ABTB1
- Known as:
- ABTB1
- Catalog number:
- 000968A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ABTB1
Related genes to: ABTB1
- Gene:
- ABTB1 NIH gene
- Name:
- ankyrin repeat and BTB domain containing 1
- Previous symbol:
- -
- Synonyms:
- BPOZ, EF1ABP, Btb3, BTBD21
- Chromosome:
- 3q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-03-14
- Date modifiied:
- 2016-10-05
Related products to: ABTB1
Related articles to: ABTB1
- BACKGROUND: Icotinib is a first-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) widely used in the treatment of EGFR-mutant lung adenocarcinoma (LUAD). However, heterogeneous responses and the development of resistance limit durable clinical benefit. The molecular regulators that modulate EGFR signaling output and influence cellular sensitivity to icotinib remain incompletely defined. METHODS: An integrative discovery framework was implemented by combining genome-wide CRISPR–Cas9 functional screening under icotinib selection pressure with RNA sequencing analyses performed under basal and icotinib-treated conditions. Candidate regulators were prioritized through multi-layer integration of functional dependency, transcriptional response, and disease-context expression data. Clinical associations were evaluated using public LUAD datasets. Functional validation was performed using cell proliferation assays, dose–response analyses, and biochemical approaches. Protein interaction and signaling mechanisms were examined using immunoprecipitation–mass spectrometry, co-immunoprecipitation, immunofluorescence, and molecular docking analyses. RESULTS: Integrated functional and transcriptomic analyses identified ABTB1 as a candidate regulator associated with icotinib response. ABTB1 expression was reduced in LUAD and correlated with aggressive tumor features and unfavorable overall survival. Functionally, ABTB1 overexpression enhanced cellular sensitivity to icotinib, as evidenced by suppressed proliferation, leftward shifts of dose–response curves, and reduced IC50 values. Proteomic profiling identified EGFR as a prominent ABTB1-associated protein, which was further validated by biochemical and spatial co-localization analyses. Mechanistically, ABTB1 overexpression attenuated EGFR phosphorylation and downstream STAT3 and NF-κB signaling, accompanied by coordinated transcriptional remodeling of gene programs related to stress response, inflammatory signaling, and cell fate regulation. CONCLUSIONS: This study identifies ABTB1 as a non-mutational regulator of EGFR signaling that modulates cellular sensitivity to icotinib in LUAD. By integrating functional screening, transcriptomic network analysis, and mechanistic validation, our findings reveal a receptor-proximal control layer shaping icotinib response and highlight ABTB1 as a potential biomarker and modulatory factor in icotinib-treated LUAD. - Source: PubMed
Publication date: 2026/04/27
Li Si-YangXia LuXu PengLi Gen-HongYang Bing-ChunWu Jing-XunZhang Fu-XingLian Jia-Bian - Emerging evidence has implicated the multifaceted roles of TRIM proteins in glioblastoma; however, the function of TRIM4 in glioblastoma pathobiology has not been clarified. - Source: PubMed
Publication date: 2026/04/23
Liu GexiZhang QinghaoZhong JiachengLiu YujieChen JieFeng TianxiangZhou JingjieSun XiaochuanCui HongjuanShi Shuang - Child maltreatment (CM) covers various forms of physical, emotional, and sexual abuse and neglect. Although the scientific literature has extensively documented that exposure to sexual abuse and/or neglect during childhood can cause long-term harm to an individual's overall well-being, the psycho-biological impact of these specific forms of CM requires further exploration. This pilot study tested the hypothesis that experiencing childhood neglect and sexual abuse are associated with psychological alterations as well as biological alterations, namely blood gene expression changes. This study encompasses a group of volunteer university students, who completed a battery of questionnaires to evaluate the presence of neglect and sexual abuse experience during childhood (CTQ-SF) and psychological distress (SCL-90-R; BDI-II). Both subsets were compared with control groups. Peripheral blood mononuclear cells were collected from all groups to extract RNA and perform genome wide expression analyses. Neglected and sexually abused individuals showed evidence of biological alterations. Through a genome wide transcriptomic analysis, combined with multivariate nomogram analysis, we identified two groups of 5 genes, the changes in expression of each group identified a subject who experienced either neglect or sexual abuse, with a probability of 95%. Among the first group of genes, the expression of correlated significantly with depressive scores in neglected individuals. Among the second group of genes, the expression of correlated significantly with general psychological distress in sexually abused individuals. These results support that childhood neglect and sexual abuse are associated with gene expression changes and psychological outcomes, underscoring the importance of refining the diagnostic process with more objective screening and assessment tools. - Source: PubMed
Publication date: 2025/11/06
Pesca ChiaraLo Iacono LuisaBussone SilviaComincini SergioTrentini CristinaCarola Valeria - [This retracts the article DOI: 10.1155/2022/8131531.]. - Source: PubMed
Publication date: 2024/03/20
International BioMed Research - Colorectal cancer (CRC) is a prominent form of cancer globally, ranking second in terms of prevalence and serving as a leading cause of cancer-related deaths, but the underlying biological interpretation remains largely unknown. We used the summary data-based Mendelian randomization method to integrate CRC genome-wide association studies (n = 7062; n = 195,745) and expression quantitative trait summary data in peripheral whole blood (Consortium for Architecture of Gene Expression: n = 2765; Genotype-Tissue Expression [v8]: n = 755) and colon tissue (colon-transverse: n = 406; colon-sigmoid: n = 373) and identified related genes. Genes , , , and have emerged as significant prognostic markers for CRC patient survival. Functional analysis revealed their involvement in cancer cell migration and invasion mechanisms, providing valuable insights for the development of future anti-CRC drugs. We successfully identified five CRC risk genes, providing new insights and research directions for the effective mechanisms of CRC. - Source: PubMed
Publication date: 2024/02/21
Zhang CuizhenHuang WenjieNiu WanjieYang HuiyingZheng YingyiGao XuanQiu Xiaoyan