ABCA8
- Known as:
- ABCA8
- Catalog number:
- 000890A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- ABCA8
Related genes to: ABCA8
- Gene:
- ABCA8 NIH gene
- Name:
- ATP binding cassette subfamily A member 8
- Previous symbol:
- -
- Synonyms:
- KIAA0822
- Chromosome:
- 17q24.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-06-11
- Date modifiied:
- 2019-03-21
Related products to: ABCA8
Related articles to: ABCA8
- Within the present study, the aim was to investigate the detailed mechanism of forkhead box protein A2 (FOXA2) in anoikis resistance in lung adenocarcinoma (LUAD). The levels of FOXA2 and ATP-binding cassette subfamily A member 8 (ABCA8) were assessed using public databases. The effects of overexpression (oe)-FOXA2, oe-nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) and sh-ABCA8 on NOX4 levels, aggregation, cell viability, apoptosis and apoptosis-related proteins were analyzed using reverse transcription quantitative PCR, microscopy, MTT assays, flow cytometry and western blotting. Chromatin immunoprecipitation and dual-luciferase reporter assays were employed to determine the interaction between FOXA2 and ABCA8. In LUAD, FOXA2 and ABCA8 mRNA levels were downregulated, with lower expression observed in in A549 cells compared with BEAS-2B cells; this pattern was pronounced under suspension culture, where A549 cells showed a further decrease in FOXA2/ABCA8 expression alongside a marked increase in NOX4 expression, compared with adherent culture. Furthermore, oe-ABCA8 inhibited NOX4 expression and reversed the effects of oe-NOX4 on enhancing cell aggregation, promoting cell viability, decreasing apoptosis, suppressing cleaved caspase-3 expression and increasing NOX4 level in suspended A549 cells. Furthermore, FOXA2 bound to the ABCA8 promoter region and oe-FOXA2 reversed the effects of ABCA8 silencing on suspended A549 cells, including enhanced cell aggregation, increased cell viability, reduced apoptosis, suppressed cleaved caspase-3 expression and elevated NOX4 expression. Collectively, FOXA2 enhanced ABCA8 transcription, leading to inhibition of NOX4 expression and subsequently alleviating anoikis resistance in A549 cells. - Source: PubMed
Publication date: 2026/07/22
Hu NinaLi LingHao JianqingLi LifangPan MengmengHe Jia - Benzo[a]pyrene (BaP), an air pollution-related polycyclic aromatic hydrocarbon, may intersect with molecular pathways relevant to atrial fibrillation (AF), but this relationship remains unclear. - Source: PubMed
Publication date: 2026/06/26
Wang QianchenWu JiejieQi ShaoyuZhang JingwenLiu Yang - Osteoporosis is a metabolic bone disease characterized by reduced bone mass and microarchitectural deterioration, with complex involvement of molecular networks and immune-associated transcriptional dysregulation. - Source: PubMed
Publication date: 2026/06/02
Yi WenjingXu WenanYang TaoLyu XiaolinWang ZitingChen LeyiZhang JingyaZhong YimingWang SonglinWu Buling - This study explores the transcriptomic, mutational, and immunogenic characteristics linked to significantly differentially expressed genes (DEGs) in colorectal (COAD), liver (LIHC), lung (LUAD), gastric (STAD), and breast (BRCA) cancers. Applying integrated bioinformatics algorithms, we discovered common upregulated and downregulated hub genes and assessed their prognostic importance, genomic modifications, copy number variations, functional enrichment, and pathway engagement. The persistent overexpression of ANLN and CTHRC1 in five cancer types, along with poor survival outcomes, underscores their suitability for multi-epitope vaccine development, emphasizing their antigenic potential and significance as universal therapeutic targets. Five genes-ABCA8, PDK4, MT1M, TMEM100, and LIFR-exhibited consistent downregulation and demonstrated tumor-suppressive characteristics. Genomic analyses demonstrated elevated mutation frequencies in ABCA8 and LIFR, predominantly C>T transitions that suggest age-related mutational signatures. Copy number alterations confirmed oncogenic amplifications (ANLN and CTHRC1) and tumor suppressor deletions (e.g., ABCA8). Functional enrichment associated differentially expressed genes with mitosis, chromosome segregation, and metabolic pathways. A multi-epitope vaccine targeting ANLN and CTHRC1 has been established leveraging predicted B-cell and T-cell epitopes, β-defensin as an adjuvant, and efficient linkers. Structural validation indicated desirable folding, stability, and solubility. The vaccine exhibited significant MHC binding, accomplishing 99% global population coverage, alongside strong immune simulation findings. Codon optimization and subsequent cloning into the pET28a(+) vector confirmed the preparation for bacterial expression. ANLN and CTHRC1 demonstrate significant targets for universal immunotherapy. The multi-epitope vaccine demonstrates significant efficacy in silico and has the potential to be widely employed as a cancer immunotherapeutic. - Source: PubMed
Publication date: 2026/04/19
Roy Suronjit KumarHasan RubaitBiswas Mohammad ShahangirPodder Munna KumarMoin Abu TayabPatil Rajesh B - Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by the absence of estrogen receptor, progesterone receptor, and HER2 expression, which limits the availability of targeted therapies and results in poor prognosis. Immune checkpoint blockade (ICB) therapies have emerged as promising treatments by enhancing anti-tumor immunity; however, a substantial proportion of patients with TNBC exhibit primary or acquired resistance. This resistance is largely influenced by the tumor microenvironment (TME). This study uses integrated single-cell and spatial transcriptomics to elucidate key cellular mechanisms of resistance, with particular emphasis on lipid-mediated stromal-immune interactions within the TNBC TME. - Source: PubMed
Publication date: 2026/01/27
Qin WeidongLi DanxiZhang JiaweiWang ShuningHou LanZhang CunWang DonghuiZhang Juliang