We report an exceptionally rare case from central India exhibiting three convergent genetic anomalies in a phenotypic male blood sample: the loss of primary forensic sex markers (AMELY/Y-Indel), X-chromosomal heterozygosity and an extensive Yq deletion. This "triple-failure" haplotype (AMELY-/Y-InDel-/Yq-) represents unprecedented complexity combining South Asian-pattern Yp11.2 microdeletion with massive Yq loss and X-chromosomal diploidy. Moreover, Quantification discordance occurs between Quantifiler Trio and PowerQuant confirmed selective Yq loss, while SRY positivity and NGS validation established male genetic sex. YHRD Release R69 analysis of the partial Yfiler Plus profile revealed no matches worldwide (n = 106,444; RMP <1/106,444, 95% CI up to 1/28,856) or in Eurasian-Indian (n = 1240; RMP <1/1240, 95% CI up to 1/337) and Indian national (n = 1238; RMP <1/1238, 95% CI up to 1/336) databases. The case exposes critical diagnostic limitations of single-target sex typing and Yq-biased quantification in structural variants, underscoring the need for redundant markers (SRY, Yp STRs, multi-Y qPCR targets). Integrated STR/NGS analysis with database frequency assessment remains essential for resolving such "ghost" Y-profiles in forensics. - Source: PubMed
Publication date: 2026/08/29
Shrivastav Vivek KumarBhati PraveeshKaitholia KamleshShukla Deepali - remains have been found in Africa, Eurasia, and Southeast Asia, with a fossil record dating back to 2 million years, holding a significant place in human evolution. However, due to limited molecular evidence, its genetic characteristics, diversity, and potential connections to other archaic homonins and modern humans have long remained unresolved. To address these issues, a research team led by Qiaomei Fu from the Institute of Vertebrate Paleontology and Paleoanthropology of the Chinese Academy of Sciences, in collaboration with other archaeological institutions, successfully extracted ancient enamel proteins from six Middle Pleistocene teeth (~0.4 Ma) from the Zhoukoudian, Hexian, and Sunjiadong sites. Further in-depth paleoproteomic analyses revealed the following breakthroughs. First, a quantitative sex determination pipeline was established based on male-specific amelogenin, Y isoform (AMELY), confirming that five of these specimens are male and one is female. Second, two genetically specific amino acid variants in ameloblastin (AMBN) were identified in all specimens from three sites. One is a newly discovered variant, AMBN-253G, which has not been found in any other archaic or modern human populations. It represents a molecular marker specific to the East Asian Middle Pleistocene lineage, providing solid evidence that specimens from Zhoukoudian, Hexian, and Sunjiadong sites belonged to the same evolutionary lineage. The other variant, AMBN-273V, has previously been identified in Denisovans. However, genetic analysis in this study reveals that this variant may have been introduced into Denisovans through populations related to these Middle Pleistocene , and some of which subsequently contributed to certain modern human populations from Southeast Asia or Oceania. This study obtains lineage-specific molecular information from fossils for the first time, and reshapes our understanding of hominin evolution and the history of genetic admixture in East Asia. - Source: PubMed
Zou Zi-YiRao Hui-YunFu Qiao-Mei - To investigate the abnormal genotyping and its causes at the locus in male samples. - Source: PubMed
Liu ZhenpingYe ZhihuaTong JijunSong JiahuiWu WeiweiHao HongleiFu YanfangZhai Xiandun