ABCA8
- Known as:
- ABCA8
- Catalog number:
- 000890A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ABCA8
Related genes to: ABCA8
- Gene:
- ABCA8 NIH gene
- Name:
- ATP binding cassette subfamily A member 8
- Previous symbol:
- -
- Synonyms:
- KIAA0822
- Chromosome:
- 17q24.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-06-11
- Date modifiied:
- 2019-03-21
Related products to: ABCA8
Related articles to: ABCA8
- Cancer-associated fibroblasts (CAFs) serve key roles in gastric cancer progression, however, the role of specific CAF subsets, particularly lipid-rich CAFs (lipo-CAFs), remains unclear. Therefore, the present study aimed to investigate whether lipo-CAFs are associated with gastric cancer progression. A total of 28 lipo-CAF-associated genes was used to classify patients with gastric cancer in The Cancer Genome Atlas Stomach Adenocarcinoma dataset. Differential expression analysis was subsequently performed to identify biological processes and pathways associated with the lipo-CAF phenotype. Validation analyses were conducted using the GSE84437 dataset and expression and survival analyses of the signature genes were performed using data from the Human Protein Atlas database and Tumor-Immune System Interaction Database. Finally, NIH-3T3 cells were transduced with lentiviral vectors to knock down the candidate genes and were co-cultured with mouse gastric cancer MFC cells to evaluate the fibroblast-mediated effects of these genes on gastric cancer cell behavior The majority of lipo-CAF-associated genes were highly expressed in gastric cancer. Patients were stratified into high- and low-risk groups based on gene expression patterns and these groups exhibited differences in prognosis, energy metabolism-associated pathways and immune infiltration. A prognostic model was constructed using these genes and externally validated. Findings indicated that fatty acid-binding protein 4 (FABP4), CD36 and ATP-binding cassette subfamily A member 8 (ABCA8) may exert tumor-promoting roles in gastric cancer. In fibroblast-based co-culture experiments, knockdown of these three genes in NIH-3T3 cells inhibited the proliferation of co-cultured gastric cancer cells. Lipo-CAF-associated genes were found to be coupled with gastric cancer progression and with distinct immune and stromal features of the tumor microenvironment. Among these genes, FABP4, CD36 and ABCA8 demonstrated tumor-promoting effects in fibroblast-based co-culture experiments, highlighting their potential relevance for prognostic stratification and future therapeutic investigation in gastric cancer. - Source: PubMed
Publication date: 2026/07/30
Liao JunqunWu LinZhou LiLing QiaoZhang Piyun - Within the present study, the aim was to investigate the detailed mechanism of forkhead box protein A2 (FOXA2) in anoikis resistance in lung adenocarcinoma (LUAD). The levels of FOXA2 and ATP-binding cassette subfamily A member 8 (ABCA8) were assessed using public databases. The effects of overexpression (oe)-FOXA2, oe-nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) and sh-ABCA8 on NOX4 levels, aggregation, cell viability, apoptosis and apoptosis-related proteins were analyzed using reverse transcription quantitative PCR, microscopy, MTT assays, flow cytometry and western blotting. Chromatin immunoprecipitation and dual-luciferase reporter assays were employed to determine the interaction between FOXA2 and ABCA8. In LUAD, FOXA2 and ABCA8 mRNA levels were downregulated, with lower expression observed in in A549 cells compared with BEAS-2B cells; this pattern was pronounced under suspension culture, where A549 cells showed a further decrease in FOXA2/ABCA8 expression alongside a marked increase in NOX4 expression, compared with adherent culture. Furthermore, oe-ABCA8 inhibited NOX4 expression and reversed the effects of oe-NOX4 on enhancing cell aggregation, promoting cell viability, decreasing apoptosis, suppressing cleaved caspase-3 expression and increasing NOX4 level in suspended A549 cells. Furthermore, FOXA2 bound to the ABCA8 promoter region and oe-FOXA2 reversed the effects of ABCA8 silencing on suspended A549 cells, including enhanced cell aggregation, increased cell viability, reduced apoptosis, suppressed cleaved caspase-3 expression and elevated NOX4 expression. Collectively, FOXA2 enhanced ABCA8 transcription, leading to inhibition of NOX4 expression and subsequently alleviating anoikis resistance in A549 cells. - Source: PubMed
Publication date: 2026/07/22
Hu NinaLi LingHao JianqingLi LifangPan MengmengHe Jia - Benzo[a]pyrene (BaP), an air pollution-related polycyclic aromatic hydrocarbon, may intersect with molecular pathways relevant to atrial fibrillation (AF), but this relationship remains unclear. - Source: PubMed
Publication date: 2026/06/26
Wang QianchenWu JiejieQi ShaoyuZhang JingwenLiu Yang - Osteoporosis is a metabolic bone disease characterized by reduced bone mass and microarchitectural deterioration, with complex involvement of molecular networks and immune-associated transcriptional dysregulation. - Source: PubMed
Publication date: 2026/06/02
Yi WenjingXu WenanYang TaoLyu XiaolinWang ZitingChen LeyiZhang JingyaZhong YimingWang SonglinWu Buling - This study explores the transcriptomic, mutational, and immunogenic characteristics linked to significantly differentially expressed genes (DEGs) in colorectal (COAD), liver (LIHC), lung (LUAD), gastric (STAD), and breast (BRCA) cancers. Applying integrated bioinformatics algorithms, we discovered common upregulated and downregulated hub genes and assessed their prognostic importance, genomic modifications, copy number variations, functional enrichment, and pathway engagement. The persistent overexpression of ANLN and CTHRC1 in five cancer types, along with poor survival outcomes, underscores their suitability for multi-epitope vaccine development, emphasizing their antigenic potential and significance as universal therapeutic targets. Five genes-ABCA8, PDK4, MT1M, TMEM100, and LIFR-exhibited consistent downregulation and demonstrated tumor-suppressive characteristics. Genomic analyses demonstrated elevated mutation frequencies in ABCA8 and LIFR, predominantly C>T transitions that suggest age-related mutational signatures. Copy number alterations confirmed oncogenic amplifications (ANLN and CTHRC1) and tumor suppressor deletions (e.g., ABCA8). Functional enrichment associated differentially expressed genes with mitosis, chromosome segregation, and metabolic pathways. A multi-epitope vaccine targeting ANLN and CTHRC1 has been established leveraging predicted B-cell and T-cell epitopes, β-defensin as an adjuvant, and efficient linkers. Structural validation indicated desirable folding, stability, and solubility. The vaccine exhibited significant MHC binding, accomplishing 99% global population coverage, alongside strong immune simulation findings. Codon optimization and subsequent cloning into the pET28a(+) vector confirmed the preparation for bacterial expression. ANLN and CTHRC1 demonstrate significant targets for universal immunotherapy. The multi-epitope vaccine demonstrates significant efficacy in silico and has the potential to be widely employed as a cancer immunotherapeutic. - Source: PubMed
Publication date: 2026/04/19
Roy Suronjit KumarHasan RubaitBiswas Mohammad ShahangirPodder Munna KumarMoin Abu TayabPatil Rajesh B