ABI3
- Known as:
- ABI3
- Catalog number:
- 000956A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ABI3
Related genes to: ABI3
- Gene:
- ABI3 NIH gene
- Name:
- ABI family member 3
- Previous symbol:
- -
- Synonyms:
- NESH, SSH3BP3
- Chromosome:
- 17q21.32
- Locus Type:
- gene with protein product
- Date approved:
- 2004-03-11
- Date modifiied:
- 2016-10-05
Related products to: ABI3
Related articles to: ABI3
- Pentatricopeptide repeat (PPR) proteins are key regulators of the organellar RNA metabolism in plants. However, the functions of mitochondrial PPR proteins belonging to the subclass of P-type PPR factors containing the SMR domain remain much less understood. Here, we characterize the EMBRYO DEFECTIVE 2217 (EMB2217/At1g79490), an essential PPR-SMR factor in Arabidopsis thaliana. T-DNA insertional lines at the AT1G79490 gene-locus exhibit embryonic arrest at the late heart stage and display defective germination and seedling establishment. Partial complementation using an ABI3 promoter-driven strategy enables efficient germination and the rescue of homozygous emb2217 plantlets. The pABI3::EMB2217 emb2217 -/- seedlings display severe growth defects due to impaired mitochondrial function, tightly associated with impaired OXPHOS activity. Analyses of mitochondrial RNA profiles reveal that EMB2217 is required for the processing of multiple group II introns that reside in the coding regions of several complex I (CI) subunits, the cox2 subunit of CIV, and the ribosomal rps3 factors. Our data further show that RNA maturation defects induce alternative electron transport and stress-response pathways, which are associated with developmental defects and modulation of photosynthetic and cellular metabolic processes. Together, we identify EMB2217 as a general mitochondrial splicing factor whose loss compromises OXPHOS biogenesis and function, cellular energy supply, and plant development. - Source: PubMed
Marchetti FernandaBalestieri NehuenBecerra-Agudelo EvelynValiñas MatíasAmigo NataliaMatan RoeiMizrahi RonTakenaka MizukiWelchen ElinaPagnussat Gabriela CarolinaOstersetzer-Biran OrenZabaleta Eduardo - Gibberellins (GAs) are central regulators of plant growth and development, yet their involvement in somatic embryogenesis (SE) has received limited attention and often produces contradictory results. This review critically examines the current knowledge on the role of GAs in SE by integrating evidence from physiological, molecular, and developmental studies across a wide range of plant species. Available data indicate that exogenous GAs and GA biosynthesis inhibitors can either promote or inhibit the induction, progression, and conversion of somatic embryos, depending on the species, genotype, explant origin, developmental stage, and culture conditions. These opposing effects underscore that exogenous responses are tightly linked to endogenous GA homeostasis. Particular attention is paid to GA metabolism and signaling genes, whose expression patterns during SE reveal substantial interspecific and genotypic variation. Endogenous GA profiles reveal that both high and low levels of bioactive GAs correlate with SE induction across different systems, suggesting that endogenous GA levels may be a key determinant of embryogenic competence and may underlie major differences in regeneration capacity among species and genotypes. Furthermore, GAs function within a broader regulatory network through extensive crosstalk with other hormones (auxins, abscisic acid, cytokinins, and ethylene), environmental factors (e.g., light and temperature) and interactions with major embryogenesis-related transcription factors such as LEC1, LEC2, FUS3, ABI3, AGL15, AGL18, and BBM. These interactions position GAs as dynamic components of a multilayered hormonal and transcriptional framework that controls the switch from somatic to embryogenic development. Integrative approaches-including hormone profiling, gene expression analysis, functional genetics, and developmental studies-are essential to elucidate the precise function of GAs in SE and thereby improve protocols for plant regeneration. - Source: PubMed
Publication date: 2026/07/27
Belić MajaZdravković-Korać SnežanaMilojević Jelena - A total of 164 APETALA2/ethylene-responsive factor (AP2/ERF) genes were identified in Hydrangea macrophylla, and HmaERF87 positively contributes to leaf spot resistance. The APETALA2/ethylene-responsive factor (AP2/ERF) transcription factor family plays important roles in plant stress responses, but its contribution to disease resistance in Hydrangea macrophylla (hydrangea) remains poorly understood. In this study, 164 AP2/ERF genes were identified in the H. macrophylla genome and classified into APETALA2 (AP2), ethylene-responsive factor (ERF), dehydration-responsive element-binding (DREB), and related to ABI3/VP1 (RAV) subfamilies. Their chromosomal distribution, conserved motifs, gene structures, and duplication patterns were analyzed. A total of 46 pathogen-responsive H. macrophylla AP2/ERF (HmaERF) genes were identified from the RNA sequencing (RNA-seq) dataset of resistant and susceptible cultivar leaves collected before and after Corynespora cassiicola inoculation. Promoter analysis revealed that the HmaERF genes with upregulated expression post-C. cassiicola infection showed a higher frequency and copy number of jasmonate-responsive cis-regulatory elements, suggesting their possible involvement in hormone-mediated defense responses. Three infection-induced candidate genes, including HmaERF56, HmaERF87, and HmaERF129, were selected for functional validation using virus-induced gene silencing (VIGS) in hydrangea leaf discs. Silencing of HmaERF87 expression via VIGS significantly increased lesion development after C. cassiicola inoculation, whereas the transient overexpression of HmaERF87 reduced the area of leaf disc lesions. Subcellular localization showed that the HmaERF87 protein was localized in the nucleus, and yeast assays indicated that its transcriptional activation activity was mainly associated with the C-terminal region of the protein. These results support a role for HmaERF87 as a positive regulator of H. macrophylla resistance to leaf spot disease and provide a candidate gene for further studies of disease resistance in hydrangea. - Source: PubMed
Publication date: 2026/08/07
Chen ShuyuanLiu XintongZhang ChengweiZhang ZiqinChen HuijieHan YongChen ShuangshuangFeng JingWang GuanglingLi ChangWu ZedongDeng Yanming - Airway smooth muscle (ASM) cells are central to bronchoconstriction and airway hyperresponsiveness in asthma, yet a comprehensive view of their transcriptional landscape has been lacking. - Source: PubMed
Publication date: 2026/08/03
Roy SaptarshiMandal Rahul ShubhraKurian SunilSharma Pawan - The Thr445Met substitution in the novel allele med25-5 uncouples seed dormancy from flowering, and MED25 acts as a negative regulator of seed dormancy via the DOG1 and ABA pathways. MED25, a pivotal subunit of the Mediator complex, regulates diverse biological processes, but its role in seed dormancy remains unclear. Here, we identified a novel MED25 allele, med25-5, harboring a Thr445Met substitution in the MD domain, which enhances seed dormancy without affecting flowering time in Arabidopsis thaliana. The med25-5 mutant exhibited increased dormancy with altered expressions in DOG1 and ABA signaling genes (e.g., ABI1, NHL6) and increased sensitivity to ABA and PAC. Either dog1-3 or abi3-10 can completely repress the enhanced seed dormancy of med25, indicating MED25 regulates dormancy requiring the DOG1 and ABA pathways. The Thr445 residue is conserved across crops, highlighting MED25's potential for crop improvement against pre-harvest sprouting. Our study uncovers MED25 as a negative regulator of seed dormancy, providing insights into the molecular mechanisms coordinating dormancy and flowering. - Source: PubMed
Publication date: 2026/07/02
Yang YueLi Xiao-YingDing RunCao HongLiu YongxiuLi Yu