ACCN4
- Known as:
- ACCN4
- Catalog number:
- 000997A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ACCN4
Related genes to: ACCN4
- Gene:
- ASIC4 NIH gene
- Name:
- acid sensing ion channel subunit family member 4
- Previous symbol:
- ACCN4
- Synonyms:
- BNAC4
- Chromosome:
- 2q35
- Locus Type:
- gene with protein product
- Date approved:
- 2003-06-05
- Date modifiied:
- 2016-10-05
Related products to: ACCN4
Related articles to: ACCN4
- Analysis of body fluids obtained from crime scenes provides important information for directing criminal investigations. Generally, chemical and immunological techniques, along with molecular methods that utilize RNA, are used for body fluid identification. However, these methods have some limitations, such as handling difficulty, sample consumption, false-positive or -negative results, and sample instability. To address these problems, several researchers have utilized DNA methylation for body fluid identification, including microarrays, combined bisulfite restriction analysis, single-base extension, and pyrosequencing. Although these methods have the advantage of simultaneously analyzing multiple target sites at the same time, they require considerable time and cost. In this study, a methylation-specific PCR (MSP)-based method was used to simultaneously discriminate between three human body fluids (semen, saliva, and blood). We designed primers with different amplicon sizes for each specific CpG region in the three body fluids (ASIC4 for semen, FAM43A for saliva, and FOXO3 for blood). For the accurate analysis, methylation and unmethylation primers were used separately for different amplifications. This MSP-based method can discriminate body fluids in unknown samples, although caution is required when analyzing mixed or low-quantity DNA samples. Our multiplex MSP-based approach enables confirmation of human body fluids and may provide a potentially simpler workflow compatible with conventional capillary electrophoresis platforms compared with methods such as the SNaPshot assay. We anticipate that this multiplex PCR system will be helpful in criminal investigations. - Source: PubMed
Publication date: 2026/04/21
Yu Kyeong-MinAhn Eu-ReePark Myung JinPark Hyun-Chul - BACKGROUND: Immune evasion is a major cause of the poor efficacy of therapies for pancreatic ductal adenocarcinoma (PDAC). However, the underlying mechanism by which MHC-I downregulation leads to low infiltration of cytotoxic T lymphocytes in PDAC remains incompletely elucidated. This study aims to identify the molecules responsible for the low expression of MHC-I and potential novel therapeutic targets, thereby providing a theoretical foundation for reversing immune evasion in pancreatic cancer. METHODS: Bioinformatic analysis identified the acid-sensing ion channel (ASIC4) as a key factor associated with low cytotoxic T lymphocyte (CTL) infiltration in pancreatic ductal adenocarcinoma (PDAC). The correlation between ASIC4 expression and patient prognosis was analyzed via immunohistochemistry (IHC). To elucidate the molecular mechanism by which ASIC4 mediates immune evasion in PDAC, we comprehensively utilized Western blotting, co-immunoprecipitation, and immunofluorescence. An orthotopic PDAC mouse model was established to assess the impact of ASIC4 deletion on CD8⁺ T cell infiltration and tumor growth in vivo. RESULTS: The study found that in pancreatic ductal adenocarcinoma (PDAC), the expression of ASIC4 was significantly upregulated and negatively correlated with the low expression of Major Histocompatibility Complex class I (MHC-I), which was associated with poor patient prognosis. Notably, knocking down ASIC4 led to a significant increase in CD8+ T cell infiltration and slowed tumor growth in vivo. Mechanistic investigations revealed that ASIC4 knockdown restored total and surface levels of MHC-I by inhibiting its autophagic-lysosomal degradation. CONCLUSION: This study revealed that ASIC4 is highly expressed in PDAC, and its elevated expression is significantly associated with poor prognosis in pancreatic cancer patients. Further mechanistic investigations demonstrated that ASIC4 promotes the degradation of MHC-I via the lysosomal pathway. The subsequent reduction in MHC-I expression leads to decreased infiltration of cytotoxic T lymphocytes (CTLs), ultimately accelerating pancreatic cancer progression. Silencing ASIC1 restored MHC-I expression and enhanced the antitumor efficacy of CD8⁺ T cells. These findings identify ASIC4 as a potential therapeutic target and provide a theoretical foundation for reversing the immune-cold phenotype of PDAC and developing combined immunotherapy strategies. - Source: PubMed
Publication date: 2026/02/24
An QiAn ZhongwuBo WeiboYan ChaochunSun NanMa JianxinLi JianyeWang Wenjuan - Menopausal syndrome (MPS) is a symptom of physical and psychosomatic abnormalities that women may face around the time of menopause. Hot flashes are the main symptom. Paeonia lactiflora extract (PLE) is the active ingredient extracted from . It can be used to treat MPS, such as hot flashes. However, its pharmacologic mechanism is unclear. - Source: PubMed
Publication date: 2025/07/22
Cui WeilinSong TingtingGao DongmeiWang XiaoyuSun YaFu LiyuHan YichaoWang Jieqiong - Lower-grade glioma (LGG) is a molecularly heterogeneous tumor in which acidic microenvironments and immune interactions critically influence prognosis. Acid-sensing ion channels (ASICs), particularly ASIC1, remain understudied in glioma biology despite their pH-sensing roles. This study investigates ASIC1/ASIC4 in LGG prognosis, immune regulation, and molecular subtyping. - Source: PubMed
Publication date: 2025/07/25
Mao KeleiGuo AngyangLi YizeYang WendiXiong YiduoWang WeiYan Xiaodong - Ion channels play a crucial role in various physiological processes, yet their functions in the reproductive system remain underexplored. This study investigates the expression and the localization of ASIC2, ASIC4, and PIEZO2 ion channels in the reproductive tracts of prepubertal bitches. Western blotting on samples from eight prepubertal bitches confirmed the presence of these ion channels in ovarian, uterine, and uterine tubes tissues, and validated antibody specificity. Immunohistochemistry revealed that all primordial follicles expressed these ion channels, while only some developing follicles showed immunolabeling. These findings suggest ion channels' potential involvement in oocyte differentiation and maturation. The localization of these channels in uterine tubes, uterine lining, and glandular epithelium suggests a role in tissue maintenance, oocyte transport, and embryo implantation. Additionally, their expression in the tunica media of reproductive vasculature points to a potential role in vascular regulation. Future studies are needed to elucidate the specific mechanisms underlying the role of these channels in reproductive physiology. - Source: PubMed
Publication date: 2025/05/05
Mhalhel KamelCavallaro MauroPansera LidiaFranco Gianluca AntonioMontalbano GiuseppeLaurà RosariaAbbate FrancescoGermanà AntoninoLevanti MariaAragona Marialuisa