Custom pre_miR Construction [customer specifies construct]
- Known as:
- Custom pre_miR Construction [customer specifies construct]
- Catalog number:
- CS940MR-1
- Product Quantity:
- 5 ug plasmid
- Category:
- -
- Supplier:
- SBI
- Gene target:
- Custom pre_miR Construction [customer specifies construct]
Related products to: Custom pre_miR Construction [customer specifies construct]
Related articles to: Custom pre_miR Construction [customer specifies construct]
- Children with intellectual disability are more likely to experience chronic and neuropathic pain, which remains frequently under-recognized due to limitations in self-reporting and objective assessment tools. Epigenetic mechanisms, particularly DNA methylation, are believed to influence pain perception. This study investigates the differences in methylation patterns between children with intellectual disability and their age- and sex-matched neurotypical controls. - Source: PubMed
Publication date: 2026/05/18
Celsi FulvioZupin LuisaSenn Francescad'Adamo PioCappellani StefaniaDe Zen LuciaCozzi GiorgioSelicorni AngeloBarbi EgidioPeri Francesca - Hepatitis B virus (HBV) replication is tightly controlled by host stress and innate immune pathways. The small noncoding RNA nc886 (vtRNA2-1) is a known endogenous inhibitor of protein kinase R (PKR), but its role in HBV biology remains unclear. This study aimed to define the function of the nc886-PKR-eIF2α axis in HBV-replicating hepatoma cells and to determine whether nc886 depletion suppresses HBV replication via PKR-dependent translational control. - Source: PubMed
Publication date: 2026/03/31
Piracha Zahra ZahidSaeed Umar - Peripheral artery disease (PAD) is a progressive vascular condition with high risks of limb amputation and adverse clinical outcomes. Despite advancements in revascularization techniques, risk stratification remains limited, especially in predicting long-term limb-specific complications. Epigenetic markers, such as DNA methylation, have emerged as potential tools for improving prognostication in vascular disease. - Source: PubMed
Publication date: 2026/02/27
Tsai Ming-LungHuang Yi-ChunHo Ming-YunYeh Jih-KaiChen Chun-ChiChia Chin-YuanFan Wen-LangHsieh I-ChangTsai Chi-NeuWang Chao-Yung - RNA molecules can undergo modification by N-glycans and be displayed on the cell surface. However, recent studies have focused primarily on N-glycan modifications on small RNAs less than 200 nt in length; the transcriptome-wide subtypes of glycosylated RNAs (glycoRNAs) remain poorly characterized. Since glycoRNAs account for only a fraction of the total transcriptome, a validation system for their accurate analysis has not yet been established. In this study, we aimed to comprehensively characterize transcriptome-wide global glycoRNAs and novel glycoRNA subtypes in both epithelial cells and B cells. Using metabolic labeling and density gradient centrifugation methods, we identified glycoRNAs predominantly below 2,000 nt in both epithelial cells and B cells. We then developed the Clier-seq (click chemistry-based enrichment of glycoRNAs sequencing) method to maximize the coverage of glycoRNAs (ranging from 50 to 2,000 nt) and utilized the HISAT-StringTie-Ballgown pipeline to predict novel glycoRNA subtypes. We also established Clier-qPCR assays (click chemistry-based enrichment of glycoRNAs RT-qPCR) to validate the specificity of the candidate glycoRNAs. We demonstrated that transfer RNAs (tRNAs), particularly tRNAs (Ser), tRNAs (Thr), tRNAs (Val), and tRNAs (Lys), are the primary targets of glycosylation. Additionally, we found that vault RNAs (vtRNAs), specifically vtRNA2-1, are glycosylated. Furthermore, we discovered several novel glycosylated long noncoding RNAs ranging from 200 to 400 nt in length. Herein, we propose a standardized bioinformatics pipeline for glycoRNA research, enabling accurate and comprehensive identification of glycoRNAs throughout the transcriptome. - Source: PubMed
Publication date: 2025/10/29
Zhu NannanYang Yan-LinLiu Yuan-TaoLu Zheng-ZhouWang YanLuo Yi-LingMeng NingYuan YanZhong QianZeng Mu-Sheng - DNA methylation may contribute to a worsening in breast cancer (BC). - Source: PubMed
Publication date: 2025/09/26
Polidoro SilviaJohansson HarrietCugliari GiovanniGuerrieri-Gonzaga AlianaAristarco ValentinaMacis DeboraCalvello MariarosariaMarabelli MonicaFeroce IreneSerrano DavideCagnacci SaraZanzottera CristinaFava FrancescaBellerba FedericaBonanni BernardoGandini Sara