ACAD11
- Known as:
- ACAD11
- Catalog number:
- 000977A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ACAD11
Related genes to: ACAD11
- Gene:
- ACAD11 NIH gene
- Name:
- acyl-CoA dehydrogenase family member 11
- Previous symbol:
- -
- Synonyms:
- FLJ12592
- Chromosome:
- 3q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2006-01-09
- Date modifiied:
- 2019-03-21
Related products to: ACAD11
Related articles to: ACAD11
- Recent studies have shown that fractionated feeding can promote the development of hyperphagic behavior in mule ducks. In parallel, embryonic thermal programming (TM) has been demonstrated to enhance hepatic energy storage under force-feeding conditions. In this exploratory study, we evaluated the combined effects of fractionated feeding and embryonic TM on short-term hepatic lipid accumulation in ducks. Two groups were compared: a CONTROL group (37.6°C during all incubation period) and a TM group (39.3°C for 16 hours per day from embryonic days 11 to 21). TM increased the embryonic relative expression of stress-response genes (HSP90, HSP10, HSF5) compared to controls. At 7 weeks of age, the animals underwent a 5-day acclimation period during which they had access to feed for only 1 hour per day. After this acclimation phase, ducks were offered three voluntary 1-hour meals, spaced 12 hours apart, with a commercial growth diet. Liver weight, hepatic energy composition (glycogen and lipids), and expression of genes involved in hepatic metabolism were assessed before the first meal, 6 hours after each meal, and 24 hours after the last meal. Feed intake remained high during the first two meals but decreased during the third. Despite this, liver weight and energy content increased up to 6 hours after the third meal, peaking in total lipids and oleate proportion, accompanied by upregulation of lipogenic genes expressions (ACC, ACLY, FASN, SCD1, ELOVL6) and downregulation of oxidative genes expressions (ACOX1, CPT1a, ACSL1, ACAD11). Early activation of lipogenic gene expression in the TM group led to higher liver weight and lipid content after the first meal. However, this reflected a temporal shift in lipid synthesis rather than increased fattening, as no differences were observed at the end of the protocol. Overall, embryonic thermal programming modulates the timing of hepatic lipid metabolism in ducks under voluntary fractionated feeding. This exploratory approach requires further optimization, particularly to prolong the transient hyperphagia observed. Nevertheless, it provides a first step toward understanding how nutritional strategies combined with embryonic programming may influence hepatic lipid deposition. - Source: PubMed
Publication date: 2026/05/07
Zwick Laura-LouHuot JoséphineHeraud CécileLasserre MarieSurget AnneGontier KarineRoy JérômePanserat StéphaneHoussier Marianne - Fat deposition plays a crucial role in regulating the production performance and meat quality of broilers. Although the heterogeneity of mammalian adipocytes has been extensively studied, research on the molecular mechanisms underlying differences in lipid droplet accumulation in avian adipocytes remains limited. This study confirmed a significant positive correlation (R > 0.81, < 0.001) between the SSC signal and lipid droplet content via fluorescence staining of lipid droplets, Oil Red O staining, and triglyceride (TG) quantification. Based on this, a label-free sorting strategy using SSC signals was established to sort differentiated chicken preadipocytes, obtaining high lipid droplet (H) and low lipid droplet (L) subpopulations, which were subsequently subjected to transcriptome sequencing and differential gene expression (DEG) analysis, followed by GO and KEGG enrichment analysis. The results indicated no significant differences in the expression of adipogenesis marker genes (, , , , ) between the high lipid droplet (H) and low lipid droplet (L) groups, suggesting that both groups are at similar stages of differentiation. KEGG analysis revealed that both the H vs. NC and L vs. NC comparisons were enriched in common pathways, including the PPAR signaling pathway, ECM-receptor interaction, focal adhesion, cytokine-receptor interaction, and calcium-Apelin signaling pathway, suggesting that both groups of cells had activated the adipogenesis program. GO analysis showed that, in both H vs. NC and L vs. NC comparisons, differentially expressed genes (DEGs) were enriched in biological processes (BPs) related to cell adhesion, nucleosome assembly, chromatin remodeling, and receptor activity, as well as cellular components (CCs) such as the extracellular matrix, cytoskeleton, and nucleosome organization, indicating extensive gene reprogramming and activation of signaling transduction during differentiation. In the H vs. L comparison, enriched pathways included ABC transporters, ECM-receptor interaction, focal adhesion, gap junctions, microtubule-related processes, and neuroactive ligand-receptor interactions, involving lipid transmembrane transport, cytoskeleton stabilization, and signal transduction regulation, suggesting that high lipid droplet cells are more mature in lipid droplet transport, storage, and homeostasis maintenance. GO enrichment results further supported this conclusion, as H vs. L specifically enriched processes related to microtubule-related processes, cell cycle, and redox reactions (BPs), as well as chromosome organization, cytoskeleton, and motor activity (CC/MF), indicating that high lipid droplet cells maintain lipid droplet fusion and metabolic homeostasis via enhanced microtubule transport and antioxidant regulation. Differential gene analysis revealed that the L group upregulated genes associated with fatty acid synthesis and elongation (, , , , ), cholesterol and isoprenoid biosynthesis (, , , , , , ), and fatty acid oxidation (, , , ), reflecting a metabolic characteristic of concurrent lipid synthesis and mobilization; the H group, conversely, upregulated genes associated with lipid droplet formation and storage (, , , , ), lipid transport (, , , , ), and antioxidant defense (, , ), exhibiting a storage and homeostasis-oriented metabolic state. In the NC, L, and H groups, the expression of five genes-, , , , and -showed a gradual increase, suggesting that these genes were associated with preadipocyte differentiation and lipid droplet deposition. In summary, although the high and low lipid droplet subpopulations of chicken preadipocytes exhibit similar differentiation states, they form distinct metabolic orientations. The L group is characterized by active lipid synthesis, fatty acid oxidation, and membrane lipid remodeling, while the H group predominantly features lipid droplet storage, lipid transport, and antioxidant homeostasis. This study highlights the molecular mechanisms underlying the metabolic heterogeneity of avian adipocytes and provides a theoretical basis for poultry fat deposition regulation and genetic improvement. - Source: PubMed
Publication date: 2026/03/12
Wang BoyuLi YantaoWang YakeChen JiayiWang JialiLi XiaopingLi Zhenhui - The incidence of clear cell renal cell carcinoma (ccRCC) is increasing every year. Mitophagy is a unique form of autophagy that plays a crucial role in cancer development and invasion. However, its role in ccRCC remains to be fully elucidated. - Source: PubMed
Publication date: 2025/12/18
Jiang ZhongjunWang LanlanHe ZhongrunGuo LianLuo WenFu YingXiao QiyuChen GuanglanLiu Yinzi - The obligate intracellular Gram-negative bacterium Chlamydia psittaci, a zoonotic pathogen transmissible between birds and humans, has played a pioneering role in research on its membrane-bound replicative niche termed the inclusion. Inclusion membrane proteins (Inc proteins) are crucial for Chlamydia-host interactions and were first identified in C. psittaci. This study investigates putative C. psittaci Inc proteins by a combination of in silico analyses, immunofluorescence and, strikingly, a new Inc/GFP chimera protein-based interactomics approach to identify host cellular interaction partners. Here, we report a novel C. psittaci Inc protein, Cps0558, along with respective host cellular interaction partners, in particular ACAD11, which is involved in lipid metabolism. We confirm their physical interaction in the native infection context, supporting the physiological relevance of our chimera-based screen. Furthermore, new interaction partners for the known Inc protein IncA are identified, revealing a potential role of IncA as modulator of the host ubiquitylation system. These results provide further insights into the biology of C. psittaci and present a novel tool for studying Inc proteins under conditions closely resembling their natural tertiary structure. - Source: PubMed
Gensch Jean-MarcScholz JanaIngmundson AlyssaRose LauraDoellinger JoergBanhart SebastianHeuer Dagmar - Crystallin proteins serve as both essential structural and as well as protective components of the ocular lens and are required for the transparency and light refraction properties of the organ. The mouse lens crystallin proteome is represented by αA-, αB-, βA1-, βA2-, βA3-, βA4-, βB1-, βB2-, βB3-, γA-, γB-, γC-, γD-, γE, γF-, γN-, and γS-crystallin proteins encoded by 16 genes. Their mutations are responsible for lens opacification and early onset cataract formation. While many cataract-causing missense and nonsense mutations are known for these genes, including the human CRYBB3 gene, the mammalian loss-of function model of Crybb3 remains to be established. Herein, we generated the first mouse model via deletion of the Crybb3 promoter that nearly abolished expression of the βB3-crystallin. Histological analysis of lens morphology using newborn βB3-crystallin-deficient lenses revealed disrupted lens morphology with early-onset phenotypic variability. In-depth lens proteomics at four time points (newborn, 3-weeks, 6-weeks, and 3-months) showed both down- and up-regulation of various proteins, with the highest divergence from control mice observed in 3-months lenses. Apart from the βB3-crystallin, Smarcc1/Baf155 was down-regulated in all four stages. In addition, downregulation of Hspe1, Pdlim1, Ast/Got, Lsm7, Ddx23, and Acad11 was found in three time points. Finally, we show that the βB3-crystallin promoter region, which contains multiple binding sites for the transcription factors AP-2α, c-Jun, c-Maf, Etv5, and Pax6 is activated by FGF2 in primary lens cell culture experiments. Together, these studies establish the mouse Crybb3 loss-of-function model and its disrupted crystallin and non-crystallin proteomes. - Source: PubMed
Publication date: 2025/08/19
Rayêe DanielleWilmarth Phillip AVanSlyke Judy KZientek KeithReddy Ashok PMusil Linda SDavid Larry LCvekl Aleš