CaMK2G
- Known as:
- CaMK2G
- Catalog number:
- 000291A
- Product Quantity:
- 250ul
- Category:
- -
- Supplier:
- ABM
- Gene target:
- CaMK2G
Related genes to: CaMK2G
- Gene:
- CAMK2G NIH gene
- Name:
- calcium/calmodulin dependent protein kinase II gamma
- Previous symbol:
- CAMKG
- Synonyms:
- -
- Chromosome:
- 10q22.2
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-24
- Date modifiied:
- 2016-10-05
Related products to: CaMK2G
Related articles to: CaMK2G
- Human neural organoids (NOs) provide a powerful platform for investigating synaptic development and dysfunction during early neurodevelopment. However, methodologies for isolating functional synaptic structures from these models remain limited. Here, we present a differential centrifugation protocol enabling the enrichment of growth cone particles (GCPs) and immature synaptosomes from air-liquid interface cerebral organoids (ALI-COs) at distinct developmental stages (Day 90 and 150). Notably, the method avoids density gradients, requires minimal starting material while maintaining reproducibility across human and murine tissues. Quantitative proteomic profiling revealed significant enrichment of growth cone markers (e.g., GAP43) and classical synaptosomal proteins (e.g., PCLO, BSN, SYN1). Transmission electron microscopy (TEM) confirmed the presence of membrane-enclosed GCPs with fibrous content and mitochondria in Day 90 isolates, and immature synaptosomes containing synaptic vesicles on day 150. Functional viability of both types of synaptic structures was demonstrated through KCl-induced depolarization, which triggered phosphorylation changes in growth cone proteins (GAP43, MARCKS, MARCKSL1), cytoskeletal regulators (DCLK1, SHTN1, MARK4, MAP1B) and protein kinases (CAMK2G, PRKCE) in Day 90 GCPs, as well as classical synaptic vesicle cycle proteins (SYN1, DNM1, RPH3A) at Day 150. Overall, this study establishes a centrifugation-based protocol for isolating growth cones and immature synapses from human organoids, capturing key stages of synaptic development and enabling scalable, patient-compatible models to study synaptic function and dysfunction in neurodevelopmental and neurodegenerative disorders. - Source: PubMed
Øhlenschlæger Marie SCriscuolo LucreziaJensen PiaLloyd-Davies Sánchez Daniel JSutcliffe MagdalenaBhosale SantoshBogetofte HelleTahir MuhammadJakobsen Lene APihl MariaBrewer JonathanSchwämmle VeitPoulsen Frantz RFreude KristineLancaster Madeline ARobinson Phillip JLarsen Martin R - The global burden of heart failure is escalating, marked by persistently rising prevalence, incidence, and mortality. The emerging hypothesis that the gut microbiome, as a modifiable factor, influences HF pathogenesis through immune modulation. - Source: PubMed
Publication date: 2026/01/23
Wei YingJi XuanruiMao YinanLiu YangLi Yue - : Neuropsychiatric disorders such as Alzheimer's disease (AD), bipolar disorder (BD), and depression exhibit shared glutamatergic abnormalities, although their upstream molecular mechanisms remain poorly defined. Magnesium (Mg) serves as a key regulator of N-methyl-D-aspartate (NMDA) receptor function; however, the role of Mg transporters, particularly SLC41A1, has not been systematically investigated. As NMDA receptor dysregulation contributes to emotional and cognitive impairments, elucidating Mg-NMDA signaling may enable the development of novel therapeutic strategies. : We integrated Mendelian randomization, locus colocalization, human brain transcriptomics, functional enrichment, and co-expression analyses to determine whether SLC41A1 functions as a cross-disorder molecular driver. In addition, in vitro electrophysiological experiments using field potential recordings in hippocampal Schaffer-CA1 synapses were conducted to validate its functional role in NMDA receptor-mediated synaptic transmission. : Genetically elevated SLC41A1 expression increased the risk of AD, BD, depression, and alcohol dependence, with strong colocalization analyses supporting shared causal variants. Transcriptomic profiling revealed SLC41A1 upregulation in AD and BD, with enrichment in magnesium transport, mitochondrial function, and synaptic signaling pathways. Co-expression networks across GTEx brain regions demonstrated strong correlations with NMDA-related genes (e.g., , , ). Under NMDAR-selective recording conditions, both imipramine treatment and SLC41A1 knockdown significantly reduced NMDAR-mediated fEPSP amplitudes, supporting a role for SLC41A1 in regulating NMDA receptor-dependent synaptic responses. : This study identifies SLC41A1 as a magnesium-centered, transdiagnostic therapeutic target that links Mg homeostasis to NMDA-dependent synaptic dysfunction. These findings provide a mechanistic foundation for developing SLC41A1-modulating or magnesium-based therapeutic approaches for mood and cognitive disorders. - Source: PubMed
Publication date: 2026/03/09
Chen XinruDeng WenhaoChen XinruiYu Yang - Shenzhu granules (SG) are composed of five traditional Chinese herbal medicines, all of which are frequently used in different kinds of functional foods. This study systematically evaluates the anti-fatigue effects of SG and explores the underlying mechanisms to support its potential as a functional food. The anti-fatigue effects of SG were assessed through a functional anti-fatigue test and measurement of serum oxidative stress markers. Additionally, multi-omics approaches, including metabolomics, transcriptomics, and proteomics were used to investigate the mechanisms underlying the anti-fatigue effects of SG. - Source: PubMed
Publication date: 2026/03/23
Zhang HongmingLi XingxingLi MengGuo WenjunZhang ManqiGao XiaohanLiu JiaxinLin YanlingXie ShengxuZhang DianwenLiu YueXu Yajuan - Alternative polyadenylation (APA) is a crucial post-transcriptional mechanism generating isoform diversity in the nervous system. While genetic variants significantly influence gene expression, the extent to which they regulate 3' UTR usage in the human brain remains underexplored. We aimed to characterize the landscape of allele-specific alternative polyadenylation (asAPA) and investigate its role in neurodevelopmental and neurodegenerative disorders. - Source: PubMed
Publication date: 2026/02/14
Barney Ryan MQuinones-Valdez GiovanniKing Alison JAmoah KofiWang WeijianXiao Xinshu