ABCF2
- Known as:
- ABCF2
- Catalog number:
- 000931A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ABCF2
Related genes to: ABCF2
- Gene:
- ABCF2 NIH gene
- Name:
- ATP binding cassette subfamily F member 2
- Previous symbol:
- -
- Synonyms:
- EST133090, ABC28, M-ABC1, HUSSY-18
- Chromosome:
- 7q36.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-10-26
- Date modifiied:
- 2015-11-13
Related products to: ABCF2
Related articles to: ABCF2
- Paralytic shellfish toxins (PSTs) are widely distributed neurotoxins that accumulate and transform in bivalves, but the metabolic mechanisms after exposure of Azumapecten farreri (A. farreri) to PSTs remain unknown. To verify the phase I (cytochrome P450, CYP450)-II (glutathione S-transferase, GST)-III (ABC transport) metabolic genes for PSTs, we used RNA interference (RNAi) to silence target genes (CYP46A1, GSTM1, and ABCF2), measured PSTs content, and performed single nucleotide polymorphism (SNP) screening. Injection of small interfering RNA (siRNA) fragments into the scallop adductor muscle silenced target genes in multiple tissues. The three most effective fragments were selected. Secondary injection of screening fragments prolonged the silencing duration, with a silencing efficiency of 12.0%-100%. Silencing increased PSTs accumulation. Notably, with CYP46A1 silencing, the PSTs accumulation (1016 µg STXeq/kg) reached 3.85 times that of the control group. This silencing also delayed the conversion of highly toxic components (GTX1-4) to components with low toxicity (C1-2), thereby prolonging the PSTs metabolic process. SNP analyses indicated that PSTs exposure increased homozygous mutations in CYP49A1 (511908 site), this site is potentially involved in PSTs metabolism. In summary, this study optimized an RNAi method and confirmed the existence of a three-phase metabolic mechanism in scallops. CYP450 was found to be the main mode of PSTs metabolism. This study provides data regarding PSTs metabolic genes and loci in bivalves, as well as valuable molecular markers for anti-toxin breeding in shellfish. - Source: PubMed
Publication date: 2026/09/02
Lu LiminZheng GuanchaoLin YichenDong ChenfanPeng JixingWu HaiyanTan Zhijun - can cause serious diseases in many animal species and humans, yet the mechanisms of its pathogenesis remain to be elucidated. In this study, we found that the lipoprotein E (PlpE) of contributed to bacterial adhesion to host cells. Through turboID-based proximity labeling screening, we identified ATP-binding cassette sub-family F member 2 (ABCF2) as a host-interaction protein for PlpE, and Asp-123 in PlpE was identified as the crucial amino acid residue for the interaction. Knocking out ABCF2 significantly reduced the adhesion of to host cells. Additionally, we demonstrated that infection upregulated the expression of host ABCF2 by activating the NF-κB signaling. Furthermore, we showed that ABCF2 was involved in the -induced p53-dependent apoptotic signaling pathway. To the best of our knowledge, this is the first identification of ABCF2 as a host factor contributing to the adhesion of and only the second report of ABCF2's involvement in bacterial pathogenesis. - Source: PubMed
Publication date: 2026/07/19
Shang YuyaoWang FeiChen MenghanWang ZihaoLiu HanyuanHua LinChen HuanchunWu BinPeng Zhong - The sustainable development of shrimp aquaculture is significantly compromised by Vibrio parahaemolyticus infections. Identifying host resistance genes and characterizing their immunological roles are essential for developing effective disease control strategies. In this study, we conducted a comparative transcriptomic analysis of intestinal tissues from Penaeus vannamei exhibiting varying degrees of pathological damage post-V. parahaemolyticus challenge to identify key resistance genes. KEGG enrichment analysis revealed that the ABC transporter pathway was markedly enriched among upregulated genes in both the 9 h vs 0 h and 48 h vs 0 h comparison groups. Based on the expression profiles and domain characteristics of genes within this pathway, the full transporter PvABCA3, half transporter PvABCC1, and soluble protein PvABCF2 were selected for RNAi assays. The result indicated that silencing PvABCF2, but not PvABCA3 and PvABCC1, significantly increased mortality, tissue damage, and Vibrio load in V. parahaemolyticus-challenged shrimp. Further investigation revealed that PvABCF2 silencing substantially suppressed the expression of antimicrobial peptides (AMPs), components of the proPO-activating system, and key genes involved in the JAK-STAT and NF-κB signaling pathways. These findings suggested that the increased susceptibility of shrimp to V. parahaemolyticus following PvABCF2 silencing may be associated with downregulation of these specific immune-related genes. Moreover, one SNP within PvABCF2 was found to be markedly associated with resistance to V. parahaemolyticus via SNP association analysis. Collectively, these results suggested that PvABCF2 was involved in defense response against V. parahaemolyticus and identified a potential molecular marker for disease-resistant breeding. - Source: PubMed
Publication date: 2026/07/07
Luo Shuang-ShuangShi Lin-LinLiu Qing-YunChen Xiu-LiWang Ai-JinZhang Yi-QianYang Chun-LingHong-Liu Zhao Yong-ZhenWang Huan-Ling - Nile tilapia (Oreochromis niloticus) is a widely farmed freshwater fish. Feeding with faba bean (Vicia faba L.) for 90-120 days can improve the muscle quality of tilapia. However, the underlying mechanism remain unclear. In the present study, tilapia were fed a faba bean-based diet for 120 days to induce muscle crisped, and ordinary tilapia fed a conventional diet were used as controls. Muscle histological characteristics were evaluated using hematoxylin and eosin staining, and transcriptome sequencing was conducted to explore molecular changes associated with the crisped muscle phenotype. The results showed that, as compared to ordinary tilapia, the fiber diameter and area were significantly reduced in crisped tilapia (p < 0.05), while the muscle fiber density was significantly increased (p < 0.05). In total, 576 differentially expressed genes (DEGs) were identified (FDR < 0.05), of which 211 were significantly up-regulated and 365 significantly down-regulated. Further analysis showed that DEGs associated with myofibroblast proliferation were up-regulated in crisped tilapia, while the glycolytic pathway was inhibited. The expression levels of muscle-related genes (i.e., actc1, myo7a, cib2, abcf2, and pfkfb2) were significantly higher in crisped tilapia than ordinary tilapia (p < 0.05), whereas the expression levels of gapdh, pgam2, eno3, and g6pi were significantly decreased (p < 0.05). Several DEGs and signaling pathways were identified. These findings provide transcriptomic evidence linking dietary faba bean feeding to muscle fiber remodeling and metabolic modulation in tilapia, offering a molecular basis for improving fillet quality through nutritional strategies. - Source: PubMed
Publication date: 2026/01/08
Li QingqingHuang YaoXie XiLiang ShaowenLin Li - Elexacaftor-tezacaftor-ivacaftor (ETI) is a cystic fibrosis transmembrane conductance regulator (CFTR) modulator that improves clinical outcomes in adolescents with cystic fibrosis. We aimed to investigate the effect of ETI on lung structural damage. - Source: PubMed
Publication date: 2025/10/22
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