ACTRT2
- Known as:
- ACTRT2
- Catalog number:
- 001111A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ACTRT2
Related genes to: ACTRT2
- Gene:
- ACTRT2 NIH gene
- Name:
- actin related protein T2
- Previous symbol:
- -
- Synonyms:
- Arp-T2, ARPM2, FLJ25424
- Chromosome:
- 1p36.32
- Locus Type:
- gene with protein product
- Date approved:
- 2005-11-22
- Date modifiied:
- 2016-10-05
Related products to: ACTRT2
Related articles to: ACTRT2
- Actin-related protein T3 (ACTRT3) is localized in the perinuclear theca (PT) of murine spermatids. We generated Actrt3-/- male mice and showed that they are subfertile, with defects of the acrosome first observed during cap phase. Actrt3 deficiency causes reduced protein levels of the trans-Golgi network markers TGN46 and GOPC and mislocalization of the cis-Golgi protein GM130. Reduction of the autophagy markers LC3B, CTSB and mTOR indicates that loss of ACTRT3 leads to impaired Golgi trafficking and autophagic flux, which are required for acrosome biogenesis. In addition, levels of PFN3, a protein involved in acrosome biogenesis, were significantly reduced. Further, co-immunoprecipitation revealed interaction of ACTRT3 with the PT proteins ACTRT1, ACTRT2, ACTL7A, SPEM2 and the sperm surface protein ZPBP. This suggested that ACTRT3 is a part of the complex 3D scaffold of the PT and contributes to ZPBP localization. Mass spectrometry revealed enrichment of cytoskeletal regulators such as CFL1 and CNN1. Expression of Actrt3 caused changes in HEK239T cell shape and F-actin filament distribution, suggesting a role in cytoskeletal shaping. We conclude that lack of ACTRT3 affects acrosome biogenesis, PT structure and actin remodeling. - Source: PubMed
Publication date: 2026/02/11
Kovacevic AndjelaOrdziniak EvaUmer NailaArevalo LenaHinterlang Leo DZiaeipour SanazSuvilla SaraMerges Gina ESchorle Hubert - Liquid biopsy for detection of circulating tumor DNA (ctDNA) offers a promising, non-invasive strategy for real-time monitoring of cancer. We aimed to develop a universal and sensitive one-tube droplet digital PCR (ddPCR) multiplex assay able to detect ctDNA in plasma from metastatic prostate cancer (PCa) patients. - Source: PubMed
Publication date: 2026/01/29
Kahns SørenZedan Ahmed HAagaard Mads MCanto Luisa M DKjær-Frifeldt SanneEriksen Stine VMadsen Christine VMadsen Jonna SOsther Palle J SHansen Torben FAndersen Rikke F - The death of spermatogonia leads to decreased spermatogenesis and male infertility. Spermatogonia are vulnerable to various external damaging factors, which can cause cell death. However, the mechanism is still unclear. In this study, we found that the actin-related protein T2 (ACTRT2) is specifically expressed in testicular tissue and is associated with spermatogenesis. In vitro, when GC-1 cells (spermatogonial cell line) were treated with busulfan, the proportion of cell death in the low-ACTRT2 group increased significantly. Reactive oxygen species accumulation and typical mitochondrial changes associated with ferroptosis occurred. In vivo, the seminiferous tubules in ACTRT2-/- mice were significantly shrunken. In addition, after being treated with busulfan, spermatogenesis in ACTRT2+/- mice decreased significantly compared to that in wild-type mice. In ACTRT2+/- testes, the expression levels of acyl-CoA synthetase long-chain family member 4 and arachidonic acid 15-lipoxygenase-1 (ALOX15) were upregulated, while the expression levels of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) were downregulated. Finally, we found that the expression of solute carrier family 11 member 2 (SLC11A2), iron responsive element binding protein 2 (IREB2), and transferrin receptor protein 1 (TFRC) increased significantly in the low-ACTRT2 group, which transports iron into the cell to increase the intracellular unstable iron pool. In conclusion, ACTRT2 deficiency leads to intracellular iron overload and damage to mitochondria, ultimately increasing spermatogonia vulnerability to ferroptosis. - Source: PubMed
Chen HaichengLi YanqingLuo DaoshengXie YunLv LinyanYao JiahuiMa MenghuiLiang XiaoyanZhang MinSun XiangzhouZou XuenongDeng ChunhuaYang XingLiu Guihua - Could actin-related protein T1 (ACTRT1) deficiency be a potential pathogenic factor of human male infertility? - Source: PubMed
Zhang QiJin HuijuanLong ShunhuaTang XiangrongLi JiaxunLiu WeiweiHan WeiLiao HaiyuanFu TaoHuang GuoningChen SurenLin Tingting - HIV infection continues to be a major global public health issue. The population heterogeneity in susceptibility or resistance to HIV-1 and progression upon infection is attributable to, among other factors, host genetic variation. Therefore, identifying population-specific variation and genetic modifiers of HIV infectivity can catapult the invention of effective strategies against HIV-1 in African populations. Here, we investigated whole genome sequences of 390 unrelated HIV-positive and -negative individuals from Botswana. We report 27.7 million single nucleotide variations (SNVs) in the complete genomes of Botswana nationals, of which 2.8 million were missing in public databases. Our population structure analysis revealed a largely homogenous structure in the Botswana population. Admixture analysis showed elevated components shared between the Botswana population and the Niger-Congo (65.9%), Khoe-San (32.9%), and Europeans (1.1%) ancestries in the population of Botswana. Statistical significance of the mutational burden of deleterious and loss-of-function variants per gene against a null model was estimated. The most deleterious variants were enriched in five genes: (the Actin Related Protein T2), (homeobox D12), (ATP binding cassette subfamily B member 5), (ATPase phospholipid transporting 8B4) and (ATP Binding Cassette Subfamily C Member 12). These genes are enriched in the glycolysis and gluconeogenesis ( < 2.84e-6) pathways and therefore, may contribute to the emerging field of immunometabolism in which therapy against HIV-1 infection is being evaluated. Published transcriptomic evidence supports the role of the glycolysis/gluconeogenesis pathways in the regulation of susceptibility to HIV, and that cumulative effects of genetic modifiers in glycolysis/gluconeogenesis pathways may potentially have effects on the expression and clinical variability of HIV-1. Identified genes and pathways provide novel avenues for other interventions, with the potential for informing the design of new therapeutics. - Source: PubMed
Publication date: 2023/12/20
Thami Prisca KChoga Wonderful TDandara ColletO'Brien Stephen JEssex MyronGaseitsiwe SimaniChimusa Emile R